Synthetic enzymes for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid and their use

ABSTRACT

The present invention relates to synthetic enzymes for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid, the use thereof for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid, DNA coding for these enzymes and microorganisms transformed with this DNA.

[0001] The present invention relates to synthetic enzymes for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid, the use thereof for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid, DNA coding for the aforementioned enzymes and microorganisms transformed therewith.

[0002] The first article relating to the degradation of eugenol was written by Tadasa in 1977 (Degradation of eugenol by a microorganism. Agric. Biol. Chem. 41, 925-929). It describes the degradation of eugenol with a soil isolate which was presumed to be Corynebacterium sp. In this process ferulic acid and vanillin were identified as intermediate degradation products and the subsequent degradation was assumed to proceed via vanillic acid and protocatechuic acid.

[0003] In 1983 another article by Tadasa and Kyahara appeared (Initial Steps of Eugenol Degradation Pathway of a Microorganism. Agric. Biol. Chem. 47, 2639-2640) on the initial steps of eugenol degradation, this time with a soil isolate which was identified to be Pseudomonas sp. In this article eugenol oxide, coniferyl alcohol and coniferylaldehyde were described as intermediates for the formation of ferulic acid.

[0004] Also in 1983 a report by Sutherland et al. appeared (Metabolism of cinnamic, p-coumaric, and ferulic acids by Streptomyces setonii. Can. J. Microbiol. 29, 1253-1257) on the metabolism of cinnamic, p-coumaric and ferulic acids by Streptomyces setonii. In this process ferulic acid was degraded via vanillin, vanillic acid and protocatechuic acid, the ring-cleaving enzymes catechol 1,2-dioxygenase and protocatechuate 3,4-dioxygenase having been indirectly identified in the cell-free extract.

[0005] In 1985 Ötük (Degradation of Ferulic Acid by Escherichia coli. J. Ferment. Technol. 63, 501-506) reported on the degradation of ferulic acid by a strain of Escherichia coli isolated from decaying bark. Here as well vanillin, vanillic acid and protocatechuic acid were found as degradation products.

[0006] isoeugenol) for the microbial oxidation of eugenol and isoeugenol. However, only when isoeugenol was used the process did produce high conversion rates; the results were very poor using eugenol.

[0007] In 1991 EP-A 453 368 appeared [“Production de vanilline par bioconversion de précurseurs benzeniques” (Production of vanillin by the bioconversion of benzene precursors)], in which the reaction to form vanillin from vanillic acid and ferulic acid using a basidiomycete—Pycnoporus cinnabarinus CNCM I-937 and 1-938—was observed.

[0008] In 1992 the Takasago Perfumery Company was granted a Japanese patent (Preparation of vanillin, coniferyl-alcohol and -aldehyde, ferulic acid and vanillyl alcohol—by culturing mutant belonging to Pseudomonas genus in presence of eugenol which is oxidatively decomposed; JP 05 227 980 21.1.1992) for the preparation of vanillin, coniferyl alcohol, coniferylaldehyde, ferulic acid and vanillyl alcohol from eugenol using a Pseudomonas mutant.

[0009] Also in 1992 U.S. Pat. No. 5,128,253 by Labuda et al. (Kraft General Foods) (Bioconversion process for the production of vanillin) was granted, in which a biotransformation process for the production of vanillin was described. Here as well the starting material was ferulic acid and the organisms used were Aspergillus niger, Rhodotorula glutinis and Corynebacterium glutamicum. The crucial feature was the use of sulphydryl components (e.g. dithiothreitol) in the medium. In 1993 the subject matter of the patent also appeared in the form of a publication (Microbial bioconversion process for the production of vanillin; Prog. Flavour Precursor Stud. Proc. Int. Conf. 1992, 477-482).

[0010] EP-A 542 348 (Process for the preparation of phenylaldehydes) describes a process for the preparation of phenylaldehydes in the presence of the enzyme lipoxygenase. Eugenol and isoeugenol are for example used as substrates. We have attempted to rework the process using eugenol, but have not succeeded in confirming the results of the reactions.

[0011] DE-A 4 227 076 [Verfahren zur Herstellung substitutierter Methoxyphenole und dafür geeigneter Mikroorganismus (Process for the production of substituted methoxyphenols and a microorganism suitable for said process)] describes the production of substituted methoxyphenols with a new Pseudomonas sp. The starting material used is eugenol and the products are ferulic acid, vanillic acid, coniferyl alcohol and coniferylaldehyde.

[0012] Also in 1995 a comprehensive review by Rosazza et al. (Biocatalytic transformation of ferulic acid: an abundant aromatic natural product; J. Ind. Microbiol. 15, 457-471) appeared on possible methods of biotransformation using ferulic acid.

[0013] The present invention relates to synthetic enzymes for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid from eugenol.

[0014] Synthetic enzymes according to the invention are for example:

[0015] a) eugenol hydroxylase,

[0016] b) coniferyl alcohol dehydrogenase,

[0017] c) coniferylaldehyde dehydrogenase,

[0018] d) ferulic acid deacylase and

[0019] e) vanillin dehydrogenase.

[0020] The invention also relates to DNA coding for the abovementioned enzymes and cosmid clones containing this DNA as well as vectors containing this DNA and microorganisms transformed with the DNA or the vectors. It also relates to the use of the DNA for the transformation of microorganisms for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid. The invention also relates to partial sequences of the DNA and functional equivalents. Functional equivalents are understood to be those derivatives in which individual nucleobases have been substituted (wobble substitutions) without resulting in any functional changes. In relation to proteins, amino acids can also be substituted without resulting in any functional changes.

[0021] The invention also relates to the individual steps for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid from eugenol, i.e. in concrete terms:

[0022] a) the process for the production of coniferyl alcohol from eugenol carried out in the presence of eugenol hydroxylase;

[0023] b) the process for the production of coniferylaldehyde from coniferyl alcohol carried out in the presence of coniferyl alcohol dehydrogenase;

[0024] c) the process for the production of ferulic acid from coniferylaldehyde carried out in the presence of coniferylaldehyde dehydrogenase;

[0025] d) the process for the production of vanillin from ferulic acid carried out in the presence of ferulic acid deacylase;

[0026] e) the process for the production of vanillic acid from vanillin carried out in the presence of vanillin dehydrogenase.

[0027] After NMG mutagenesis mutants with defects in individual stages of the catabolism of eugenol were obtained from the eugenol-utilising Pseudomonas sp. strain HR 199 (DSM 7063). Using total DNA of wild-type Pseudomonas sp. HR 199 partially digested with EcoRI a gene library was constructed in the pVK100 cosmid, which has a broad host spectrum and can also be replicated in stable form in pseudomonads. After packaging in 1-phage particles the hybrid cosmids were transduced to E. coli S17-1. The gene library comprised 1330 recombinant E. coli S17-1 clones. The hybrid cosmid of each clone was transferred by conjugation into two eugenol-negative mutants (mutants 6164 and 6165) of the Pseudomonas sp. HR 199 strain and tested for a possible capacity for complementation. In this test two hybrid cosmids (pE207 and pE115) were identified, the obtainment of which restored mutant 6165's capacity to utilise eugenol. One hybrid cosmid (pE5-1) resulted in the complementation of mutant 6164.

[0028] The complementing capacity of plasmids pE207 and pE115 was attributed to a 23 kbp EcoRI fragment (E230). A physical map of this fragment was prepared and the fragment completely sequenced. The genes vanA and vanB which code for vanillate demethylase were localised in a 11.2 kbp HindIII subfragment (H110). Another open reading frame (ORF) was found to be homologous to g-glutamyl cysteine synthetase produced by Escherichia coli. An additional ORF, which was homologous to formaldehyde dehydrogenases, was identified between the aforementioned ORF and the vanB gene. Two additional ORF's were found to be homologous to the cytochrome C subunit or the flavoprotein subunit of p-cresol methylhydroxylase, respectively produced by Pseudomonas putida. In the Pseudomonas sp. HR 199 strain, these ORF's code for a new not previously described eugenol hydroxylase which converts eugenol into coniferyl alcohol via a quinone methide derivative by a process analogous to the reaction mechanism of p-cresol methyl hydroxylase. Another ORF of an unknown function was identified between the genes of the two subunits of eugenol hydroxylase. An ORF which was homologous to lignostilbene-a,b-dioxygenase was identified in a 5.0 kbp HindIII subfragment (H50). In addition one ORF was identified which was homologous to alcohol dehydrogenases. The structural gene vdh of vanillin dehydrogenase was identified in a 3.8 kbp HindIII/EcoRI subfragment. Upstream of this gene an ORF was localised which was homologous to enoyl-CoA hydratases produced by various organisms.

[0029] The complementing capacity of plasmid pE5-1 was attributed to the joint obtainment of the 1.2 and 1.8 kbp EcoRI fragments (E12 and E18). Fragment E 12 was completely, and fragment E 18 partially, sequenced. The structural gene cadh of coniferyl alcohol dehydrogenase, which contained an EcoRI cleavage site, was localised in these fragments. Using chromatographic methods the enzyme was isolated from the soluble fraction of the crude extract of cells of Pseudomonas sp. HR 199 grown on eugenol. An oligonucleotide sequence was deduced from the specific N-terminal amino acid sequence. A corresponding DNA probe hybridised with fragment E12, in which the region of the cadh gene encoding the N-terminus was localised.

[0030] A eugenol- and ferulic acid-negative mutant (mutant 6167) was complemented by obtaining a 9.4 kbp EcoRI fragment (E 94) of the hybrid cosmid pE5-1. A physical map of this fragment was prepared. The complementing property was localised in a 1.9 kbp EcoRI/HindIII subfragment. This fragment had incomplete ORF's (they extended beyond the EcoRI and HindIII cleavage sites) which were homologous to acetyl-CoA acetyl transferases of various organisms and to the “medium-chain acyl-CoA synthetase” produced by Pseudomonas oleovorans. Fragment E 94 was completely sequenced. Downstream of the aforementioned ORF's an ORF was located which was homologous to β-ketothiolases. The structural gene of coniferylaldehyde dehydrogenase (caldh) was localised in a central position of fragment E 94. Using chromatographic methods the enzyme was isolated from the soluble fraction of the crude extract of cells of Pseudomonas sp. HR 199 grown on eugenol.

[0031] The conjugative transfer of hybrid cosmid pE207 into a large number of Pseudomonas strains resulted in the heterologous expression of the van A, van B and vdh genes and the eugenol-hydroxylase genes in the transconjugants obtained. The obtainment of the plasmid of one strain allowed it to grow using eugenol as its carbon and energy source.

[0032] Materials and Methods

[0033] Growth conditions of the bacteria. Strains of Escherichia coli were grown at 37° C. in a Luria-Bertani (LB) or M9 mineral medium (Sambrook, J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.). Strains of Pseudomonas sp. and Alcaligenes eutrophus were grown at 30° C. in a nutrient broth (NB, 0.8% by weight) or in a mineral medium NM) (Schlegel, H. G. et al. 1961. Arch. Mikrobiol. 38: 209-222). Ferulic acid, vanillin, vanillic acid and protocatechuic acid were dissolved in dimethyl sulphoxide and added to the respective medium in a final concentration of 0.1% by weight. Eugenol was added to the medium directly in a final concentration of 0.1 vol.-%, or applied on filter paper (circular filters 595, Schleicher & Schuell, Dassel, Germany) to the lids of MM agar plates. For the growth of transconjugants of Pseudomonas sp., tetracyline and kanamycin were used in final concentrations of 25 μg/ml and 300 μg/ml, respectively.

[0034] Nitrosoguanidine mutagenesis. The nitrosoguanidine mutagenesis of Pseudomonas sp. HR 199 was carried out using a modified method according to Miller (Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.). Instead of the citrate buffer, a potassium phosphate (PP) buffer (100 mM, pH 7.0) was used. The final concentration of N-methyl-N′-nitro-N-nitrosoguanidine was 200 μg/ml. The mutants obtained were screened with regard to the loss of their capacity to utilise eugenol, ferulic acid, vanillin and vanillic acid as growth substrates.

[0035] Qualitative and quantitative detection of metabolic intermediates in culture supernatants. Culture supernatants were analysed by high-pressure liquid chromatography (Knauer HPLC) either directly or after dilution with twice-distilled water. Chromatography was carried out on Nucleosil-100 C18 (7 μm, 250×4 mm). The solvent used was 0.1 vol.-% formic acid and acetonitrile.

[0036] Purification of coniferyl alcohol dehydrogenase and coniferylaldehyde dehydrogenase. The purification processes were carried out at 4° C.

[0037] Crude extract. Cells of Pseudomonas sp. HR 199 grown on eugenol were washed in a 10 mM sodium phosphate buffer with a pH of 7.5, resuspended in the same buffer and disrupted by being passed through a French press (Amicon, Silver Spring, Md., USA) twice at a pressure of 1,000 psi. The cell homogenate was subjected to ultracentrifugation (1 h, 100,000×g, 4° C.), the soluble fraction of the crude extract being obtained as the supernatant.

[0038] Anion exchange chromatography on DEAE Sephacel. The soluble fraction of the crude extract was dialysed overnight against a 10 mM sodium phosphate buffer with a pH of 7.5 containing 100 mM NaCl. The dialysate was applied to a DEAE Sephacel column (2.6 cm×35 cm, bed volumn [BV]: 186 ml) equilibrated with a 10 mM sodium phosphate buffer of a pH of 7.5 containing 100 mM NaCl at a flow rate of 0.8 ml/min. The column was washed with two bed volumes of a 10 mM sodium phosphate buffer with a pH of 7.5 containing 100 mM NaCl. The elution of coniferyl alcohol dehydrogenase (CADH) and coniferylaldehyde dehydrogenase (CALDH) was carried out with a linear salt gradient of 100 to 500 mM NaCl in a 10 mM sodium phosphate buffer with a pH of 7.5 (2×150 ml). 5 ml fractions were collected. Fractions with high CADH and CALDH activities were combined in the corresponding DEAE pools respectively.

[0039] Gel filtration chromatography on Sephadex G200. The CADH DEAE pool was concentrated in a 50 ml Amicon ultrafiltration chamber via a Diaflo ultrafiltration membrane PM 30 (both from AMICON CORP., Lexington, USA) at a pressure of 290 kPa to a volume corresponding to approx. 2% of the Sephadex G200-BV. The concentrated protein solution was applied to a Sephadex G200 column (BV: 138 ml) equilibrated with a 10 mM sodium phosphate buffer with a pH of 7.5 containing 100 mM NaCl and eluted with the same buffer at a flow rate of 0.2 ml/min. 2 ml fractions were collected. Fractions with a high CADH activity were combined in the Sephadex G200 pool.

[0040] Hydrophobic interaction chromatography on butyl Sepharose 4B. The CADH Sephadex G200 pool was adjusted to 3 M NaCl and then applied to a butyl Sepharose 4B column (BV: 48 ml) equilibrated with a 10 mM sodium phosphate buffer with a pH of 7.5 containing 3 M NaCl (flow rate: 0.5 ml/min). The column was then washed with 2 BV of a 10 mM sodium phosphate buffer with a pH of 7.5 containing 3 M NaCl (flow rate: 1.0 ml/min). CADH was eluted with a linearly decreasing NaCl gradient of 3 to 0 M NaCl in a 10 mM sodium phosphate buffer with a pH of 7.5 (2×50 ml). 4 ml fractions were collected. Fractions with a high CADH activity were combined in the HIC pool and concentrated as described above.

[0041] Chromatography on hydroxyapatite. The CALDH DEAE pool was concentrated to 10 ml in a 50 ml Amicon ultrafiltration chamber via a Diaflo ultrafiltration membrane PM 30 (both from AMICON CORP., Lexington, USA) at a pressure of 290 kPa. The concentrated protein solution was applied to a hydroxyapatite column (BV: 80 ml) equilibrated with a buffer (10 mM NaCL in a 10 mM sodium phosphate buffer with a pH of 7.0) (flow rate: 2 ml/min). The column was then washed with 2.5 bed volumes of a buffer (flow rate: 2 ml/min). CALDH was eluted with a linearly increasing sodium phosphate gradient of 10 to 400 mM NaP (in each case containing 10 mM NaCL) (2×100 ml). 10 ml fractions were collected. Fractions with high CALDH activity were combined in the CALDH HA pool.

[0042] Gel filtration chromatography on Superdex HR 200 10/30. The CALDH HA pool was concentrated to 200 μl (Amicon ultrafiltration chamber, ultrafiltration membrane PM 30) and applied to a Superdex HR 200 10/30 column (13V: 23.6 ml) equilibrated with a 10 mM sodium phosphate buffer with a pH of 7.0. CALDH was eluted with the same buffer at a flow rate of 0.5 ml/min. 250 μl fractions were collected. Fractions with high CALDH activity were combined in the CALDH Superdex pool.

[0043] Determination of coniferyl alcohol dehydrogenase activity. The CADH activity was determined at 30° C. by means of an optical enzymatic test according to Jaeger et al. (Jaeger, E., L. Eggeling and H. Sahm. 1982. Current Microbiology. 6: 333-336) with the aid of a ZEISS PM 4 spectrophotometer fitted with a TE converter (both from ZEISS, Oberkochen, Germany) and a recorder. The reaction mixture with a volume of 1 ml contained 0.2 mmol of Tris/HCl (pH 9.0), 0.4 μmol of coniferyl alcohol, 2 μmol of NAD, 0.1 mmol of semicarbazide and a solution of the enzyme (“Tris”=tris(hydroxymethyl)-aminomethane). The reduction of NAD was monitored at 1=340 nm (e=6,3 cm²/μmol). The enzyme activity was recorded in units (U), 1 U corresponding to that quantity of enzyme which metabolises 1 μmol of substrate per minute. The protein concentrations in the samples were determined according to the method described -by Lowry et al. (Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. J. Biol. Chem. 193: 265-275).

[0044] Determination of the coniferylaldehyde dehydrogenase activity. The CALDH activity was determined at 30° C. by an optical enzymatic test with the aid of a ZEISS PM 4 spectrophotometer fitted with a TE converter (both from ZEISS, Oberkochen, Germany) and a recorder. The reaction mixture of a volume of 1 ml contained a 10 mM Tris/HCl buffer (pH 8.8), 5.6 mM coniferylaldehyde, 3 mM NAD and a solution of the enzyme. The oxidation of coniferylaldehyde to form ferulic acid was monitored at 1=400 nm (e=34 cm²/μmol). The enzyme activity was recorded in units (U), 1 U corresponding to that quantity of enzyme which metabolises 1 μmol of substrate per minute. The protein concentration in the samples was determined according to the method described by Lowry et al. (Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. J. Biol. Chem. 193: 265-275).

[0045] Electrophoretic methods. The separation of protein-containing extracts was carried out in 7.4% by weight polyacrylamide gels under native conditions according to the method described by Stegemann et al. (Stegemann et al. 1973. Z. Naturforsch. 28c: 722-732) and under denaturing conditions in 11.5% by weight polyacrylamide gels according to the method described by Laemmli (Laemmli, U.K. 1970. Nature (London) 227: 680-685). Serva Blue R was used for non-specific protein staining. For specifically staining coniferyl alcohol, coniferylaldehyde and vanillin dehydrogenase the gels were placed for 20 mins in a new 100 mM PP buffer (pH 7.0) and then incubated at 30° C. in the same buffer, to which 0.08% by weight of NAD, 0.04% by weight of p-nitroblue-tetrazolium chloride, 0.003% by weight of phenazine methosulphate and 1 mM of the respective substrate had been added, until the corresponding coloured bands appeared.

[0046] The transfer of proteins from polyacrylamide gels to PVDF membranes. Proteins were transferred from SDS polyacrylamide gels to PVDF membranes (Waters-Milipore, Bedford, Mass., USA) with the aid of a semidry fast blot device (B32/33 from Biometra, Göttingen, Germany) according to the manufacturer's instructions.

[0047] Determination of N-terminal amino acid sequences. The determination of N-terminal amino acid sequences was carried out with the aid of a protein peptide sequencer (of type 477 A, Applied Biosystems, Foster City, USA) and a PTH analyser, according to the manufacturer's instructions.

[0048] Isolation and manipulation of DNA. The isolation of genomic DNA was carried out by the method described by Marmur (Marmur, J. 1961. Mol. Biol. 3: 208-218). Megaplasmid DNA was isolated according to the method described by Nies et al. (Nies, D., et al. 1987. J. Bacteriol. 169: 4865-4848). The isolation and analysis of other plasmid DNA or DNA restriction fragments, the packaging of hybrid cosmids in 1-phage particles and the transduction of E. coli. was carried out by standard methods (Sambrook, J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.).

[0049] Transfer of DNA. The preparation and transformation of competent Escherichia coli cells was carried out by the method described by Hanahan (Hanahan, D. 1983. J. Mol. Biol. 166: 557-580). Conjugative plasmid transfer between plasmid-containing Escherichia coli S17-1 strains (donor) and Pseudomonas sp. strains (recipient) and Alcaligenes eutrophus (recipient) was carried out on NB agar plates according to the method described by Friedrich et al. (Friedrich, B. et al. 1981. J. Bacteriol. 147: 198-205) or by a “minicomplementation method” on MM agar plates using 0.5% by weight of gluconate as the carbon source and 25 μg/ml of tetracylin or 300 μg/ml of kanamycin. In this process cells of the recipient were applied in one direction in the form of an inoculation line. After 5 minutes cells of the donor strains were then applied in the form of inoculation lines crossing the recipient inoculation line. After incubation for 48 h at 30° C. the transconjugants grew directly downstream of the crossing point, whereas neither the donor nor the recipient strain was capable of growth.

[0050] Hybridisation experiments. DNA restriction fragments were electrophoretically separated in an 0.8% by weight agarose gel in a 50 mM Tris, 50 mM boric acid and 1.25 mM EDTA buffer (pH 8.5) (Sambrook, J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.). The transfer of the denatured DNA from the gel to a positively charged nylon membrane (pore size: 0.45 μm, Pall Filtrationstechnik, Dreieich, Germany), the subsequent hybridisation with biotinylated or ³²P-labelled DNA probes and the production of these DNA probes was carried out according to standard methods (Sambrook, J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.).

[0051] The synthesis of oligonucleotides. Using desoxynucleoside phosphoramidites as the starting material, oligonucleotides were synthesised on a 0.2 μmol scale (Beaucage, S. L., and M. H. Caruthers. 1981. Tetrahedron Lett. 22: 1859-1862). The synthesis was carried out in a Gene Assembler Plus according to the manufacturer's instructions (Pharmacia-LKB, Uppsala, Sweden). The elimination of the protecting groups was carried out by incubation for 15 h at 55° C. in a 25 vol.-% aqueous ammonia solution. The oligonucleotides were finally purified in an NAP-5 column (Pharmacia-LKB, Uppsala, Sweden).

[0052] DNA sequencing. The determination of nucleotide sequences was carried out by the didesoxy chain termination method described by Sanger et al. (Sanger et al. 1977. Proc. Natl. Acad. Sci. USA 74: 5463-5467) using [α-³⁵S]dATP and a T7 polymerase sequencing kit (Pharmacia-LKB). 7-Deazaguanosine-5′-triphosphate was used instead of dGTP (Mizusawa, S. et al. 1986. Nucleic Acids Res.14: 1319-1324). The products of the sequencing reactions were separated in a 6% by weight polyacrylamide gel in a 100 mM Tris/HCl, 83 mM boric acid and 1 mM EDTA buffer (pH 8.3) containing 42% by weight urea, an S2 sequencing apparatus (GIBCO/BRL, Bethesda Research Laboratories GmbH, Eggenstein, Germany) being used according to the manufacturer's instructions. After electrophoresis the gels were incubated for 30 mins in 10 vol.-% acetic acid and, after washing briefly in water, dried for 2 hours at 80° C. Kodak X-OMAT AR X-ray films (Eastman Kodak Company, Rochester, N.Y., USA) were used for the autoradiography of the dried gels. In addition DNA sequences were also determined “non-radioactively” with the aid of an “LI-COR DNA Sequencer Model 4000L” (LI-COR Inc., Biotechnology Division, Lincoln, Nebr., USA) using a “Thermo Sequenase fluorescent labelled primer cycle sequencing kit with 7-deaza-dGTP” (Amersham Life Science, Amersham International plc, Little Chalfont, Buckinghamshire, England), in each case according to the manufacturer's instructions.

[0053] Various sequencing strategies were used: With the aid of synthetic oligonucleotides sequencing was carried out by the “Primer-hopping Strategy” described by Strauss et al. (Strauss, E. C. et al. 1986. Anal. Biochem. 154: 353-360). If only “universal” and “reverse primers” were used hybrid plasmids were used as “template DNA”, the inserted DNA fragments of which had been unidirectionally shortened with the aid of an “Exo III/Mung Bean Nuclease Deletion” kit (Stratagene Cloning Systems, La Jolla, Calif., USA) according to the manufacturer's instructions.

[0054] Chemicals, biochemicals and enzymes: Restriction enzymes, T4 DNA ligase, lambda DNA and enzymes and substrates for the optical enzymatic tests were obtained from C. F. Boehringer & Söhne (Mannheim, Germany) or from GIBCO/BRL (Eggenstein, Germany). [a-³⁵S]dATP and [g-³²P]ATP were obtained from Amersham/Buchler (Braunschweig, Germany). NA-type agarose was obtained from Pharmacia-LKB (Uppsala, Sweden). All the other chemicals were from Haarmann & Reimer (Holzminden, Germany), E. Merck AG (Darmstadt, Germany), Fluka Chemic (Buchs, Switzerland), Serva Feinbiochemica (Heidelberg, Germany) or Sigma Chemie (Deisenhofen, Germany).

EXAMPLES Example 1

[0055] The Isolation of Mutants of the Pseudomonas sp. HR 199 Strain with Defects in the Catabolism of Eugenol

[0056] The Pseudomonas sp. HR 199 strain was subjected to nitrosoguanidine mutagenesis in order to isolate mutants with defects in the catabolism of eugenol. The mutants obtained were classified according to their capacity to utilise eugenol, ferulic acid and vanillin as their carbon and energy source. Mutants 6164 and 6165 were no longer capable of utilising eugenol as a carbon and energy source, although, as in the case of the wild type, they were capable of utilising ferulic acid and vanillin. Mutants 6167 and 6202 were no longer capable of utilising eugenol and ferulic acid as their carbon and energy source, although, as in the case of the wild type, they were capable of utilising vanillin. The abovementioned mutants were used in the subsequent molecular-biological analyses.

Example 2

[0057] Construction of a Pseudomonas sp. HR 199 Gene Library in the Cosmid Vector

[0058] The genomic DNA of the Pseudomonas sp. HR 199 strain was isolated and subjected to partial restriction digestion with EcoRI. The DNA preparation thus obtained was ligated with vector pVK100 cut by EcoRI. The DNA concentrations were relatively high in order to accelerate the formation of concatemeric ligation products. The ligation materials were packaged in 1-phage particles which were subsequently used for transduction of E. coli S17-1. The selection of the transductants was carried out on tetracycline-containing LB agar plates. In this manner 1330 transductants were obtained which contained various hybrid cosmids.

Example 3

[0059] The Identification of Hybrid Cosmids Containing Essential Genes of Eugenol Catabolism

[0060] The hybrid cosmids of the 1330 transductants were transferred conjugatively to mutants 6164 and 6165 by a minicomplementation process. The resulting transconjugants were examined on MM plates containing eugenol for their capacity to grow again on eugenol (complementation of the respective mutant). Mutant 6164 was complemented by the obtainment of hybrid cosmid pE5-1, which contained a 1.2 kbp, a 1.8 kbp, a 3 kbp, a 5.8 kbp and a 9.4 kbp EcoRI fragment in cloned form. The E. coli S17-1 strain containing this hybrid cosmid was deposited at the “Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH” (DSM) under the number DSM 10440. Mutant 6165 was complemented by the obtainment of the hybrid cosmids pE207 or pE115 respectively. The complementing capacity was attributed to a 23 kbp EcoRI fragment which was contained in cloned form in the hybrid cosmid pE207 as the only EcoRI fragment, whereas hybrid cosmid pE115 additionally contained a 3 kbp and a 6 kbp EcoRI fragment. The E. coli S17-1 strain containing hybrid cosmid pE207 was deposited at the DSM under the number DSM 10439.

Example 4

[0061] The Analysis of the 23 kbp EcoRI Fragment (E230) of the Hybrid Cosmid pE207

[0062] Fragment E230 was isolated preparatively from EcoRI-digested hybrid cosmid pE207 and ligated to pBluescript SK⁻-DNA digested with EcoRI. Using the ligation material E. coli XL1-Blue was transformed. Following “blue-white” selection on LB-Tc-Amp agar plates containing X-Gal and IPTG, “white” transformants were obtained whose hybrid plasmids pSKE230 contained the fragment E230 in cloned form. With the aid of this plasmid and by using various restriction enzymes a physical map of the fragment E230 was prepared (FIG. 1).

[0063] By cloning subfragments of E230 in vectors pVK101 and pMP92, both of which have a broad host specturm and are also stable in pseudomonads, followed by conjugative transfer into mutant 6165, the region complementing mutant 6165 was localised in a 1.8 kbp KpnI fragment (K18). After cloning this fragment in pBluescript SK⁻ the nucleotide sequence was determined, the gene of the cytochrome C subunit of eugenol hydroxylase being identified. The gene product of 117 amino acids had an N-terminal leader peptide (MMNVNYKAVGAS-LLLAFISQGAWA) and 32.9% identity (via a region of 82 amino acids) with the cytochrome C subunit of p-cresol methylhydroxylase produced by Pseudomonas putida (McIntire et al. 1986. Biochemistry 25:5975-5981).

[0064] By cloning the KpnI subfragments of E230 adjacent to K18 in pBluescript SK⁻ and sequencing, additional open reading frames (ORF) were identified, one of which codes for the flavoprotein subunit of eugenol hydroxylase and was highly homologous to the flavoprotein subunit of p-cresol methylhydroxylase produced by Pseudomonas putida. An additional ORF was found to be highly homologous to g-glutamyl cysteine synthetase (the first enzyme in the biosynthesis of glutathione) produced by Escherichia coli (Watanabe et al. 1986. Nucleic Acids Res. 14: 4393-4400).

[0065] In the soluble fraction of the crude extract of E. coli (pSKE230) vanillin dehydrogenase was detected by specific activity staining in a polyacrylamide gel. By subcloning in pBluescript SK⁻ and analysis of soluble fractions of the crude extracts of the transformants obtained, the vanillin dehydrogenase gene (vdh) was localised in a 3.8 kbp HindIII/EcoRI subfragment of E230. The complete nucleotide sequence of this fragment was determined. The molecular weight of the vanillin dehydrogenase was 50,779, as confirmed by SDS polyacrylamide gel electrophoresis. The amino acid sequence was highly homologous to other aldehyde dehydrogenases of various origins.

[0066] Upstream of the vdh gene an additional ORF was identified which was homologous to enoyl-CoA hydratases. The calculated molecular weight of 27,297 was confirmed by SDS polyacrylamide gel electrophoresis.

[0067] By sequencing the 5.0 kbp HindIII subfragment of E230, which had also been cloned in pBluescript SK⁻, an ORF was identified which was highly homologous to the lignostilbene-a,b-dioxygenase produced by Pseudomonas paucimobilis. By complete sequencing of the fragment E230 two additional ORF's were identified which were homologous to formaldehyde-dehydrogenases (fdh) and alcohol dehydrogenases (adh) (cf. FIG. 1).

Example 5

[0068] The Analysis of the Region of Hybrid Cosmid pE5-1 Complementing Mutant 6164

[0069] Mutant 6164 was complemented by the obtainment of hybrid cosmid pE5-1 which contained a 1.2 kbp (E12), a 1.8 kbp (E18), a 3 kbp (E30), a 5.8 kbp (E58) and a 9.4 kbp (E94) EcoRI fragment in cloned form (FIG. 1). By digesting pE5-1 with EcoRI and subsequent religation a derivative (pE106) of this hybrid cosmid was obtained which only contained fragments E12, E18 and E30. Following conjugative transfer into mutant 6164 this plasmid was however capable of complementing the latter, as a result of which corresponding transconjugants were once again capable of growing on eugenol as a carbon and energy source.

[0070] After digesting plasmid pE106 with EcoRI, gel-electrophoretic separation of the digestion material in a 0.8% by weight agarose gel and transfer of the DNA to a nylon membrane, hybridisation was carried out with a ³²P-labelled oligonucleotide probe of the following sequence: ATG CAA CTC ACC AAC AAA AAA ATC GT-3′      G   G   C   T   G   G   T     G   G   C       G   G     G   T   G       G   G             G       G   G             T       G   G

[0071] The sequence of this gene probe had been deduced from the N-terminal amino acid sequence of coniferyl alcohol dehydrogenase (CADH) (see below) purified from Pseudomonas sp. HR 199. With the aid of this probe the region of the cadh gene encoding the N-terminus of the CADH was localised in fragment E12. This fragment and parts of the adjacent fragment E 18 were also sequenced and the complete sequence of the cadh gene thus determined. The amino acid sequence deduced from cadh was homologous to other alcohol dehydrogenases of class I, group II (according to Matthew and Fewson. 1994. Critical Rev. Microbiol. 20(1): 13-56).

Example 6

[0072] Purification and Characterisation of Coniferyl Alcohol Dehydrogenase

[0073] Pseudomonas sp. HR 199 was grown on eugenol. The cells were harvested, washed and disrupted with the aid of a French press. The soluble fraction of the crude extract obtained after ultracentrifugation had a specific activity of 0.24 U/mg of protein. By means of chromatography on DEAE Sephacel an 11.7-fold enrichment of CADH was obtained in a yield of 83.7%. By means of chromatography on Sephadex G200 a 6.8-fold enrichment of CADH was obtained in a yield of 11.2%. By means of chromatography on butyl Sepharose 4B a 70.6-fold enrichment of CADH was obtained in a yield of 7.8%.

[0074] With the aid of this method a preparation was obtained which displayed a band at 27 kDa according to SDS polyacrylamide gel electrophoresis. The purification factor was 64 and the yield 0.8%.

[0075] Optimum Temperature and Thermal Stability

[0076] The optimum temperature for the reaction catalysed with CADH was 42° C. The enzyme was however sensitive to heat. The half-lives were as follows: T_(1/2) (34° C.)=5 mins, T_(1/2) (39° C.)=1 min, T_(1/2) (42° C.)<1 min.

[0077] Optimum pH

[0078] The optimum pH for the reaction catalysed by CADH was 10.9 in a 25 mM MOPS buffer. At higher pH values a decrease in activity due to denaturation was observed.

[0079] Apparent Molecular Weight

[0080] The molecular weight of native CADH was determined with the aid of FPLC by gel filtration on Superdex 200HR 10/30 at 54.9 kDa, which suggests a a₂ subunit structure.

[0081] N-Terminal Amino Acid Sequence

[0082] The determination of the N-terminal amino acid sequence of the purified protein revealed the following result: 1       5         10            15               20 M Q L T N K K I V V V (G) V (S) ? (R) (I) ? (A) (E)                       (V)   (V)

[0083] (Sequence in the single letter code; ?: definition not possible; ( ): not certain; in the second row an amino acid is mentioned which may also apply)

Example 7

[0084] Purification and Characterisation of Coniferylaldehyde Dehydrogenase

[0085] Pseudomonas sp. HR 199 was grown on eugenol. The cells were harvested, washed and disrupted with the aid of a French press. The soluble fraction of the crude extract obtained after ultracentrifugation displayed a specific activity of 0.43 U/mg protein. By chromatography on DEAE Sephacel a 6.6-fold enrichment of CALDH was obtained in a yield of 65.3%. By chromatography on hydroxy-apatite a 63-fold enrichment of CALDH was obtained in a yield of 33%. By chromatography on Superdex HR 200 an 81-fold enrichment of CALDH was obtained in a yield of 13%. With the aid of this method a preparation was obtained which, according to SDS polyacryamide gel electrophoresis, displayed a band at approx. 49 kDa.

[0086] Optimum Temperature and Thermal Stability

[0087] The optimum temperature of the reaction catalysed by CALDH was 26° C. The enzyme was sensitive to heat. The half-lives were as follows: T_(1/2) (31° C.)=5 mins, T_(1/2) (34° C.)=2.5 mins, T_(1/2) (38° C.)=1 min.

[0088] Optimum pH

[0089] The optimum pH for the reaction catalysed by CALDH was 8.8 in a 100 mM Tris/HCl buffer. At this pH value the enzyme is however already unstable (87 % decrease in activity within 5 mins). At lower pH values the enzyme is more stable (e.g. pH 6.0: 50% decrease in activity within 4 hours).

[0090] Substrate Specificity

[0091] The enzyme not only accepts coniferylaldehyde (100%) but also trans-cinnamaldehyde (96.7%), sinapyl aldehyde (76.7%), p-anisaldehyde (23.1%), benzaldehyde (17.8%), 3,5-dimethoxy-benzaldehyde (7.6%) and 3-hydroxy-benzaldehyde (1.7%) as substrates.

[0092] The K_(M) value of CALDH for coniferylaldehyde is in the range between 0.007 and 0.012 mM at a V_(max) of approx. 9 to 15 U/ml. The K_(M) value of CALDH for NAD is 0.334 mM at a V_(max) of 14.2 U/ml. Compared with NAD, NADP is accepted at a rate of 4.3%.

[0093] N-Terminal Amino Acid Sequence

[0094] The determination of the N-terminal amino acid sequence of the purified protein revealed the following result: 1 S I L G L N G A P V G A E Q L G S A L (D) 20

[0095] (seqence in the one-letter code; ( ): not certain).

Example 8

[0096] Localisation and Sequencing of the Coniferylaldehyde Dehydrogenase Gene (caldh)

[0097] The N-terminal amino acid sequence was definitively assigned to an amino acid sequence deduced from the DNA sequence of fragment E94 of plasmid pE5-1. Thus the CALDH structural gene caldh is localised in E94. The amino acid sequence deduced from caldh was homologous to other aldehyde dehydrogenases.

Example 9

[0098] The Complementation of other Mutants Displaying Defects in the Catabolism of Eugenol using Hybrid Cosmids pE207 and pE5-1

[0099] Following NMG mutagenesis, mutants 6167 and 6202 had been obtained which were no longer capable of utilising eugenol and ferulic acid as their carbon and energy source (see above). The obtainment of plasmid pE207 meant that, after conjugative transfer, mutant 6202 was once again capable of utilising the aforementioned substrates. This mutant is complemented by the gene homologous to enoyl-CoA hydratase.

[0100] The obtainment of plasmid pE5-1 meant that, after conjugative transfer, mutant 6167 was once again capable of utilising the abovementioned substrates. By individually cloning the EcoRI fragments of pE5-1 in pHP 1014 and the conjugative transfer of these plasmids into mutant 6167 the complementing property was localised in fragment E94. A physical map of fragment E94 was prepared after cloning in pBluescript SK⁻ and digestion with various restriction enzymes. By cloning subfragments of E94 in the vectors pVK101 and pMP92, followed by conjugative transfer into mutant 6167, the region complementing mutant 6167 was localised in a 1.9 kbp EcoRI/HindIII fragment (EH19). After cloning this fragment in pBluescript SK⁻ and sequencing, 2 ORF's were identified which were homologous to acetyl-CoA acetyltransferases and to “medium-chain acyl-CoA synthetase” produced by Pseudomonas oleovorans. By completely sequencing fragment E94, additional ORF's were identified which were homo-logous to regulator proteins and a chemotaxis protein (cf. FIG. 1).

Example 10

[0101] Determination of the Chromosomal Coding of the Genes for the Catabolism of Eugenol in Pseudomonas sp. HR 199

[0102] Since Pseudomonas sp. HR 199 has a megaplasmid of a size of approx. 350 kbp, a hybridisation experiment was carried out to examine whether the genes for the catabolism of eugenol were localised in this megaplasmid or in the chromosome. For this purpose megaplasmid preparations of the wild type and of the mutants were separated in an 0.8% by weight agarose gel. The chromosomal and megaplasmid DNA was blotted onto a nylon membrane and then hybridised against a biotinylated HE38 DNA probe. A hybridisation signal was only obtained with the chromosomal DNA and not with the megaplasmid DNA. Thus the genes for the catabolism of eugenol in Pseudomonas sp. HR 199 are coded in the chromosome.

Example 11

[0103] The Heterologous Expression of Genes for the Catabolism of Eugenol from Pseudomonas sp. HR 199 in other Pseudomonas Strains and in Alcaligenes eutrophus.

[0104] The plasmid pE207 and a pVK101 hybrid plasmid containing fragment H110 (pVKH110) were conjugatively transferred to A. eutrophus and into Pseudomonas strains which were not capable of metabolising eugenol, vanillin or vanillic acid. The transconjugants obtained were not only examined for their capacity to grow on MM agar plates containing eugenol, vanillin or vanillic acid but also some transconjugants were incubated with eugenol in an MM liquid medium. By means of HPLC analysis of the culture supernatants some of the transconjugants were found to metabolise eugenol.

[0105] In this analysis the functional expression of the vdh gene in transconjugants of P. stutzeri, P. asplenii, Pseudomonas sp. DSM13, Pseudomonas sp. DSM15a and Pseudomonas sp. D1 was determined.

[0106] Transconjugants of the strain Pseudomonas sp. D1, which contained the plasmid pE207, were capable of growing using eugenol as their carbon and energy source. In corresponding transconjugants of P. testosteroni LMD3324, P. fluorescens TypeB, P. stutzeri DSM 50027, Pseudomonas sp. DSM 1455 and P. fragi DSM3456 functional expression of the eugenol hydroxylase genes was also observed which resulted in the secretion of intermediates of the catabolism of eugenol (coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin, vanillic acid) into the culture medium. Growth of these transconjugants on eugenol was however not observed.

1 45 1 32679 DNA Pseudomonas sp. CDS (3146)..(3997) gene = “ORF1” 1 gaattcatcc tcatggagca cttctacaag cagcaggcag gccaccctcc ccagaccgat 60 gacgtgcata ttatcgcgat cggcggaacg agctttaaac gctacctgga gctcggaaag 120 ctcctgaaca tcagagttgc cgcaattcga gataacgacg gtgactatca gcagaactgt 180 gtagcgaact acgaaggcta cctgtacgag tcggccaaga ttttcgccgc cccagatcct 240 gaccgaagca ccttcgaaat agggctgtac cgtgacaacc agaaagcctg tgacgatctc 300 tttgttgcgg gtcgcaaaaa actgaccgtg caagagtaca tgctcaaaaa taaagcggat 360 gccgctttcg agctgctgac caagaagtcc gctgaactga tcgccccgaa gtacatacag 420 gaagcgatcg aatggataag agcgtaattt tctccgtcgc aggatccggg aaaaccagcc 480 tgatcatcga gcgtctcagc cttgatcagc gggcattggt catcacttac acggacaaca 540 atcaccggca cctgcgcaac aggatcattc agagattcgg ggtgatccca tccaacatca 600 cgctcatgac gtacttctcg ttcctgcatg ggttctgcta tcggcccttg atgcaattgc 660 agctaggaac acgaggccta aatttcagac gtccgcccaa caggcagtac cccctgaacg 720 atctcaatcg gtatcgcgat ggaagcggca ggctctatca ctgccgcctc gcgaaactgc 780 tggacgttgc gcaggcctta ccggatgtgc gtgcccgcct ggagcgcttt tacgactgcc 840 tgtacgtcga cgaggtacag gatttcgcgg gtcacgactt caacctcctg ctggaggttt 900 cacgggcgaa gatcggcatg acgttcgtcg gtgatttcca ccagcacacc ttcgatacca 960 gccgagacgg agcggtaaac aaaacccttc acgacgatgc cgttcgctac gagaagcgct 1020 ttcgtgatgc cggcatttcg gtggacaagc aaacgttgaa ccgcagctgg cgatgcgcca 1080 aaacggtctg tgacttcatc agcgcaaagc tgaaaattgg cgatggacgc tcacgaggag 1140 cggggcagcc ggatcattag agttgatgac caagagcagg ccaacttgtt gcacgttgac 1200 ccaaccatcg tgaagctgtt tttgagcgaa cactacaagt acggctgcca ctccgaaaac 1260 tggggggcaa gcaaggcatg gatcacttta acgatgtctg cgttgtgatg ggcccgggta 1320 tctggaaaga ctatgtggct gagaggttac accaggccaa cccgcaaacc cgaaacaagc 1380 tgtacgtggc ctgcactagg gcgcggggtg atctgtattt cgtgcctgag aagctcttga 1440 gggccttcaa acagggaaat taggcgataa agctgaaaaa ggattttcaa gtaaagacca 1500 ctccttcctt actcgatgtc cgcttttggc cgatttctgc cagtcacgac cggcaaagaa 1560 cggccaaaag cggactgatg cggttactaa gcctgcctct tattgaagct tggtgggctt 1620 taagaatgtg gtgcgatcca gcctgatgat gttccgcttt atgcacgcag ccaagcctat 1680 cgaccgccgt ctgcacgttg taaccgacta cgcctgtgcc tttgccgctg gtggccatgg 1740 agcgtgcatc cggatcggtg agtgagactt gcccatccgg tgcttcacgt agctgctgct 1800 ccatctcctt gagcgcctgc atctgctggc ggagtttctc gattttatcc tggaggcggc 1860 tggctttggc ttcggcgaca tcggattgag ttctgtcggc ggtgtccatc gctgccagat 1920 agcggtcgat gattttatca atctggtcca tccgggcgcg cacccgctat gatccggagt 1980 cctccgatat cgatgaggcc tatctgggct ggaagagcgg ttcggtgttc tcagaccttg 2040 gcgagaacgc ggtcaagctc agcttcgggc gccaagcctt caagatcggc aacggcttcc 2100 tgatcggcga aggccacgtc gaccaaggta acgatgcggg ctactggctg gcccctacct 2160 aggcgttcga caacaccgtc ctagcccaac tggacaccgg caagctgcat gtcgacctgt 2220 tcgacctcca ggcgggcatg gatctggacg tcgccgacat caaggagaaa gtccgggtgc 2280 gcgggggcaa cgtcgagtgg cgcgacgaga cctacggcac ggtagggttc accggcttcc 2340 atacgctgga cgctgacaat ccgctgcgcg acggcatgaa tgtctacgac gtacgcgcat 2400 cgggcagccc gatccgagcc ctgccgcagg tggccctggc ggcggagtac gcctggcagc 2460 gcggcggcga ggcggacaag acgagtgagg cctggtacct acagggcagc tacacctttc 2520 gggatgcccc ctggacgcca gtgctgatgt accgtcacgc ggtcttctcc gacgactacg 2580 actccctgct gtacggctaa gggggcaaca acatgggctg gaaaggagca ttgcgttgaa 2640 acgatgctga agggcgtcac tcttttactg ctgtccgctc acgtcgaaac tgcatgattt 2700 cgggcagcct ttcttctatc cagtcggcca gcacctgaac atgagccgct acttcctggc 2760 caagcggcgt caggctgtac tcgacatgtg ggggaacgac cgggagcgaa tgtcgagcta 2820 tgaaaccgtc tccctccagg ccttgtaggg tctgcgcaag cattcttttc gctgacaccg 2880 ccgattcttc cgacgcaggt cgctgaatcg atggacaccg tccaccaaga tgatcagcac 2940 gagcacgccc agcggcttgt cacgtgcttg agcacgtccc gcgacggcat tcagcactca 3000 gcaattcccg cgccgtgctt gcatggagag actggtaagg gcggccagcg tgagtttcat 3060 ggcactaacc tttatgtatg tacttacttt tagttgctag tagggatatg gtgacgcctt 3120 catcctacga aacaagtgaa gactg atg atc gcc atc aca ggt gcc tcc gga 3172 Met Ile Ala Ile Thr Gly Ala Ser Gly 1 5 caa ctt ggt cgg ttg act ata gag gcg cta ctg aag cgc ctg cca gca 3220 Gln Leu Gly Arg Leu Thr Ile Glu Ala Leu Leu Lys Arg Leu Pro Ala 10 15 20 25 tcc gaa att att gcc ctc gtc cgg gat ccg aat aag gcc gga gac ctt 3268 Ser Glu Ile Ile Ala Leu Val Arg Asp Pro Asn Lys Ala Gly Asp Leu 30 35 40 acc gca cgt ggc atc gtg gtg cgc cag gcc gat tac aac cgg ccg gaa 3316 Thr Ala Arg Gly Ile Val Val Arg Gln Ala Asp Tyr Asn Arg Pro Glu 45 50 55 aca ctc cac cgg gcc ctg att ggg gtc aac cgg ttg ctg ttg att tcc 3364 Thr Leu His Arg Ala Leu Ile Gly Val Asn Arg Leu Leu Leu Ile Ser 60 65 70 tcc agt gag gtg ggt caa cga act gcg caa cac cgg gca gtg atc gac 3412 Ser Ser Glu Val Gly Gln Arg Thr Ala Gln His Arg Ala Val Ile Asp 75 80 85 gct gcg aag caa gaa ggt atc gag ttg ctg gct tat acg agt ctg ctt 3460 Ala Ala Lys Gln Glu Gly Ile Glu Leu Leu Ala Tyr Thr Ser Leu Leu 90 95 100 105 cat gcc gat aaa tcg gcg ctg ggc cta gcg act gaa cac cga gac acg 3508 His Ala Asp Lys Ser Ala Leu Gly Leu Ala Thr Glu His Arg Asp Thr 110 115 120 gaa cag gcc ctg aca gag tcc ggt att cct cat gtc ctg ttg cgc aac 3556 Glu Gln Ala Leu Thr Glu Ser Gly Ile Pro His Val Leu Leu Arg Asn 125 130 135 ggt tgg tat cac gag aac tac acg gcg ggc atc cca gtc gcg ctg gtt 3604 Gly Trp Tyr His Glu Asn Tyr Thr Ala Gly Ile Pro Val Ala Leu Val 140 145 150 cat ggc gtg ttg ctg ggc tgt gcc cag gat ggc ttg att gct tct gct 3652 His Gly Val Leu Leu Gly Cys Ala Gln Asp Gly Leu Ile Ala Ser Ala 155 160 165 gca cgt gct gac tac gcc gaa gca gcg gct gtg gtg ctc acc ggt gag 3700 Ala Arg Ala Asp Tyr Ala Glu Ala Ala Ala Val Val Leu Thr Gly Glu 170 175 180 185 aat cag gca ggt cgc gtc tac gag ctg gcc ggt gaa ccg gca tat acg 3748 Asn Gln Ala Gly Arg Val Tyr Glu Leu Ala Gly Glu Pro Ala Tyr Thr 190 195 200 ctc acc gaa ctg gca gct gag gtg gcg ccg caa gca gga aag acc gtc 3796 Leu Thr Glu Leu Ala Ala Glu Val Ala Pro Gln Ala Gly Lys Thr Val 205 210 215 gtg tat tcg aac cta tcc gag agc gat tac cga tct gcg ttg atc agt 3844 Val Tyr Ser Asn Leu Ser Glu Ser Asp Tyr Arg Ser Ala Leu Ile Ser 220 225 230 gcg ggc ctt ccc gat ggt ttt gcg gca ttg ctc gca gac tct gat gca 3892 Ala Gly Leu Pro Asp Gly Phe Ala Ala Leu Leu Ala Asp Ser Asp Ala 235 240 245 ggc gca gcc aag ggg tat ttg ttt gat tcc agt gga gac agt cgc aag 3940 Gly Ala Ala Lys Gly Tyr Leu Phe Asp Ser Ser Gly Asp Ser Arg Lys 250 255 260 265 ctg atc ggt cgc cca acc act ccg atg tcg gaa gcc atc gcg gca gca 3988 Leu Ile Gly Arg Pro Thr Thr Pro Met Ser Glu Ala Ile Ala Ala Ala 270 275 280 att ggc cgc taaaactgca ttttcgcgac ttgagtgaca cctgggttag 4037 Ile Gly Arg ataacccagg tgtctcgcac cgctttgggt tagtggtggg caatagcggt gtctggtcac 4097 cgcttgcccg gcggcgcgcc cgctattgga tgattctcaa cttcctggtg ccggcgtctt 4157 gttggggccc aaacaggcgg gcataacgca atgtggcatt tgcactgtcg cgcatgatgg 4217 cttctgctcg agcaccttgc ccgctaatca gcgcgtctac cacagcatga tgctgcatgt 4277 tggcaaaatt gaaccggcgg tactcttggg gaggttgcta ccgtcgacgg ccagtgaact 4337 gacagaggca aagggcaggt gttcattccg agccaatgct tcacctatgg cagcgttacc 4397 gctggcatcc acgatagctt gatggaagcg cttgttgatg tcgtggtatt cggcgaggtc 4457 gtcttcgctg acataacctt tctcaaatag ggcatcgccc tgggccaagc actgcaagag 4517 gatctcttgc gtttcactgg atagccctcg ctcggcagcc tgccttgcgg ccagtccttc 4577 aagtacccct cgaacctcca ccgcgcctgc caggtcattt ggggtcattt gccgcactgc 4637 atagccacgt gcgccttggc gatcagtaac ccttcctgtt ctagcgctcg gaacgcaatg 4697 cggataggtg tgcgccgaca ctcccaggcg ctcggcagtg gggatttcgg cgatgcgctc 4757 tcctgccggg agttcgccat ccacaatcat tttgcgcagt agattgagta ctcgctgccc 4817 gggcccgctc atttcagcct ccgattggat ccagtaatgg tttgagagaa ttttactcgc 4877 aagggatttc tgggcaatag ccccgctgat tgctggtttt tgtatgtggc gtgcgactat 4937 cgcacagaat tggatccacc ttggcgcaaa aaaactggag ctacctcatc ggtcgtggtt 4997 atattggatc ccataaggtc aagttcatag ctgattttgg ctttagatgt ccattgtgga 5057 tccaaaaaca agatcgccat tgaggaacgc gccatgtttc cgaaaaacgc ctggtatgtc 5117 gcttgcactc cggatgaaat cgcagataag ccgctaggcc gtcagatctg caacgaaaag 5177 attgtcttct atcgggggcc ggaaggacgt gttgccgcgg tagaggattt ctgccctcat 5237 cgcggggcac cgttgtccct gggtttcgtt cgcgacggta agctgatttg cggctaccac 5297 ggtttggaaa tgggctgcga gggcaaaacg ctcgcgatgc ccgggcagcg cgttcaaggc 5357 ttcccttgca tcaaaagcta cgcggtagaa gagcgatacg gctttatctg ggtatggcct 5417 ggtgatcgcg agctggcgga tccggcgctt attcaccacc tggagtgggc cgataatccg 5477 gagtgggcct atggtggcgg tctctaccac atcgcttgtg attaccgcct gatgatcgac 5537 aacctcatgg atctcaccca tgagacctat gtgcatgcct ccagcatcgg tcaaaaggaa 5597 attgacgagg caccggtcag tactcgtgtc gagggcgaca ccgtgattac cagccggtac 5657 atggataacg tcatggcccc tccgttctgg cgtgctgcgc ttcgtggcaa cggcttggcc 5717 gacgatgtac cggttgatcg ctggcagatc tgccgattcg ctcctccgag tcacgtactg 5777 atcgaagtag gtgtggctca tgcgggcaaa ggcggatatg acgcgccggc ggaatacaag 5837 gccggcagca tagtggtcga cttcatcacg ccggagagtg atacctcgat ttggtacttc 5897 tggggcatgg ctcgcaactt ccgtccgcag ggcacggagc tgactgaaac cattcgtgtt 5957 ggtcagggca agatttttgc cgaggacctg gacatgctgg agcagcagca gcgcaatctg 6017 ctggcctacc cggagcgcca gttgctcaag ctgaatatcg atgccggcgg ggttcagtca 6077 cggcgcgtca ttgatcggat tctcgcagct gaacaagagg ccgcagacgc agcgctgatc 6137 gcgagaagtg catcatgatt gaggtaatca tttcggcgat gcgcttggtt gctcaggaca 6197 tcattagcct tgagtttgtc cgggctgacg gtggcttgct tccgcctgtc gaggccggcg 6257 cccacgtcga tgtgcatctt cctggcggcc tgattcggca gtactcgctc tggaatcaac 6317 caggggcgca gagccattac tgcatcggtg ttctgaagga cccggcgtct cgtggtggtt 6377 cgaaggcggt gcacgagaat cttcgcgtcg ggatgcgcgt gcaaattagc gagccgagga 6437 acctattccc attggaagag ggggtggagc ggagtctgct gttcgcgggc gggattggca 6497 ttacgccgat tctgtgtatg gctcaagaat tagcagcacg cgagcaagat ttcgagttgc 6557 attattgcgc gcgttcgacc gaccgagcgg cgttcgttga atggcttaag gtttgcgact 6617 ttgctgatca cgtacgtttc cactttgaca atggcccgga tcagcaaaaa ctgaatgccg 6677 cagcgctgct agcggccgag gccgaaggta cccaccttta tgtctgtggg cccggcgggt 6737 tcatggggca tgtgcttgat accgcgaagg agcagggctg ggctgacaat cgactgcatc 6797 gagagtattt cgccgcggcg ccgaatgtga gtgctgacga tggcagtttc gaggtgcgga 6857 ttcacagcac cggacaagtg cttcaggtcc ccgcggatca aacggtctcc caggtgctcg 6917 atgcggccgg aattatcgtt cccgtttctt gtgagcaggg catctgcggt acttgcatca 6977 ctcgggtggt agacggagag cctgatcatc gtgacttctt cctcacggat gcggagaagg 7037 caaagaacga ccagttcacc ccctgttgct cgcgagccaa gagcgcctgt ttggtcttgg 7097 atctctaact catccccgtg tccggtcccc tgctttggtg cggcggactg tgcgcgggta 7157 agtaaacagg ctcaaccgtt tttagcggga taaccattct tgaggatgaa ggagggttat 7217 cccgctcttt tcatgcacca agccattcat agtcaccagc tgcttctacg tgctgctgcg 7277 ttacaagttt attcagaagg aaatcggaat gatcaaatcc cgcgccgctg tggcgttcgc 7337 acccaatcag ccattgcaga tcgtcgaagt ggacgtggct ccgcccaagg ccggtgaagt 7397 cctggtgcgg gtcgtggcca ccggcgtttg ccacaccgat gcctacaccc tgtccggcgc 7457 tgattccgag ggcgttttcc cctgcatcct tggtcacgaa ggcggcggca ttgtcgaagc 7517 ggtgggcgag ggcgtcacct cgctggcggt cggcgaccac gtgatcccgc tctacacggc 7577 cgaatgccgt gagtgcaagt tcttcaagtc cggcaagacc aacctgtgcc agaaagtgcg 7637 tgctactcag ggcaagggtc tgatgccgga cggcacctcc cgcttcagct acaacggtca 7697 gccgatctac cactacatgg gctgctcgac cttctccgag tacaccgtgc tgccggaaat 7757 ctccctggcg aagattccca agaatgcgcc gctggagaaa gtctgcctgc tgggctgcgg 7817 cgtgaccacc ggcattggcg cggtgctgaa cactgccaag gtggaggagg gtgctaccgt 7877 ggccatcttc ggcctgggcg gcatcggctt ggcggcgatc atcggcgcga agatggccaa 7937 ggcctcgcgc atcatcgcca tcgacatcaa tccgtccaag ttcgatgtgg ctcgcgagct 7997 gggcgccact gacttcgtca atccgaacga tcacgcgaag ccgatccagg atgtcatcgt 8057 cgagatgact gatggcggtg tggactacag cttcgagtgc atcggcaacg ttcgactcat 8117 gcgcgcagca ctcgagtgct gccacaaggg ctggggcgaa tccgtgatca tcggcgtggc 8177 gccggcgggg gccgaaatca acacccgtcc gttccacctg gtgaccggtc gcgtctggcg 8237 gggttcggcg ttcggtggcg taaagggccg caccgaactg ccgagctacg tggagaaggc 8297 acagcagggc gagatcccgc tggacacctt catcactcac accatgggcc tggacgacat 8357 caacacggcc ttcgacctga tggacgaagg gaagagcatc cgctctgttg ttcaattgag 8417 tcgctagtga agtggggtga ggaaattgga ttaggaggcg gatggttcct gccgcttaac 8477 caccttgtcc cagcttctgg ctgagatttc caagattcgg tgaaatttgc catgccgcaa 8537 actcttgctg gacggttgag tctgttatcc ggcaccgacg aattaaccct gcttcttcgg 8597 ggtggtcggg gcattgagcg tgaagccttg cgggtcgatg ttcaaggtga actggcgctg 8657 acgcctcacc cggcggcgct tggctctgcg ttgacccatc cgacaattac tacggattac 8717 gccgaggccc tgcttgagtt gatcactcgg ccggcaaccg attgtgcgca agccttggct 8777 gagctggagg agcttcaccg tttcgttcat tcgagacttg agggggagta tctctggaat 8837 ctgtccatgc ctggcagatt gccggttgat gagcaaatcc cgattgcttg gtatggacca 8897 tcaaatccag gcatgttgcg ccacgtttat cgccgtggcc tagctctgcg ttatggcaag 8957 cgaatgcaat gcatcgcagg gattcactac aactactcac tgccgccaga gcttttcgct 9017 gtcctgacca aggcagaggt cgggtctccc aagttactgg agcgccagtc agcagcttac 9077 atgcgccaaa ttcgcaacct tcggcaatac ggttggttgc tggcctactt gttcggcgct 9137 tcccccgcca tctgcaagag cttcttgggg ggcgagagag atgagctagc tcgcatgggg 9197 ggcgatacgc tttacatgcc ctatgcaacc agcttgcgca tgagtgacat cgggtaccgc 9257 aaccgtgcca tggatgatct atctcccagc ctgaatgatc tgggtgccta tattcgcgat 9317 atttgccgtg ctcttcacac tcccgatgcc cagtaccagg cgctgggtgt gtttgcacag 9377 ggcgagtggc ggcagttaaa cgccaatcta ttgcagttgg atagtgagta ctacgcactg 9437 gcgcgaccga agtcagcgcc cgagcggggg gagcgaaacc tggatgctct cgctaggcgt 9497 ggagtccagt atgtggagct gcgcgcactg gatctcgatc cattctcccc gttaggcatt 9557 ggcctgacct gcgccaagtt cctcgatggc tttttgcttt tctgcttgtt gtctgaggcg 9617 ccggttgatg atcgaaatgc ccagcgttca agaccgggaa aatctgagcc tggccggcaa 9677 gtacgggcgt cacctggctt aaagctgcat cggaatggtc agtccattct cctcaaggat 9737 tgggcgcagg aagtgttgac ggaggttcag gcctgtgtgg aattgctcga cagtgcaaat 9797 gggggctcat ctcacgcatt ggcttggtca gcacaggagg aaaaggtgct taatccggat 9857 tgtgcgccat cagctcaggt gctcgcagag atacacagac acggtgggag cttcacggca 9917 tttggtcgcc aattagctat cgaccatgca aaacacttca gtgcctcctc gcttgaggct 9977 ggcgtagcca aagcgcttga cctccaggcg acgtcgtctc tgcgcgagca gcatcaattg 10037 gaggccaacg accgtgcgcc attttctgac taccttcagc aattctccct ggctttcggt 10097 caatccgtcg gcgcctctcg tgcgcccaac cctaccgcgc acctcatcga tctgacccct 10157 cctgtctaag gttgtcgtgg gagcagatcc gtgggccgag cttcctccag ggcctggccg 10217 cagcgatcca gttgctaggt ccctatgctc ttgcataggg taaaaattag ttattgtgtt 10277 taacgaaacg tctggcatac tggctttagg cacgagcttc cacgccgaag ttgagagcgt 10337 catgaacgat ttttcgtgtg gagagacgat gcccgatgcg gtcgacgagg ttcaggtcct 10397 aatggcagtg ccggcggcta aacggaacgt gccgtatttt gaggcttgga gcgtggtgaa 10457 gcagcttggc tgctccctgg gcctgtcagg atcacgctgt gtcggcagtg acacttcaaa 10517 acaagaaggg cattaagatg atgaatgtta attataaggc tgtcggggcg agcctactcc 10577 tcgccttcat ctctcaggga gcttgggcag agagccccgc agcctctggc aatacccctg 10637 acatttatcg aaagacctgc acctactgcc atgagcctac tgtcaacaat ggccgggtca 10697 ttgcccgaag cctcgggccg actctgcgag ggcgccagat ccctccacag tacacggagt 10757 acatggtgcg tcatggacgc ggggcaatgc ctgcattctc tgaagcagaa gtgcctccgg 10817 cggagctgaa agttctgggc gattggattc agcaaagcag tgctcccaaa gacgctggag 10877 tcgcgccatg actacccgtc gcaactttct aataggcgcg tcgcaggtgg gggcattggt 10937 gatgatgtcg ccgaaattgg tcttccgtac gccgctcaag cagaagcccg tgcgcatcct 10997 gtcgaccggg ctggccggtg agcaagagtt tcactcgatg cttcgcgcgc gattgaccca 11057 tacgggtcag gtcgacatcg cgtcggtacc gctggacgca gctatttggg cttctcccgc 11117 tcgacttgcc caggcaatgg atgcgttgaa tggtacgcgt ctgatcgctt ttgttgagcc 11177 caggaacgaa ttgatactga tgcaattctt gatggatcgc ggggctgcgg tgcttattca 11237 aggtgagcat gcggtggaca gcaagggggt ctctcggcac gactttctga gtaccccatc 11297 cagtgcggga attggagggg cgctagccga cagcctggca aaagggggct cgccgttctc 11357 tatttccgtc cgagcgcttg gctcggtaac tgctcagcca agaagtaatc agagtgaggt 11417 ggccacccac tggacgaccg ctctggggac ctattatgcc gatatcgcag tggggcgctg 11477 ggagccgcag cgcgaagtgg ccagctatgg aagtggacta atcatggcgg aacggcttga 11537 tcgtgttgcc tcaaccttca ttgcagatct ctgagtcagg gtattgatat ggaaagcacc 11597 gtagttcttc ccgagggtgt caccccggag cagttcacca aagccatcag cgagttccgt 11657 caggtattgg gtgaggacag tgttcttgtc actgctgaac gagttgttcc ctatacgaaa 11717 ctcctcattc ctacacagga tgatgcccag tacaccccgg ccggtgcctt gactccttct 11777 tcggtggagc aggtccagaa agtcatgggg atctgcaata agtacaagat cccggtatgg 11837 ccaatctcta ccggtcggaa ctgggggtat gggtccgctt cgcctgcaac tcctgggcag 11897 atgattcttg accttcgcaa gatgaacaag atcattgaga tcgatgttga ggggtgtact 11957 gccctgctcg agccgggcgt tacctaccag cagcttcacg attacatcaa ggagcacaat 12017 ctgcccttga tgctggatgt gccgactatt gggcctatgg ttggcccggt gggtaacacg 12077 ctggatcgag gcgttggtta tacgccgtac ggcgagcact tcatgatgca gtgtggtatg 12137 gaagtcgtca tggccgatgg cgaaatcctc cgtactggta tgggctcggt gcccaaagcc 12197 aagacttggc aggcattcaa atggggctat ggtccatatc tggacggtat ctttacccag 12257 tccaactttg gtgttgtgac aaagctcggg atttggttga tgcccaagcc gccagtgatc 12317 aagtcgttta tgatccgtta tcccaatgaa gctgatgtgg ttaaggcaat tgatgctttt 12377 cgcccgctgc gtattactca gctgattcct aacgtcgttt tgttcatgca cggcatgtac 12437 gaaacggcaa tctgccggac gcgtgctgag gttacttcgg acccaggtcc tatttctgaa 12497 gcggacgccc gcaaagcatt caaagagcta ggcgttggct actggaacgt ttacttcgcg 12557 ctttacggca cagaagagca gatagccgtc aatgaaaaga tcgtccgcgg catcctcgaa 12617 ccgacggggg gtgagatcct caccgaagag gaggctggag ataacattct tttccatcac 12677 cataagcagc tcatgaacgg cgagatgaca ttggaggaaa tgaatatcta ccagtggcgc 12737 ggagcaggtg gcggtgcttg ctggtttgca ccggttgctc aggtcaaggg gcatgaggca 12797 gagcagcagg tcaagcttgc tcagaaggtg cttgcaaagc atgggttcga ttacacggcg 12857 ggctttgcga ttggttggcg cgatcttcac catgtgatcg atgtgctgta cgaccgtagc 12917 aatgccgacg agaaaaagcg cgcttacgct tgctttgatg aattgatcga cgtctttgcg 12977 gccgaaggct ttgcaagtta caggaccaat attgccttta tggacaaagt cgcctctaag 13037 ttcggcgctg agaataagag ggtcaatcag aagatcaagg ctgcccttga tccaaacggc 13097 atcatcgctc ccggcaagtc gggcattcat cttcccaaat aatgcgtgtt cgtgaggcgg 13157 ctgctagccg cctcatttga agaaagagtc gtatcggcga tgcatgatgc gtcgttcgct 13217 ctcggctgtt gattcttcga aagaagcgta tgggggggga atgattgcaa tcactgcggg 13277 caccggaagt cttggtcggg ctatcgttga gcgactaggg gactgcggtc ttatcggtca 13337 agttcgattg acggctcgcg atcctaaaag gcttcgtgcc gctgccgagg aagggtttca 13397 ggtcgctaag gcggattacg ccgatattgg gagtcttgac caggcattac agggggtaga 13457 cgtattactc ctgatttctg gtactgcacc caatgaaata aggatccaac agcataagtc 13517 ggtcatcgac gcggcaaaac gaaacggcgt gtcgcgtatt gtgtatacca gcttcataaa 13577 tccaagtact cgcagcaggt ctatttgggc ctccattcat cgtgaaactg agacttacct 13637 caggcagtct ggggtgaagt ttacgattgt ccgaaataat cagtatgcgt ctaacctgga 13697 tctgttgctg ctgagggctc aagacagcgg aatatttgcc attcccgggg cgaaggggcg 13757 ggtggcgtac gtctctcatc gcgacgttgc cgctgccatc tgtagtgtcc tgacgaccgc 13817 cggacacgat aacaggatct accagctcac aggctctgag gctctcaatg ggctcgagat 13877 cgcggagatt cttggtgggg tgctcgggcg tccagtgcgc gcgatggatg cctcgcctga 13937 cgagtttgct gccagctttc gcgaggctgg attccctgag tttatggttg aaggcctact 13997 aagcatttat gccgcttcag gtgctgggga gtaccaatcc gtcagtcctg atgttgggtt 14057 gttgacggga cgacgtgccg aatcgatgcg aacttacata cagcgtctag tttggccttg 14117 agggaggtga ccgacgtatg aaggcttatg agcttcacaa gatttcggaa caggtagagg 14177 tcaggctcca gccaactcgg ccccgcccgc agttgaatca tggcgaggtc ctcatcaggg 14237 tccatgcagc ctcgctcaac tttcgcgatt tgatgatctt ggccggtcgc tatccgggtc 14297 aaatgaaacc cgatgtgatc ccgctgtccg atggtgctgg cgagattgtg gaggtcgggc 14357 ctggcgtatc ttcggaggtg cagggtcagc gcgtagccag cacctttttc cctaactggc 14417 gggccggaaa gattaccgag ccggctattg aggtgtcgtt gggcttcggt atggacggga 14477 tgctcgcgga atacgttgct ctgccctatg aggcaacgat accgataccg gagcacctgt 14537 cgtacgagga ggctgcaaca ttgccttgcg cggcgctaac cgcttggaat gcgttgaccg 14597 aagtggggcg tgtcaaggcc ggtgatacgg tcttgttgct tggcactggc ggtgtctcga 14657 tgttcgcgtt gcagttcgcc aagctcttgg gggcgacggt cattcacacc tcgagcagtg 14717 aacaaaagct ggagagggtg aaagcgatgg gggctgatca tctgatcaac taccgcaatt 14777 cgccagggtg ggaccgtact gtcctggatc tcaccgcggg gcgaggggtt gacctggtag 14837 tcgaggtagg gggggcgggg accttggagc gctcacttcg tgcggtcaag gtaggcggta 14897 ttgtcgccac gattgggcta gtggctggcg ttggcccgat tgacccattg ccgcttatct 14957 ccagggctat tcagctctcg ggcgtctatg tcggttcccg ggaaatgttt ctctcaatga 15017 acaaagccat tgcatcagcc gaaatcaagc cagtgatcga ttgctgcttc cccatcgacg 15077 aggttggaga tgcttatgag tacatgcgta gcggcaatca ccttggcaaa gtagttatca 15137 cgatctaact gccgctaaac ccgttgtgcg gcaatttgcg ggagctagta ccgggctttc 15197 ggtttggctc ttggatggtc ttcgcatgca cgctttacga agggggccag ggacagacgc 15257 cccggggcgt aatcaatggc cttgcgtgca ggctctcacc gtcgtgatcg ggattggaaa 15317 ttcgtgcgag gacagcggcc acgtaccggc gccctgaagg gctggaaggt tggagtttcg 15377 ttaaggtctg gtacccagca gccatggaga gcggccctta gccggaatgg cagcttgatg 15437 gttgccacgg gaccagactg gatgtcttga gtgtcgagaa ttaccagatc gctgcgattt 15497 tcatcgaggc gaccaaccac ggtcagcaag tacccgtcac cttcggcggc ggtcggactt 15557 ctagggacga aggccggctc ctgggccgcc gaggcttcgc cggagtacca gaggtcgtag 15617 tcacctcggt ggttgtccca gatgccgagt gagttgtacg cgaatatctt ctcggcctgc 15677 tgatgcgcaa gtggtttgcg tggatcgtcc acccccataa agccatagcg gttgcattgc 15737 agggcgaacg aagaatccat gattggcatt tccgcaaaga aatcgtgtag ccgggttcgc 15797 ttgatctcgt cgctgctgct atcgaggtca atttcccaac gagtcaggcg tggtacggct 15857 ttctcagggg cgaagggttg gttttgtgag ttggggaagg ggaacggcag gatttcactt 15917 tccataaggt cgatataaat cttggttccg acttcccaag cattcacaac atgaaatacc 15977 cagagcgccg gtgccttgag ccagcgaatc agactgccct ggcgcggcgc gagtacgcca 16037 atgtagctgc ccagttccgg ctcccacata taaattggct gtttcgcctt gaggcgggac 16097 aggctgttgg tggccggcat aattgggaaa atggaccaat ttcgggtaat ggcaaagtcg 16157 tgcatgaatg cgccataggg ctgctcaaac caagtttcat gtgtcacctt gccgtgcttg 16217 tcgacaatgt aataggccat gtctggagtt gcttcgccct tagctgccga accgaagaac 16277 aacaagtcac ccgtttccgg gtcatatttt ggatgggcgg tgtgggtttg gctggtaact 16337 tggccgtcgt agtcgaagtg tccgcgagtt tcaagtgtac gaggatccag ttcgtacggt 16397 aggccgtctt ccttcaccgc cagcaccttg ccgtgatggc taatgatgct tgtattggca 16457 acggtgcggt ctagtccttt tacactggtg tcgtcggtat aggggtttct gtacatgcca 16517 aatagcgatt ttcgcgctag tcgttcggcc gtgaatcgag cggttttaac ccagcgactg 16577 atgaagtcga catgaccatc ttcgaagtgg aaggcagagg ccattccatc tccatctatg 16637 aaggtgtgga atttttgtgg ggtaacttga ggctctggcg tattacggta gaacgttcca 16697 tttattgatt ttgggatttc gccgtcaacc tctagatcga acaagtctgc ctctatacgg 16757 gtggggagaa gtgttcctac taattgcggg tcgttgcggt tgaatctcgc catggcacgg 16817 tctcctttgt tgttctgaat ggcctaaatg cgcggcttgc cgggttggag tttatgttta 16877 ggactgaccg gatttcatgt gtgccggtga agtgaagatg tctgtgagtg caatggtggt 16937 ggtattgaaa atgggccgag gctggcctat tgtttagaat ttcaagaatg acaactattc 16997 ggtggcggcg tatgtccatt cactctgagg ggatcactct cgcggattcg ccgctgcatt 17057 gggcgcatac cctgaatgga tcaatgcgta ctcatttcga agtccagcgt cttgagcggg 17117 gtagaggtgc ctcccttgcc cgatctagat ttggcgcggg tgagctgtac agtgccattg 17177 caccaagcca ggtacttcgc cacttcaacg accagcgaaa tgctgatgag gctgagcaca 17237 gctatttgat tcagatacga agtggcgctt tgggcgttgc atccggcgga agaaaggtga 17297 tcttggcaaa tggtgattgc tccatagttg atagtcgcca agacttcaca ctttcctcga 17357 actcttcgac ccaaggtgtc gtaatacgct ttccggtgag ttggctggga gcgtgggtgt 17417 ccaatccgga ggatcttatc gcccgacgag ttgatgctga ggtagggtgg ggtagggcgc 17477 taagcgcatc ggtttctaat ctagatccat tgcgcatcga cgatttaggt agcaatgtaa 17537 atggcattgc agagcatgtt gctatgttaa tttcactagc aagttctgcg gttagttctg 17597 aagatggggg tgtggctctt cggaaaatga gggaagtgaa gagagtactc gagcagagtt 17657 tcgcagacgc taatctcggg ccggaaagtg tttcaagtca attaggaatt tcgaaacgct 17717 atttgcatta tgtctttgct gcgtgcggta cgacctttgg tcgcgagctg ttggaaatac 17777 gcctgggcaa agcttatcga atgctctgtg cggcgagtga ctcgggtgct gtgctgaagg 17837 tggccatgtc ctcaggtttt tcggattcaa gccatttcag caagaaattt aaggaaagat 17897 acggtgtttc gcctgtctcc ttggtgaggc aggcttgatt tcccatagcg ttattgcggt 17957 cgtcgttgca aatgcggacc tgcgtgatca tcaaggctaa gactgccaca ttaggtgtcg 18017 actcgagcgt ccctctatcc gcctgaccgc gctccgtccc tagtacctag gaaattgagt 18077 gggcctactt gccagggcca gttggattcg gtgctggtga gcgctgcggg tgacagaatc 18137 ctgatcgtgg cgatcacgat ggcgataaag ttgcccggtg tcgtagatcg cagggtgacc 18197 aagacgggga ctcatggcgc ggatcccgcc agtgatgcct tcgcatgacg ccacctctct 18257 cctccgctca gccttcatgc ctgactaatt aagtcgtata tcaatctggc tctgtgccgc 18317 attcagttcc tccagctgca ttgtctctcg gcgggagggc attcccctgc attggccaaa 18377 tgggtcccct tgttcacgac cggacaagcg caccgtgctg cccgttcgtc gtgtgccctg 18437 tcaaaaagcc tggcgacgaa agggcggcag gccgcatggc cacggctggg cggtaactga 18497 tgcttgcgtt aatcgttaac cgtttgaaat tccttgccaa atttcggcga gagaatcatg 18557 cgggtacgcc tttccgtgcg ctttgatctg cgcttccgtg ccttgaatca gaaaaatagt 18617 taattgacag aactataggt tcgcagtagc ttttgctcac ccaccaaatc cacagcactg 18677 gggtgcacga tgaatagcta cgatggccgt tggtctaccg ttgatgtgaa ggttgaagaa 18737 ggtatcgctt gggtcacgct gaaccgcccg gagaagcgca acgcaatgag cccaactctc 18797 aatcgagaga tggtcgaggt tctggaggtg ctggagcagg acgcagatgc tcgcgtgctt 18857 gttctgactg gtgcaggcga atcctggacc gcgggcatgg acctgaagga gtatttccgc 18917 gagaccgatg ctggccccga aattctgcaa gagaagattc gtcgcgaagc gtcgacctgg 18977 cagtggaagc tcctgcggat gtacaccaag ccgaccatcg cgatggtcaa tggctggtgc 19037 ttcggcggcg gcttcagccc gctggtggcc tgtgatctgg ccatctgtgc cgacgaggcc 19097 acctttggcc tgtccgagat caactggggc atcccgccgg gcaacctggt gagtaaggct 19157 atggccgaca ccgtgggtca ccgcgagtcc ctttactaca tcatgactgg caagacattt 19217 ggcggtcagc aggccgccaa gatggggctt gtgaaccaga gtgttccgct ggccgagctg 19277 cgcagtgtca ctgtagagct ggctcagaac ctgctggaca agaaccccgt agtgctgcgt 19337 gccgccaaaa taggcttcaa gcgttgccgc gagctgactt gggagcagaa cgaggactac 19397 ctgtacgcca agctcgacca atcccgtttg ctcgatccgg aaggcggtcg cgagcagggc 19457 atgaagcagt tccttgacga gaaaagcatc aagccgggct tgcagaccta caagcgctga 19517 taaatgcgcc ggggccctcg ctgcgccccc ggccttccaa taatgacaat aatgaggagt 19577 gcccaatgtt tcacgtgccc ctgcttattg gtggtaagcc ttgttcagca tctgatgagc 19637 gcaccttcga gcgtcgtagc ccgctgaccg gagaagtggt atcgcgcgtc gctgctgcca 19697 gtttggaaga tgcggacgcc gcagtggccg ctgcacaggc tgcgtttcct gaatgggcgg 19757 cgcttgctcc gagcgaacgc cgtgcccgac tgctgcgagc ggcggatctt ctagaggacc 19817 gttcttccga gttcaccgcc gcagcgagtg aaactggcgc agcgggaaac tggtatgggt 19877 ttaacgttta cctggcggcg ggcatgttgc gggaagccgc ggccatgacc acacagattc 19937 agggcgatgt cattccgtcc aatgtgcccg gtagctttgc catggcggtt cgacagccat 19997 gtggcgtggt gctcggtatt gcgccttgga atgctccggt aatccttggc gtacgggctg 20057 ttgcgatgcc gttggcatgc ggcaataccg tggtgttgaa aagctctgag ctgagtccct 20117 ttacccatcg cctgattggt caggtgttgc atgatgctgg tctgggggat ggcgtggtga 20177 atgtcatcag caatgccccg caagacgctc ctgcggtggt ggagcgactg attgcaaatc 20237 ctgcggtacg tcgagtgaac ttcaccggtt cgacccacgt tggacggatc attggtgagc 20297 tgtctgcgcg tcatctgaag cctgctgtgc tggaattagg tggtaaggct ccgttcttgg 20357 tcttggacga tgccgacctc gatgcggcgg tcgaagcggc ggcctttggt gcctacttca 20417 atcagggtca aatctgcatg tccactgagc gtctgattgt gacagcagtc gcagacgcct 20477 ttgttgaaaa gctggcgagg aaggtcgcca cactgcgtgc tggcgatcct aatgatccgc 20537 aatcggtctt gggttcgttg attgatgcca atgcaggtca acgcatccag gttctggtcg 20597 atgatgcgct cgcaaaaggc gcgcggcagg tcgtcggtgg tggcttagat ggcagcatca 20657 tgcagccgat gctgcttgat caggtcactg aagagatgcg gctctaccgt gaggagtcct 20717 ttggccctgt tgccgttgtc ttgcgcggcg atggtgatga agaactgctg cgtcttgcca 20777 acgattcgga gtttggtctt tcggccgcca ttttcagccg tgacgtctcg cgcgcaatgg 20837 aattggccca gcgcgtcgat tcgggcattt gccatatcaa tggaccgact gtgcatgacg 20897 aggctcagat gccattcggt ggggtgaagt ccagcggcta cggcagcttc ggcagtcgag 20957 catcgattga gcactttacc cagctgcgct ggctgaccat tcagaatggc ccgcggcact 21017 atccaatcta aatcgatctt cgggcgccgc gggcatcatg cccgcggcgc tcgcctcatt 21077 tcaatctcta acttgataaa aacagagctg ttctccggtc ttggtggatc aaggccagtc 21137 gcggagagtc tcgaagagga gagtacagtg aacgccgagt ccacattgca accgcaggca 21197 tcatcatgct ctgctcagcc acgctaccgc agtgtgtcga ttggtcatcc tccggttgag 21257 gttacgcaag acgctggagg tattgtccgg atgcgttctc tcgaggcgct tcttcccttc 21317 ccgggtcgaa ttcttgagcg tctcgagcat tgggctaaga cccgtccaga acaaacctgc 21377 gttgctgcca gggcggcaaa tggggaatgg cgtcgtatca gctacgcgga aatgttccac 21437 aacgtccgcg ccatcgcaca gagcttgctt ccttacggac tatcggcaga gcgtccgctg 21497 cttatcgtct ctggaaatga cctggaacat cttcagctgg catttggggc tatgtatgcg 21557 ggcattccct attgcccggt gtctcctgct tattcactgc tgtcgcaaga tttggcgaag 21617 ctgcgtcaca tcgtaggtct tctgcaaccg ggactggtct ttgctgccga tgcagcacct 21677 ttccagcgcg caattgagac cattctgccg gacgacgtgc ccgcaatctt cactcgaggc 21737 gaattggccg ggcggcgcac ggtgagtttt gacagcctgc tggagcagcc tggtgggatt 21797 gaggcagata atgcctttgc ggcaactggc cccgatacga ttgccaagtt cttgttcact 21857 tctggctcta ccaaactgcc taaggcggtg ccgactactc agcgaatgct ctgcgccaat 21917 cagcagatgc ttctgcaaac tttcccggtt tttggtgaag agccgccggt gctggtggac 21977 tggttgccgt ggaaccacac cttcggcggc agccacaaca tcggcatcgt gttgtacaac 22037 ggcggcacgt actaccttga cgacggtaaa ccaaccgccc aagggttcgc cgagacgctt 22097 cgcaacttga gcgaaatctc tcccactgcg tacctcactg tgccgaaagg ctgggaggaa 22157 ttagtgggtg cccttgagcg agacagtacc ctgcgcgaac gcttcttcgc tcgcatgaag 22217 ctgttcttct tcgcggcggc tgggttgtcg caagggatct gggatcgttt ggaccgggtc 22277 gctgaacagc actgtggtga gcgcattcgc atgatggcgg gtctgggcat gacggagact 22337 gctccttcct gcacttttac caccggaccg ctgtcgatgg ctggttacat tgggctgcca 22397 gcgcctggct gcgaggtcaa gctcgttccg gtcgatggga aattggaagg gcgtttccat 22457 ggtccgcacg tcatgagcgg ctactggcgt gctcctgaac aaaatgccca agcgttcgac 22517 gaggaaggct attactgctc cggtgatgcc atcaaattgg cagatcctgc cgatcctcag 22577 aaaggtctga tgtttgacgg tcgaattgct gaagacttca agctgtcctc aggggtattt 22637 gtcagcgttg ggccattgcg cacgcgggcg gttctggaag gcggctctta cgtcctggac 22697 gtagtggttg ctgctcctga tcgtgaatgc cttggattgc tcgtgtttcc gcgtcttctc 22757 gactgccgtg ccttgtcggg gctaggaaaa gaggcgtcgg acgccgaggt gcttgccagt 22817 gagccggttc gggcctggtt tgctgactgg ctcaaacgac tcaatcgaga agcaactggc 22877 aatgccagtc gcatcatgtg ggtagggctc ctcgatacgc cgccgtcgat tgataagggc 22937 gaggtcactg acaagggctc gatcaaccag cgcgctgttt tgcaatggcg gtcggcgaaa 22997 gttgatgcgc tgtatcgtgg tgaagatcaa tccatgctgc gtgacgaggc cacactgtga 23057 gttggtcagg gggggcttac tcggcgtttt ccgacactgc gttggttgcg gcagtgcgca 23117 ccccctggat tgattgcggg ggtgccctgt cgctggtgtc gcctatcgac ttaggggtaa 23177 aggtcgctcg cgaagttctg atgcgtgcgt cgcttgaacc acaaatggtc gatagcgtac 23237 tcgcaggctc tatggctcaa gcaagctttg atgcttacct gctcccgcgg cacattggct 23297 tgtacagcgg tgttcccaag tcggttccgg ccttgggggt gcagcgcatt tgcggcacag 23357 gcttcgaact gcttcggcag gccggcgagc agatttccca aggcgctgat cacgtgctgt 23417 gtgtcgcggc agagtccatg tcgcgtaacc ccatcgcgtc gtatacacac cggggcgggt 23477 tccgcctcgg tgcgcccgtt gagttcaagg attttttgtg ggaggcattg tttgatcctg 23537 ctccaggact cgacatgatc gctaccgcag aaaacctggc gcgcctgtac ggaatcacca 23597 ggggagaagc taattcctac gcggtaagca gcttcgagcg cgcattgagg gcgcaagagg 23657 agaaatggat tgaccaagag atcgtggctg ttacggatga acagttcgat ttagagggct 23717 acaacagtcg agcaattgaa ctgcctcgga aggcaaaatt gttgatcgtg acagtcatcc 23777 gcggcctagc agtctttgaa gccctttccc gattgaagcc tgttcattct ggcggggtgc 23837 agactgcggg caacagctgt gccgtagtgg acggcgccgc ggcggctttg gtggctcgag 23897 agtcgtctgc gacacagccg gtcttggcta ggatactggc tacctccgta gtcgggatcg 23957 agcccgagca tatggggctc ggccctgcgc ccgcgattcg cctgctgctt gcgcgtagtg 24017 atcttagttt gagggatatc gacctctttg agataaacga ggcgcaggcc gcccaagttc 24077 tagcggtaca gcatgaattg ggtattgagc actcaaaact taatatttgg ggcggggcca 24137 ttgcacttgg acacccgctt gccgcgaccg gattgcgtct ctgcatgacc ctcgctcacc 24197 aattgcaagc taataacttt cgatatggaa ttgcctcggc atgcattggt gggggacagg 24257 ggatggcggt tcttttagag aatccccact tcggttcgtc ctctgcacga agttcgatga 24317 ttaacagagt tgaccactat ccactgagct aacgggcatc tcctttgttg ctttgaggtg 24377 gcgcacgaag gagggctcga aaatctctgc taaaaacaag aagaaggaac agggaacatg 24437 attagtttcg ctcgtatggc agaaagttta ggagtccagg ctaaacttgc ccttgccttc 24497 gcactcgtat tatgtgtcgg gctgattgtt accggcacgg gtttctacag tgtacatacc 24557 ttgtcagggt tggtggaaaa gagcgcgata gctggtgagt tgcgggcgaa aattcaggaa 24617 ctgaaggttc tggagcagcg cgccttattc atcgccgatg aagggtcgct gaagcagcgc 24677 tcgatcctcc taagtcaggt gatagctgaa gttaatgatg ctatagatat ttttgacttt 24737 cagcgcggac gatctgagtt acttaaattc gctgcttctt cgcgcgaagc aagttactcc 24797 attgaggtcg gtagtaacgc tgcggccgat aagttgcagt cgggcgaacc aagtgacgca 24857 ttgatggttg ccgataaaaa gctgaatgtt gagtatgagc aattgagttc tgctgtgaat 24917 gcactgatgg ggcatttaat tgaggatcag aatgaaaaag ttccactaat ctactatatg 24977 cttggcggcg taactttgtt tacgatgctc atgagtgctt attcggtctg gttcatttcg 25037 cgtcagttag ttccgccatt aaagtcgacg gtgcagcttg ccgagcggat tgcatcaggc 25097 gacttggctg atgtcgggga cagcaggcgc aaggatgaaa tcggtcagtt gcaaagtgca 25157 actaggcgga tggcgattgg actgcgtaat ctggtcggtg atattggtca aagtcgtgcg 25217 caactggttt catcgtccag cgacctttcg gccatctgtg ctcaggctca gattgatgtc 25277 gagtgccaga agctttcggt cgcccaggtc tctaccgccg tgaacgagtt ggttgaaacc 25337 gtccaggcaa tagcaaaaag caccgaagag gcagcaacag tcgccgtctt ggccgatgaa 25397 aaggcacgcg gtggtgaaag tgtcgttaac aaggccgttg atttcattga gcacctctcc 25457 ggagatatgg cggaactggg agacgcaatg gagcggcttc agaacgacag tgcgcagatc 25517 aataaggtag tagacgtcat taaggctgtg gcggagcaga ccaatctgct agccctgaat 25577 gcggcgatag aggcggcccg tgcaggagag cagggcaggg gctttgcggt cgtggcggat 25637 gaggttcgtg ctttggcgat gcgcacccaa caatcgacca aagaaattga gaggctagtg 25697 gtttcattgc agcagggaag tgaagctgcg ggcgagttga tgcggcgtgg caaggtccgg 25757 acgcatgacg tcgttggatt ggcccagcaa gccgcgcgcc gcgctactcg aaattaccca 25817 gctgtcgccg gcatccaagc gatgaactat cagatcgccg ctggagcaga gcagcaaggg 25877 gctgctgtgg ttcaaatcaa ccagaatatg cttgaagtgc ataagatggc tgacgagtcc 25937 gccattaaag cgggacagac catgaagtca tcgaaggagc ttgctcacct cggcagtgcg 25997 ctacaaaaat ccgttgatcg attccagctg tagcgctccg ggtggctgaa acgcgcattt 26057 tcgttaaggt cttcagcgcg gtctgctggt gcgtgggccg ctagcctaac tgttgcgctt 26117 caggctccgc atggatcttg tgcagcagca atagcaattg ttcacgttcg tcatcactca 26177 gcatcgacgt cgcgtcttgg tcgctctgta ccacgatctt cttcagctct ttgagctgcg 26237 tctccccagc tttgctgaga aatatcccat aggaacgctt gtccggcttg cagcgcacgc 26297 gcacagcaag gccgagcttc tcgagcttgt tcagcaaggg aaccagttgt ggtggttcga 26357 ttgcgagcat ccgcgctagg tcagcctgca taagcccagg gctcgcttcg atgattagaa 26417 gtgccgacag ctgcgccggg cgtaggtcat atggcgtcag ggcttcaatc aggccctgag 26477 cgagcttcag ctgtgagccg gcgtaaggca tagccaatca attgattcag gagcgtatcg 26537 cccggttcta tcagcgggcc gctttcgaaa gtcatggtgt tagccggtag ggtctttttc 26597 ttggccatgc ttgttgcctg aaccttcgtt gacatagggc agaggtgcgt ttgccgcttc 26657 gcttcgcgat gaaccgcatc gagatgctga ggtcaggatt tttccttaac tcgcgtaagc 26717 attctgtcat ttttttggtg gctttgaaca gcctgatgaa aggtggtctc gccctttgag 26777 gccgattctt gggcgcttgg cggcgtcgaa gcgatgctcc actaccgatt aagataatta 26837 aaataaggaa accgcatggt ttcttatgtg aatttgtctg gcatactcca gctcaagggc 26897 aatttttggg ctattggctg agcagttgcc tctatatggt tattcagaat aacaattgac 26957 tcctcaggag gtcagcgatg agcattcttg gtttgaatgg tgccccggtc ggagctgagc 27017 agctgggctc ggctcttgat cgcatgaaga aggcgcacct ggagcagggg cctgcaaact 27077 tggagctgcg tctgagtagg ctggatcgtg cgattgcaat gcttctggaa aatcgtgaag 27137 caattgccga cgcggtttct gctgactttg gcaatcgcag ccgtgagcaa acactgcttt 27197 gcgacattgc tggctcggtg gcaagcctga aggatagccg cgagcacgtg gccaaatgga 27257 tggagcccga acatcacaag gcgatgtttc caggggcgga ggcacgcgtt gagtttcagc 27317 cgctgggtgt cgttggggtc attagtccct ggaacttccc tatcgtactg gcctttgggc 27377 cgctggccgg catattcgca gcaggtaatc gcgccatgct caagccgtcc gagcttaccc 27437 cgcggacttc tgccctgctt gcggagctaa ttgctcgtta cttcgatgaa actgagctga 27497 ctacagtgct gggcgacgct gaagtcggtg cgctgttcag tgctcagcct ttcgatcatc 27557 tgatcttcac cggcggcact gccgtggcca agcacatcat gcgtgccgcg gcggataacc 27617 tagtgcccgt taccctggaa ttgggtggca aatcgccggt gatcgtttcc cgcagtgcag 27677 atatggcgga cgttgcacaa cgggtgttga cggtgaaaac cttcaatgcc gggcaaatct 27737 gtctggcacc ggactatgtg ctgctgccgg aagaatcgct ggatagcttt gtcgccgagg 27797 cgacgcgctt cgtggccgca atgtatccct cgcttctaga taatccggat tacacgtcga 27857 tcatcaatgc ccgaaatttc gaccgtctgc atcgctacct gactgatgcg caggcaaagg 27917 gagggcgcgt cattgaaatc aatcctgcgg ccgaagagtt gggggatagt ggtatcagga 27977 agatcgcgcc cactttgatc gtgaatgtgt cggatgaaat gctggtcttg aacgaggaga 28037 tctttggtcc gctgctcccg atcaagactt atcgtgattt cgactcggct atcgactacg 28097 tcaacagcaa gcagcgacca cttgcctcgt acttcttcgg cgaagatgcg gttgagcgtg 28157 agcaagtgct taagcgtacg gtttcgggcg ccgtggtcgt gaacgatgtc atgagccatg 28217 tgatgatgga tacgcttcca tttggtggtg tggggcactc ggggatgggg gcatatcacg 28277 gcatttatgg tttccgaacc ttcagccatg ccaagcctgt tctcgtgcaa agtcctgtgg 28337 gtgagtcgaa cttggcgatg cgcgcaccct acggagaagc gatccacgga ctgctctctg 28397 tcctcctttc aacggagtgt tagaaccgtt ggtagtggtt ttggacgggc ccaggagcat 28457 gcgcttctgg gcccgtttct tgagtattca ttggatagtc acgcgtggta gcttcgagcc 28517 tgcacagctg atgagcaccc tggaaggcgc gctgtacgcg gacgactggg ttcatcttcg 28577 ccattcatga cggaactccg ttccccagta ccgcgatgac tattttgcct cttccgatgt 28637 ccgattccac gccgcctgac gctaagcggg ggcgggggcg cccgcatccc agcccagaca 28697 gcaacaaatg agtaggctct tggatgccgc ggcggctgag attggtaacg gcaatttcgt 28757 caatgtgacg atggattcga ttgcccgtgc tgccggcgtc tcaaaaaaaa cgctgtacgt 28817 cttggtggcg agcaaggaag aactcatttc ccggttagtg gctcgagaca tgtccaacct 28877 tgagctgctg ctttgtcacg aggttgagtc tgcggaggcc cttcaggatg agttgcgaaa 28937 ctatctgctg ctctgggcgc gcttgacctt gtcccctctt gctttgggca tttttctgat 28997 ggccgtgcag gggcgtgaaa gtgccccggg cctggcgaga atctggtatc gagagggggc 29057 agagcgttgc ctcagcttgc ttcggggatg gttggcaagg atggcaagcc gggagctgat 29117 cgctcctgga gatatcgact ccgcagtgga gcttatcgat tcgctcctga tctcacagcc 29177 tttgaaatta tttggcctgg ggatccagag cggctggacc gatgatcaga tcaatcaacg 29237 ggtcacaatc gctctcgatg cattccgtcg gtgctatgtc gtttagcacc gttctcgcgg 29297 gctgtggcgg cgtgacctat ttgtctagtg gtcggcgcga aattcgataa gaaagctggg 29357 cgcgagtgag gccgagccgg cgggcagctt ccgagacatt gcctttcacc tggcccagag 29417 catggctaat catcgcgtcc tccacttctt gcagcgtcat cgcgctcagg tcctttgagt 29477 caagcggcga gtcgattgtg ctggtcggtt tggagaagga agtacttggg ctgccagttt 29537 cctgtggctg attatcttga gcggtggcca ggatgccgct ggccccaatg gagaacatcg 29597 gttgagtcag tcgttcaccg ctagtgaaga ggtggctcac gtcaatggct ccatcctccg 29657 gagcgctgat gactccgcgc tccaccaaat tttgaagctc ccggatgttt cctggaaagt 29717 cgtagccaag cagggcattg gctgcacgtg gagtgaatcc gctgaccacc cggctatgac 29777 gctgattgaa gcggtgcagg aaataggtca tcaggagggg aatgtcttcc ttcctctctc 29837 gaagcggcgg gaggtggatc gggtaaacat tgaggcggaa aaaaaggtcc tcgcggaact 29897 cgccgcgctg gacgcctgcg cgaagatcga cattggttgc ggctaccaca cggacgtcaa 29957 ccttgagtgt cctgcttccg ccaacccgtt cgacctccga ctcttgcagg gcgcgaagta 30017 acttcccttg ggccacgagg cttagcgtcc ctatctcgtc aaggaatagt gtgccgcccg 30077 aagcgcgctc gaaccgtcct gctcgagatt gggtggcgcc ggtaaacgcc ccccgttcga 30137 cgccgaacaa ctcggactcc atcagggttt cgggaatacg tgcgcaattg accgcaacaa 30197 acgggccgtc gtgtctgggg ctgatgcggt gaagcatgcg ggcgaacatc tccttgccca 30257 cacctgattc acccgtaaac agtaccgtcg cctccgtggg tgctacgcgc ttcagcatgt 30317 ggcaggcagc attgaatgcc gaggaaattc ccaccatgtc gtgttccgat gcagtgcttg 30377 agtctgcggc ggagtgatgg ggagtgttcc tttgtccctg ctgcgttctt cgtctctgcg 30437 gcgtgcttgg ttgccgacaa atggttgcgc taagcgccgc caagtcctct tcggcgtctt 30497 cccattcttc cgctggcttg ccgatcatgc ggcagatctg cgaacccgtg gagcggcatt 30557 ccacctctcg gtaaaggatg aggcgaccaa ccagcgcgga cgtatagcca atggcataac 30617 ccgtctgcgt ccagcacgcg ggctcggtgc cgatgccgta gtgcgcaata tgttcatcat 30677 cttcgctcga atggtgccag aggaattcgc cgtagtaggt ccccaaatcc atgtcgaagt 30737 cgaagtggat cggctccacg cgtactgcgc cttccagaga gtgcaagttc gggccggcgg 30797 caaataggga gagcggatcg gcgttgctga agcgctcctt cagaagggcg gcatctttgg 30857 cgccgcagtg gtaaccggtt cgcagcatga ttccgcgggc gcgggcgaag cccacgcttt 30917 caattaattc gcgtcgcaat gcacccagtc cgctgctgtg gaggagcagc attcgcgcgc 30977 cgttcaacca gatgcgtcca tcgccagggc tgaaaaggag ggattcagtg aggtcatgaa 31037 gggaggggac ggcgcctggc tccaattgct cgatggcgcc gcgattgagt gtcttgggcg 31097 cggtcttgga gagttcggct agggagataa atttgctggc catggtggcg gcccctgatg 31157 ggttggatga ttttctgcat tctgcatcat gaaattcatg aaatcatcac ttttcggggg 31217 gtgggtgcac gggattgaag gttgctagga gagtgcattg ctcgtaagcc caggaagcac 31277 gcgggtttca ggatggtgca tggaaatggc atgagctttg ctggatatga ttagagacat 31337 taactatttt ggcggaatgg aagcacgatt cctcgcccgg tagagcggta accgcgacat 31397 tcaggaccgt aaaaaggaaa gagcatgcaa ctgaccaaca agaaaatcgt cgtcaccgga 31457 gtgtcctccg gtatcggtgc cgaaactgcc cgcgttctgc gctctcacgg cgccacagtg 31517 attggcgtag atcgcaacat gccgagcctg actctggatg ctttcgttca ggctgacctg 31577 agccatcctg aaggcatcga taaggccatc tctcagctgc cggagaaaat tgacggactc 31637 tgcaatatcg ccggggtgcc cggcactgcc gatcctcagc tcgtcgcaaa cgtgaactac 31697 ctgggtctaa agtatctgac cgaggcagtc ctgtcgcgca ttcaacccgg tggttcgatt 31757 gtcaacgtgt cctctgtgct tggcgccgag tggccggccc gccttcagtt gcataaggag 31817 ctggggagtg ttgttggatt ctccgaaggc caggcatggc ttaagcagaa tccagtggcc 31877 cccgaattct gctaccagta tttcaaagaa gcactgatcg tttggtctca agttcaggcg 31937 caggaatggt tcatgaggac gtctgtacgc atgaactgca tcgcccccgg ccctgtattc 31997 actcccattc tcaatgagtt cgtcaccatg ctgggtcaag agcggactca ggcggacgct 32057 catcgtatta agcgcccagc atatgccgat gaagtggccg cggtgattgc attcatgtgt 32117 gctgaggagt cacgttggat caacggcata aatattccag tggacggagg tttggcatcg 32177 acctacgtgt aagttcgtgg acgccctttg cacgcgcact atatctctat gcagcagctg 32237 aaagcagctt tggttttgat cggaggtagc gggcggaaag gtgcagaatg tctaaataat 32297 aaaggattct tgtgaagctt tagttgtccg taaacgaaaa taaaaataaa gaggaatgat 32357 atgaaagcaa gtagatcagt ctgcactttc aaaatagcta ccctggcagg cgccatttat 32417 gcagcgctgc caatgtcagc tgcaaactcg atgcagctgg atgtaggtag ctcggattgg 32477 acggtgcgtt ggggacaaca ccctcaagta tagccttgcc tctcgcctga atgagcaaga 32537 ctcaagtctg acaaatgcgc cgactgtcaa tggttatatc cggatattca aagtcagggt 32597 gatcgtaact ttgaccgggg gcttggtatc caatcgtctc gatattctgt cggagcttga 32657 tgtcagtcgt gactggttgg tg 32679 2 284 PRT Pseudomonas sp. 2 Met Ile Ala Ile Thr Gly Ala Ser Gly Gln Leu Gly Arg Leu Thr Ile 1 5 10 15 Glu Ala Leu Leu Lys Arg Leu Pro Ala Ser Glu Ile Ile Ala Leu Val 20 25 30 Arg Asp Pro Asn Lys Ala Gly Asp Leu Thr Ala Arg Gly Ile Val Val 35 40 45 Arg Gln Ala Asp Tyr Asn Arg Pro Glu Thr Leu His Arg Ala Leu Ile 50 55 60 Gly Val Asn Arg Leu Leu Leu Ile Ser Ser Ser Glu Val Gly Gln Arg 65 70 75 80 Thr Ala Gln His Arg Ala Val Ile Asp Ala Ala Lys Gln Glu Gly Ile 85 90 95 Glu Leu Leu Ala Tyr Thr Ser Leu Leu His Ala Asp Lys Ser Ala Leu 100 105 110 Gly Leu Ala Thr Glu His Arg Asp Thr Glu Gln Ala Leu Thr Glu Ser 115 120 125 Gly Ile Pro His Val Leu Leu Arg Asn Gly Trp Tyr His Glu Asn Tyr 130 135 140 Thr Ala Gly Ile Pro Val Ala Leu Val His Gly Val Leu Leu Gly Cys 145 150 155 160 Ala Gln Asp Gly Leu Ile Ala Ser Ala Ala Arg Ala Asp Tyr Ala Glu 165 170 175 Ala Ala Ala Val Val Leu Thr Gly Glu Asn Gln Ala Gly Arg Val Tyr 180 185 190 Glu Leu Ala Gly Glu Pro Ala Tyr Thr Leu Thr Glu Leu Ala Ala Glu 195 200 205 Val Ala Pro Gln Ala Gly Lys Thr Val Val Tyr Ser Asn Leu Ser Glu 210 215 220 Ser Asp Tyr Arg Ser Ala Leu Ile Ser Ala Gly Leu Pro Asp Gly Phe 225 230 235 240 Ala Ala Leu Leu Ala Asp Ser Asp Ala Gly Ala Ala Lys Gly Tyr Leu 245 250 255 Phe Asp Ser Ser Gly Asp Ser Arg Lys Leu Ile Gly Arg Pro Thr Thr 260 265 270 Pro Met Ser Glu Ala Ile Ala Ala Ala Ile Gly Arg 275 280 3 1065 DNA Pseudomonas sp. CDS (1)..(1062) product = “Vanillinsaeure-O-Demethylase” / gene =“vanA” 3 atg ttt ccg aaa aac gcc tgg tat gtc gct tgc act ccg gat gaa atc 48 Met Phe Pro Lys Asn Ala Trp Tyr Val Ala Cys Thr Pro Asp Glu Ile 1 5 10 15 gca gat aag ccg cta ggc cgt cag atc tgc aac gaa aag att gtc ttc 96 Ala Asp Lys Pro Leu Gly Arg Gln Ile Cys Asn Glu Lys Ile Val Phe 20 25 30 tat cgg ggg ccg gaa gga cgt gtt gcc gcg gta gag gat ttc tgc cct 144 Tyr Arg Gly Pro Glu Gly Arg Val Ala Ala Val Glu Asp Phe Cys Pro 35 40 45 cat cgc ggg gca ccg ttg tcc ctg ggt ttc gtt cgc gac ggt aag ctg 192 His Arg Gly Ala Pro Leu Ser Leu Gly Phe Val Arg Asp Gly Lys Leu 50 55 60 att tgc ggc tac cac ggt ttg gaa atg ggc tgc gag ggc aaa acg ctc 240 Ile Cys Gly Tyr His Gly Leu Glu Met Gly Cys Glu Gly Lys Thr Leu 65 70 75 80 gcg atg ccc ggg cag cgc gtt caa ggc ttc cct tgc atc aaa agc tac 288 Ala Met Pro Gly Gln Arg Val Gln Gly Phe Pro Cys Ile Lys Ser Tyr 85 90 95 gcg gta gaa gag cga tac ggc ttt atc tgg gta tgg cct ggt gat cgc 336 Ala Val Glu Glu Arg Tyr Gly Phe Ile Trp Val Trp Pro Gly Asp Arg 100 105 110 gag ctg gcg gat ccg gcg ctt att cac cac ctg gag tgg gcc gat aat 384 Glu Leu Ala Asp Pro Ala Leu Ile His His Leu Glu Trp Ala Asp Asn 115 120 125 ccg gag tgg gcc tat ggt ggc ggt ctc tac cac atc gct tgt gat tac 432 Pro Glu Trp Ala Tyr Gly Gly Gly Leu Tyr His Ile Ala Cys Asp Tyr 130 135 140 cgc ctg atg atc gac aac ctc atg gat ctc acc cat gag acc tat gtg 480 Arg Leu Met Ile Asp Asn Leu Met Asp Leu Thr His Glu Thr Tyr Val 145 150 155 160 cat gcc tcc agc atc ggt caa aag gaa att gac gag gca ccg gtc agt 528 His Ala Ser Ser Ile Gly Gln Lys Glu Ile Asp Glu Ala Pro Val Ser 165 170 175 act cgt gtc gag ggc gac acc gtg att acc agc cgg tac atg gat aac 576 Thr Arg Val Glu Gly Asp Thr Val Ile Thr Ser Arg Tyr Met Asp Asn 180 185 190 gtc atg gcc cct ccg ttc tgg cgt gct gcg ctt cgt ggc aac ggc ttg 624 Val Met Ala Pro Pro Phe Trp Arg Ala Ala Leu Arg Gly Asn Gly Leu 195 200 205 gcc gac gat gta ccg gtt gat cgc tgg cag atc tgc cga ttc gct cct 672 Ala Asp Asp Val Pro Val Asp Arg Trp Gln Ile Cys Arg Phe Ala Pro 210 215 220 ccg agt cac gta ctg atc gaa gta ggt gtg gct cat gcg ggc aaa ggc 720 Pro Ser His Val Leu Ile Glu Val Gly Val Ala His Ala Gly Lys Gly 225 230 235 240 gga tat gac gcg ccg gcg gaa tac aag gcc ggc agc ata gtg gtc gac 768 Gly Tyr Asp Ala Pro Ala Glu Tyr Lys Ala Gly Ser Ile Val Val Asp 245 250 255 ttc atc acg ccg gag agt gat acc tcg att tgg tac ttc tgg ggc atg 816 Phe Ile Thr Pro Glu Ser Asp Thr Ser Ile Trp Tyr Phe Trp Gly Met 260 265 270 gct cgc aac ttc cgt ccg cag ggc acg gag ctg act gaa acc att cgt 864 Ala Arg Asn Phe Arg Pro Gln Gly Thr Glu Leu Thr Glu Thr Ile Arg 275 280 285 gtt ggt cag ggc aag att ttt gcc gag gac ctg gac atg ctg gag cag 912 Val Gly Gln Gly Lys Ile Phe Ala Glu Asp Leu Asp Met Leu Glu Gln 290 295 300 cag cag cgc aat ctg ctg gcc tac ccg gag cgc cag ttg ctc aag ctg 960 Gln Gln Arg Asn Leu Leu Ala Tyr Pro Glu Arg Gln Leu Leu Lys Leu 305 310 315 320 aat atc gat gcc ggc ggg gtt cag tca cgg cgc gtc att gat cgg att 1008 Asn Ile Asp Ala Gly Gly Val Gln Ser Arg Arg Val Ile Asp Arg Ile 325 330 335 ctc gca gct gaa caa gag gcc gca gac gca gcg ctg atc gcg aga agt 1056 Leu Ala Ala Glu Gln Glu Ala Ala Asp Ala Ala Leu Ile Ala Arg Ser 340 345 350 gca tca tga 1065 Ala Ser 4 354 PRT Pseudomonas sp. 4 Met Phe Pro Lys Asn Ala Trp Tyr Val Ala Cys Thr Pro Asp Glu Ile 1 5 10 15 Ala Asp Lys Pro Leu Gly Arg Gln Ile Cys Asn Glu Lys Ile Val Phe 20 25 30 Tyr Arg Gly Pro Glu Gly Arg Val Ala Ala Val Glu Asp Phe Cys Pro 35 40 45 His Arg Gly Ala Pro Leu Ser Leu Gly Phe Val Arg Asp Gly Lys Leu 50 55 60 Ile Cys Gly Tyr His Gly Leu Glu Met Gly Cys Glu Gly Lys Thr Leu 65 70 75 80 Ala Met Pro Gly Gln Arg Val Gln Gly Phe Pro Cys Ile Lys Ser Tyr 85 90 95 Ala Val Glu Glu Arg Tyr Gly Phe Ile Trp Val Trp Pro Gly Asp Arg 100 105 110 Glu Leu Ala Asp Pro Ala Leu Ile His His Leu Glu Trp Ala Asp Asn 115 120 125 Pro Glu Trp Ala Tyr Gly Gly Gly Leu Tyr His Ile Ala Cys Asp Tyr 130 135 140 Arg Leu Met Ile Asp Asn Leu Met Asp Leu Thr His Glu Thr Tyr Val 145 150 155 160 His Ala Ser Ser Ile Gly Gln Lys Glu Ile Asp Glu Ala Pro Val Ser 165 170 175 Thr Arg Val Glu Gly Asp Thr Val Ile Thr Ser Arg Tyr Met Asp Asn 180 185 190 Val Met Ala Pro Pro Phe Trp Arg Ala Ala Leu Arg Gly Asn Gly Leu 195 200 205 Ala Asp Asp Val Pro Val Asp Arg Trp Gln Ile Cys Arg Phe Ala Pro 210 215 220 Pro Ser His Val Leu Ile Glu Val Gly Val Ala His Ala Gly Lys Gly 225 230 235 240 Gly Tyr Asp Ala Pro Ala Glu Tyr Lys Ala Gly Ser Ile Val Val Asp 245 250 255 Phe Ile Thr Pro Glu Ser Asp Thr Ser Ile Trp Tyr Phe Trp Gly Met 260 265 270 Ala Arg Asn Phe Arg Pro Gln Gly Thr Glu Leu Thr Glu Thr Ile Arg 275 280 285 Val Gly Gln Gly Lys Ile Phe Ala Glu Asp Leu Asp Met Leu Glu Gln 290 295 300 Gln Gln Arg Asn Leu Leu Ala Tyr Pro Glu Arg Gln Leu Leu Lys Leu 305 310 315 320 Asn Ile Asp Ala Gly Gly Val Gln Ser Arg Arg Val Ile Asp Arg Ile 325 330 335 Leu Ala Ala Glu Gln Glu Ala Ala Asp Ala Ala Leu Ile Ala Arg Ser 340 345 350 Ala Ser 5 954 DNA Pseudomonas sp. CDS (1)..(951) product = “Vanillin-O-Demethylase”/ gene = “vanB” 5 atg att gag gta atc att tcg gcg atg cgc ttg gtt gct cag gac atc 48 Met Ile Glu Val Ile Ile Ser Ala Met Arg Leu Val Ala Gln Asp Ile 1 5 10 15 att agc ctt gag ttt gtc cgg gct gac ggt ggc ttg ctt ccg cct gtc 96 Ile Ser Leu Glu Phe Val Arg Ala Asp Gly Gly Leu Leu Pro Pro Val 20 25 30 gag gcc ggc gcc cac gtc gat gtg cat ctt cct ggc ggc ctg att cgg 144 Glu Ala Gly Ala His Val Asp Val His Leu Pro Gly Gly Leu Ile Arg 35 40 45 cag tac tcg ctc tgg aat caa cca ggg gcg cag agc cat tac tgc atc 192 Gln Tyr Ser Leu Trp Asn Gln Pro Gly Ala Gln Ser His Tyr Cys Ile 50 55 60 ggt gtt ctg aag gac ccg gcg tct cgt ggt ggt tcg aag gcg gtg cac 240 Gly Val Leu Lys Asp Pro Ala Ser Arg Gly Gly Ser Lys Ala Val His 65 70 75 80 gag aat ctt cgc gtc ggg atg cgc gtg caa att agc gag ccg agg aac 288 Glu Asn Leu Arg Val Gly Met Arg Val Gln Ile Ser Glu Pro Arg Asn 85 90 95 cta ttc cca ttg gaa gag ggg gtg gag cgg agt ctg ctg ttc gcg ggc 336 Leu Phe Pro Leu Glu Glu Gly Val Glu Arg Ser Leu Leu Phe Ala Gly 100 105 110 ggg att ggc att acg ccg att ctg tgt atg gct caa gaa tta gca gca 384 Gly Ile Gly Ile Thr Pro Ile Leu Cys Met Ala Gln Glu Leu Ala Ala 115 120 125 cgc gag caa gat ttc gag ttg cat tat tgc gcg cgt tcg acc gac cga 432 Arg Glu Gln Asp Phe Glu Leu His Tyr Cys Ala Arg Ser Thr Asp Arg 130 135 140 gcg gcg ttc gtt gaa tgg ctt aag gtt tgc gac ttt gct gat cac gta 480 Ala Ala Phe Val Glu Trp Leu Lys Val Cys Asp Phe Ala Asp His Val 145 150 155 160 cgt ttc cac ttt gac aat ggc ccg gat cag caa aaa ctg aat gcc gca 528 Arg Phe His Phe Asp Asn Gly Pro Asp Gln Gln Lys Leu Asn Ala Ala 165 170 175 gcg ctg cta gcg gcc gag gcc gaa ggt acc cac ctt tat gtc tgt ggg 576 Ala Leu Leu Ala Ala Glu Ala Glu Gly Thr His Leu Tyr Val Cys Gly 180 185 190 ccc ggc ggg ttc atg ggg cat gtg ctt gat acc gcg aag gag cag ggc 624 Pro Gly Gly Phe Met Gly His Val Leu Asp Thr Ala Lys Glu Gln Gly 195 200 205 tgg gct gac aat cga ctg cat cga gag tat ttc gcc gcg gcg ccg aat 672 Trp Ala Asp Asn Arg Leu His Arg Glu Tyr Phe Ala Ala Ala Pro Asn 210 215 220 gtg agt gct gac gat ggc agt ttc gag gtg cgg att cac agc acc gga 720 Val Ser Ala Asp Asp Gly Ser Phe Glu Val Arg Ile His Ser Thr Gly 225 230 235 240 caa gtg ctt cag gtc ccc gcg gat caa acg gtc tcc cag gtg ctc gat 768 Gln Val Leu Gln Val Pro Ala Asp Gln Thr Val Ser Gln Val Leu Asp 245 250 255 gcg gcc gga att atc gtt ccc gtt tct tgt gag cag ggc atc tgc ggt 816 Ala Ala Gly Ile Ile Val Pro Val Ser Cys Glu Gln Gly Ile Cys Gly 260 265 270 act tgc atc act cgg gtg gta gac gga gag cct gat cat cgt gac ttc 864 Thr Cys Ile Thr Arg Val Val Asp Gly Glu Pro Asp His Arg Asp Phe 275 280 285 ttc ctc acg gat gcg gag aag gca aag aac gac cag ttc acc ccc tgt 912 Phe Leu Thr Asp Ala Glu Lys Ala Lys Asn Asp Gln Phe Thr Pro Cys 290 295 300 tgc tcg cga gcc aag agc gcc tgt ttg gtc ttg gat ctc taa 954 Cys Ser Arg Ala Lys Ser Ala Cys Leu Val Leu Asp Leu 305 310 315 6 317 PRT Pseudomonas sp. 6 Met Ile Glu Val Ile Ile Ser Ala Met Arg Leu Val Ala Gln Asp Ile 1 5 10 15 Ile Ser Leu Glu Phe Val Arg Ala Asp Gly Gly Leu Leu Pro Pro Val 20 25 30 Glu Ala Gly Ala His Val Asp Val His Leu Pro Gly Gly Leu Ile Arg 35 40 45 Gln Tyr Ser Leu Trp Asn Gln Pro Gly Ala Gln Ser His Tyr Cys Ile 50 55 60 Gly Val Leu Lys Asp Pro Ala Ser Arg Gly Gly Ser Lys Ala Val His 65 70 75 80 Glu Asn Leu Arg Val Gly Met Arg Val Gln Ile Ser Glu Pro Arg Asn 85 90 95 Leu Phe Pro Leu Glu Glu Gly Val Glu Arg Ser Leu Leu Phe Ala Gly 100 105 110 Gly Ile Gly Ile Thr Pro Ile Leu Cys Met Ala Gln Glu Leu Ala Ala 115 120 125 Arg Glu Gln Asp Phe Glu Leu His Tyr Cys Ala Arg Ser Thr Asp Arg 130 135 140 Ala Ala Phe Val Glu Trp Leu Lys Val Cys Asp Phe Ala Asp His Val 145 150 155 160 Arg Phe His Phe Asp Asn Gly Pro Asp Gln Gln Lys Leu Asn Ala Ala 165 170 175 Ala Leu Leu Ala Ala Glu Ala Glu Gly Thr His Leu Tyr Val Cys Gly 180 185 190 Pro Gly Gly Phe Met Gly His Val Leu Asp Thr Ala Lys Glu Gln Gly 195 200 205 Trp Ala Asp Asn Arg Leu His Arg Glu Tyr Phe Ala Ala Ala Pro Asn 210 215 220 Val Ser Ala Asp Asp Gly Ser Phe Glu Val Arg Ile His Ser Thr Gly 225 230 235 240 Gln Val Leu Gln Val Pro Ala Asp Gln Thr Val Ser Gln Val Leu Asp 245 250 255 Ala Ala Gly Ile Ile Val Pro Val Ser Cys Glu Gln Gly Ile Cys Gly 260 265 270 Thr Cys Ile Thr Arg Val Val Asp Gly Glu Pro Asp His Arg Asp Phe 275 280 285 Phe Leu Thr Asp Ala Glu Lys Ala Lys Asn Asp Gln Phe Thr Pro Cys 290 295 300 Cys Ser Arg Ala Lys Ser Ala Cys Leu Val Leu Asp Leu 305 310 315 7 1119 DNA Pseudomonas sp. CDS (1)..(1116) product = “Formaldehyd-Dehydrogenase”/ gene = “fdh” 7 atg atc aaa tcc cgc gcc gct gtg gcg ttc gca ccc aat cag cca ttg 48 Met Ile Lys Ser Arg Ala Ala Val Ala Phe Ala Pro Asn Gln Pro Leu 1 5 10 15 cag atc gtc gaa gtg gac gtg gct ccg ccc aag gcc ggt gaa gtc ctg 96 Gln Ile Val Glu Val Asp Val Ala Pro Pro Lys Ala Gly Glu Val Leu 20 25 30 gtg cgg gtc gtg gcc acc ggc gtt tgc cac acc gat gcc tac acc ctg 144 Val Arg Val Val Ala Thr Gly Val Cys His Thr Asp Ala Tyr Thr Leu 35 40 45 tcc ggc gct gat tcc gag ggc gtt ttc ccc tgc atc ctt ggt cac gaa 192 Ser Gly Ala Asp Ser Glu Gly Val Phe Pro Cys Ile Leu Gly His Glu 50 55 60 ggc ggc ggc att gtc gaa gcg gtg ggc gag ggc gtc acc tcg ctg gcg 240 Gly Gly Gly Ile Val Glu Ala Val Gly Glu Gly Val Thr Ser Leu Ala 65 70 75 80 gtc ggc gac cac gtg atc ccg ctc tac acg gcc gaa tgc cgt gag tgc 288 Val Gly Asp His Val Ile Pro Leu Tyr Thr Ala Glu Cys Arg Glu Cys 85 90 95 aag ttc ttc aag tcc ggc aag acc aac ctg tgc cag aaa gtg cgt gct 336 Lys Phe Phe Lys Ser Gly Lys Thr Asn Leu Cys Gln Lys Val Arg Ala 100 105 110 act cag ggc aag ggt ctg atg ccg gac ggc acc tcc cgc ttc agc tac 384 Thr Gln Gly Lys Gly Leu Met Pro Asp Gly Thr Ser Arg Phe Ser Tyr 115 120 125 aac ggt cag ccg atc tac cac tac atg ggc tgc tcg acc ttc tcc gag 432 Asn Gly Gln Pro Ile Tyr His Tyr Met Gly Cys Ser Thr Phe Ser Glu 130 135 140 tac acc gtg ctg ccg gaa atc tcc ctg gcg aag att ccc aag aat gcg 480 Tyr Thr Val Leu Pro Glu Ile Ser Leu Ala Lys Ile Pro Lys Asn Ala 145 150 155 160 ccg ctg gag aaa gtc tgc ctg ctg ggc tgc ggc gtg acc acc ggc att 528 Pro Leu Glu Lys Val Cys Leu Leu Gly Cys Gly Val Thr Thr Gly Ile 165 170 175 ggc gcg gtg ctg aac act gcc aag gtg gag gag ggt gct acc gtg gcc 576 Gly Ala Val Leu Asn Thr Ala Lys Val Glu Glu Gly Ala Thr Val Ala 180 185 190 atc ttc ggc ctg ggc ggc atc ggc ttg gcg gcg atc atc ggc gcg aag 624 Ile Phe Gly Leu Gly Gly Ile Gly Leu Ala Ala Ile Ile Gly Ala Lys 195 200 205 atg gcc aag gcc tcg cgc atc atc gcc atc gac atc aat ccg tcc aag 672 Met Ala Lys Ala Ser Arg Ile Ile Ala Ile Asp Ile Asn Pro Ser Lys 210 215 220 ttc gat gtg gct cgc gag ctg ggc gcc act gac ttc gtc aat ccg aac 720 Phe Asp Val Ala Arg Glu Leu Gly Ala Thr Asp Phe Val Asn Pro Asn 225 230 235 240 gat cac gcg aag ccg atc cag gat gtc atc gtc gag atg act gat ggc 768 Asp His Ala Lys Pro Ile Gln Asp Val Ile Val Glu Met Thr Asp Gly 245 250 255 ggt gtg gac tac agc ttc gag tgc atc ggc aac gtt cga ctc atg cgc 816 Gly Val Asp Tyr Ser Phe Glu Cys Ile Gly Asn Val Arg Leu Met Arg 260 265 270 gca gca ctc gag tgc tgc cac aag ggc tgg ggc gaa tcc gtg atc atc 864 Ala Ala Leu Glu Cys Cys His Lys Gly Trp Gly Glu Ser Val Ile Ile 275 280 285 ggc gtg gcg ccg gcg ggg gcc gaa atc aac acc cgt ccg ttc cac ctg 912 Gly Val Ala Pro Ala Gly Ala Glu Ile Asn Thr Arg Pro Phe His Leu 290 295 300 gtg acc ggt cgc gtc tgg cgg ggt tcg gcg ttc ggt ggc gta aag ggc 960 Val Thr Gly Arg Val Trp Arg Gly Ser Ala Phe Gly Gly Val Lys Gly 305 310 315 320 cgc acc gaa ctg ccg agc tac gtg gag aag gca cag cag ggc gag atc 1008 Arg Thr Glu Leu Pro Ser Tyr Val Glu Lys Ala Gln Gln Gly Glu Ile 325 330 335 ccg ctg gac acc ttc atc act cac acc atg ggc ctg gac gac atc aac 1056 Pro Leu Asp Thr Phe Ile Thr His Thr Met Gly Leu Asp Asp Ile Asn 340 345 350 acg gcc ttc gac ctg atg gac gaa ggg aag agc atc cgc tct gtt gtt 1104 Thr Ala Phe Asp Leu Met Asp Glu Gly Lys Ser Ile Arg Ser Val Val 355 360 365 caa ttg agt cgc tag 1119 Gln Leu Ser Arg 370 8 372 PRT Pseudomonas sp. 8 Met Ile Lys Ser Arg Ala Ala Val Ala Phe Ala Pro Asn Gln Pro Leu 1 5 10 15 Gln Ile Val Glu Val Asp Val Ala Pro Pro Lys Ala Gly Glu Val Leu 20 25 30 Val Arg Val Val Ala Thr Gly Val Cys His Thr Asp Ala Tyr Thr Leu 35 40 45 Ser Gly Ala Asp Ser Glu Gly Val Phe Pro Cys Ile Leu Gly His Glu 50 55 60 Gly Gly Gly Ile Val Glu Ala Val Gly Glu Gly Val Thr Ser Leu Ala 65 70 75 80 Val Gly Asp His Val Ile Pro Leu Tyr Thr Ala Glu Cys Arg Glu Cys 85 90 95 Lys Phe Phe Lys Ser Gly Lys Thr Asn Leu Cys Gln Lys Val Arg Ala 100 105 110 Thr Gln Gly Lys Gly Leu Met Pro Asp Gly Thr Ser Arg Phe Ser Tyr 115 120 125 Asn Gly Gln Pro Ile Tyr His Tyr Met Gly Cys Ser Thr Phe Ser Glu 130 135 140 Tyr Thr Val Leu Pro Glu Ile Ser Leu Ala Lys Ile Pro Lys Asn Ala 145 150 155 160 Pro Leu Glu Lys Val Cys Leu Leu Gly Cys Gly Val Thr Thr Gly Ile 165 170 175 Gly Ala Val Leu Asn Thr Ala Lys Val Glu Glu Gly Ala Thr Val Ala 180 185 190 Ile Phe Gly Leu Gly Gly Ile Gly Leu Ala Ala Ile Ile Gly Ala Lys 195 200 205 Met Ala Lys Ala Ser Arg Ile Ile Ala Ile Asp Ile Asn Pro Ser Lys 210 215 220 Phe Asp Val Ala Arg Glu Leu Gly Ala Thr Asp Phe Val Asn Pro Asn 225 230 235 240 Asp His Ala Lys Pro Ile Gln Asp Val Ile Val Glu Met Thr Asp Gly 245 250 255 Gly Val Asp Tyr Ser Phe Glu Cys Ile Gly Asn Val Arg Leu Met Arg 260 265 270 Ala Ala Leu Glu Cys Cys His Lys Gly Trp Gly Glu Ser Val Ile Ile 275 280 285 Gly Val Ala Pro Ala Gly Ala Glu Ile Asn Thr Arg Pro Phe His Leu 290 295 300 Val Thr Gly Arg Val Trp Arg Gly Ser Ala Phe Gly Gly Val Lys Gly 305 310 315 320 Arg Thr Glu Leu Pro Ser Tyr Val Glu Lys Ala Gln Gln Gly Glu Ile 325 330 335 Pro Leu Asp Thr Phe Ile Thr His Thr Met Gly Leu Asp Asp Ile Asn 340 345 350 Thr Ala Phe Asp Leu Met Asp Glu Gly Lys Ser Ile Arg Ser Val Val 355 360 365 Gln Leu Ser Arg 370 9 1638 DNA Pseudomonas sp. CDS (1)..(1635) product = “gamma-Glutamylcystein-Synthetase” / gene = “gcs” 9 atg ccg caa act ctt gct gga cgg ttg agt ctg tta tcc ggc acc gac 48 Met Pro Gln Thr Leu Ala Gly Arg Leu Ser Leu Leu Ser Gly Thr Asp 1 5 10 15 gaa tta acc ctg ctt ctt cgg ggt ggt cgg ggc att gag cgt gaa gcc 96 Glu Leu Thr Leu Leu Leu Arg Gly Gly Arg Gly Ile Glu Arg Glu Ala 20 25 30 ttg cgg gtc gat gtt caa ggt gaa ctg gcg ctg acg cct cac ccg gcg 144 Leu Arg Val Asp Val Gln Gly Glu Leu Ala Leu Thr Pro His Pro Ala 35 40 45 gcg ctt ggc tct gcg ttg acc cat ccg aca att act acg gat tac gcc 192 Ala Leu Gly Ser Ala Leu Thr His Pro Thr Ile Thr Thr Asp Tyr Ala 50 55 60 gag gcc ctg ctt gag ttg atc act cgg ccg gca acc gat tgt gcg caa 240 Glu Ala Leu Leu Glu Leu Ile Thr Arg Pro Ala Thr Asp Cys Ala Gln 65 70 75 80 gcc ttg gct gag ctg gag gag ctt cac cgt ttc gtt cat tcg aga ctt 288 Ala Leu Ala Glu Leu Glu Glu Leu His Arg Phe Val His Ser Arg Leu 85 90 95 gag ggg gag tat ctc tgg aat ctg tcc atg cct ggc aga ttg ccg gtt 336 Glu Gly Glu Tyr Leu Trp Asn Leu Ser Met Pro Gly Arg Leu Pro Val 100 105 110 gat gag caa atc ccg att gct tgg tat gga cca tca aat cca ggc atg 384 Asp Glu Gln Ile Pro Ile Ala Trp Tyr Gly Pro Ser Asn Pro Gly Met 115 120 125 ttg cgc cac gtt tat cgc cgt ggc cta gct ctg cgt tat ggc aag cga 432 Leu Arg His Val Tyr Arg Arg Gly Leu Ala Leu Arg Tyr Gly Lys Arg 130 135 140 atg caa tgc atc gca ggg att cac tac aac tac tca ctg ccg cca gag 480 Met Gln Cys Ile Ala Gly Ile His Tyr Asn Tyr Ser Leu Pro Pro Glu 145 150 155 160 ctt ttc gct gtc ctg acc aag gca gag gtc ggg tct ccc aag tta ctg 528 Leu Phe Ala Val Leu Thr Lys Ala Glu Val Gly Ser Pro Lys Leu Leu 165 170 175 gag cgc cag tca gca gct tac atg cgc caa att cgc aac ctt cgg caa 576 Glu Arg Gln Ser Ala Ala Tyr Met Arg Gln Ile Arg Asn Leu Arg Gln 180 185 190 tac ggt tgg ttg ctg gcc tac ttg ttc ggc gct tcc ccc gcc atc tgc 624 Tyr Gly Trp Leu Leu Ala Tyr Leu Phe Gly Ala Ser Pro Ala Ile Cys 195 200 205 aag agc ttc ttg ggg ggc gag aga gat gag cta gct cgc atg ggg ggc 672 Lys Ser Phe Leu Gly Gly Glu Arg Asp Glu Leu Ala Arg Met Gly Gly 210 215 220 gat acg ctt tac atg ccc tat gca acc agc ttg cgc atg agt gac atc 720 Asp Thr Leu Tyr Met Pro Tyr Ala Thr Ser Leu Arg Met Ser Asp Ile 225 230 235 240 ggg tac cgc aac cgt gcc atg gat gat cta tct ccc agc ctg aat gat 768 Gly Tyr Arg Asn Arg Ala Met Asp Asp Leu Ser Pro Ser Leu Asn Asp 245 250 255 ctg ggt gcc tat att cgc gat att tgc cgt gct ctt cac act ccc gat 816 Leu Gly Ala Tyr Ile Arg Asp Ile Cys Arg Ala Leu His Thr Pro Asp 260 265 270 gcc cag tac cag gcg ctg ggt gtg ttt gca cag ggc gag tgg cgg cag 864 Ala Gln Tyr Gln Ala Leu Gly Val Phe Ala Gln Gly Glu Trp Arg Gln 275 280 285 tta aac gcc aat cta ttg cag ttg gat agt gag tac tac gca ctg gcg 912 Leu Asn Ala Asn Leu Leu Gln Leu Asp Ser Glu Tyr Tyr Ala Leu Ala 290 295 300 cga ccg aag tca gcg ccc gag cgg ggg gag cga aac ctg gat gct ctc 960 Arg Pro Lys Ser Ala Pro Glu Arg Gly Glu Arg Asn Leu Asp Ala Leu 305 310 315 320 gct agg cgt gga gtc cag tat gtg gag ctg cgc gca ctg gat ctc gat 1008 Ala Arg Arg Gly Val Gln Tyr Val Glu Leu Arg Ala Leu Asp Leu Asp 325 330 335 cca ttc tcc ccg tta ggc att ggc ctg acc tgc gcc aag ttc ctc gat 1056 Pro Phe Ser Pro Leu Gly Ile Gly Leu Thr Cys Ala Lys Phe Leu Asp 340 345 350 ggc ttt ttg ctt ttc tgc ttg ttg tct gag gcg ccg gtt gat gat cga 1104 Gly Phe Leu Leu Phe Cys Leu Leu Ser Glu Ala Pro Val Asp Asp Arg 355 360 365 aat gcc cag cgt tca aga ccg gga aaa tct gag cct ggc cgg caa gta 1152 Asn Ala Gln Arg Ser Arg Pro Gly Lys Ser Glu Pro Gly Arg Gln Val 370 375 380 cgg gcg tca cct ggc tta aag ctg cat cgg aat ggt cag tcc att ctc 1200 Arg Ala Ser Pro Gly Leu Lys Leu His Arg Asn Gly Gln Ser Ile Leu 385 390 395 400 ctc aag gat tgg gcg cag gaa gtg ttg acg gag gtt cag gcc tgt gtg 1248 Leu Lys Asp Trp Ala Gln Glu Val Leu Thr Glu Val Gln Ala Cys Val 405 410 415 gaa ttg ctc gac agt gca aat ggg ggc tca tct cac gca ttg gct tgg 1296 Glu Leu Leu Asp Ser Ala Asn Gly Gly Ser Ser His Ala Leu Ala Trp 420 425 430 tca gca cag gag gaa aag gtg ctt aat ccg gat tgt gcg cca tca gct 1344 Ser Ala Gln Glu Glu Lys Val Leu Asn Pro Asp Cys Ala Pro Ser Ala 435 440 445 cag gtg ctc gca gag ata cac aga cac ggt ggg agc ttc acg gca ttt 1392 Gln Val Leu Ala Glu Ile His Arg His Gly Gly Ser Phe Thr Ala Phe 450 455 460 ggt cgc caa tta gct atc gac cat gca aaa cac ttc agt gcc tcc tcg 1440 Gly Arg Gln Leu Ala Ile Asp His Ala Lys His Phe Ser Ala Ser Ser 465 470 475 480 ctt gag gct ggc gta gcc aaa gcg ctt gac ctc cag gcg acg tcg tct 1488 Leu Glu Ala Gly Val Ala Lys Ala Leu Asp Leu Gln Ala Thr Ser Ser 485 490 495 ctg cgc gag cag cat caa ttg gag gcc aac gac cgt gcg cca ttt tct 1536 Leu Arg Glu Gln His Gln Leu Glu Ala Asn Asp Arg Ala Pro Phe Ser 500 505 510 gac tac ctt cag caa ttc tcc ctg gct ttc ggt caa tcc gtc ggc gcc 1584 Asp Tyr Leu Gln Gln Phe Ser Leu Ala Phe Gly Gln Ser Val Gly Ala 515 520 525 tct cgt gcg ccc aac cct acc gcg cac ctc atc gat ctg acc cct cct 1632 Ser Arg Ala Pro Asn Pro Thr Ala His Leu Ile Asp Leu Thr Pro Pro 530 535 540 gtc taa 1638 Val 545 10 545 PRT Pseudomonas sp. 10 Met Pro Gln Thr Leu Ala Gly Arg Leu Ser Leu Leu Ser Gly Thr Asp 1 5 10 15 Glu Leu Thr Leu Leu Leu Arg Gly Gly Arg Gly Ile Glu Arg Glu Ala 20 25 30 Leu Arg Val Asp Val Gln Gly Glu Leu Ala Leu Thr Pro His Pro Ala 35 40 45 Ala Leu Gly Ser Ala Leu Thr His Pro Thr Ile Thr Thr Asp Tyr Ala 50 55 60 Glu Ala Leu Leu Glu Leu Ile Thr Arg Pro Ala Thr Asp Cys Ala Gln 65 70 75 80 Ala Leu Ala Glu Leu Glu Glu Leu His Arg Phe Val His Ser Arg Leu 85 90 95 Glu Gly Glu Tyr Leu Trp Asn Leu Ser Met Pro Gly Arg Leu Pro Val 100 105 110 Asp Glu Gln Ile Pro Ile Ala Trp Tyr Gly Pro Ser Asn Pro Gly Met 115 120 125 Leu Arg His Val Tyr Arg Arg Gly Leu Ala Leu Arg Tyr Gly Lys Arg 130 135 140 Met Gln Cys Ile Ala Gly Ile His Tyr Asn Tyr Ser Leu Pro Pro Glu 145 150 155 160 Leu Phe Ala Val Leu Thr Lys Ala Glu Val Gly Ser Pro Lys Leu Leu 165 170 175 Glu Arg Gln Ser Ala Ala Tyr Met Arg Gln Ile Arg Asn Leu Arg Gln 180 185 190 Tyr Gly Trp Leu Leu Ala Tyr Leu Phe Gly Ala Ser Pro Ala Ile Cys 195 200 205 Lys Ser Phe Leu Gly Gly Glu Arg Asp Glu Leu Ala Arg Met Gly Gly 210 215 220 Asp Thr Leu Tyr Met Pro Tyr Ala Thr Ser Leu Arg Met Ser Asp Ile 225 230 235 240 Gly Tyr Arg Asn Arg Ala Met Asp Asp Leu Ser Pro Ser Leu Asn Asp 245 250 255 Leu Gly Ala Tyr Ile Arg Asp Ile Cys Arg Ala Leu His Thr Pro Asp 260 265 270 Ala Gln Tyr Gln Ala Leu Gly Val Phe Ala Gln Gly Glu Trp Arg Gln 275 280 285 Leu Asn Ala Asn Leu Leu Gln Leu Asp Ser Glu Tyr Tyr Ala Leu Ala 290 295 300 Arg Pro Lys Ser Ala Pro Glu Arg Gly Glu Arg Asn Leu Asp Ala Leu 305 310 315 320 Ala Arg Arg Gly Val Gln Tyr Val Glu Leu Arg Ala Leu Asp Leu Asp 325 330 335 Pro Phe Ser Pro Leu Gly Ile Gly Leu Thr Cys Ala Lys Phe Leu Asp 340 345 350 Gly Phe Leu Leu Phe Cys Leu Leu Ser Glu Ala Pro Val Asp Asp Arg 355 360 365 Asn Ala Gln Arg Ser Arg Pro Gly Lys Ser Glu Pro Gly Arg Gln Val 370 375 380 Arg Ala Ser Pro Gly Leu Lys Leu His Arg Asn Gly Gln Ser Ile Leu 385 390 395 400 Leu Lys Asp Trp Ala Gln Glu Val Leu Thr Glu Val Gln Ala Cys Val 405 410 415 Glu Leu Leu Asp Ser Ala Asn Gly Gly Ser Ser His Ala Leu Ala Trp 420 425 430 Ser Ala Gln Glu Glu Lys Val Leu Asn Pro Asp Cys Ala Pro Ser Ala 435 440 445 Gln Val Leu Ala Glu Ile His Arg His Gly Gly Ser Phe Thr Ala Phe 450 455 460 Gly Arg Gln Leu Ala Ile Asp His Ala Lys His Phe Ser Ala Ser Ser 465 470 475 480 Leu Glu Ala Gly Val Ala Lys Ala Leu Asp Leu Gln Ala Thr Ser Ser 485 490 495 Leu Arg Glu Gln His Gln Leu Glu Ala Asn Asp Arg Ala Pro Phe Ser 500 505 510 Asp Tyr Leu Gln Gln Phe Ser Leu Ala Phe Gly Gln Ser Val Gly Ala 515 520 525 Ser Arg Ala Pro Asn Pro Thr Ala His Leu Ile Asp Leu Thr Pro Pro 530 535 540 Val 545 11 354 DNA Pseudomonas sp. CDS (1)..(351) product = “Cytochrom C UE-Eugenol-Hydroxylase” /gene = “chyA” 11 atg atg aat gtt aat tat aag gct gtc ggg gcg agc cta ctc ctc gcc 48 Met Met Asn Val Asn Tyr Lys Ala Val Gly Ala Ser Leu Leu Leu Ala 1 5 10 15 ttc atc tct cag gga gct tgg gca gag agc ccc gca gcc tct ggc aat 96 Phe Ile Ser Gln Gly Ala Trp Ala Glu Ser Pro Ala Ala Ser Gly Asn 20 25 30 acc cct gac att tat cga aag acc tgc acc tac tgc cat gag cct act 144 Thr Pro Asp Ile Tyr Arg Lys Thr Cys Thr Tyr Cys His Glu Pro Thr 35 40 45 gtc aac aat ggc cgg gtc att gcc cga agc ctc ggg ccg act ctg cga 192 Val Asn Asn Gly Arg Val Ile Ala Arg Ser Leu Gly Pro Thr Leu Arg 50 55 60 ggg cgc cag atc cct cca cag tac acg gag tac atg gtg cgt cat gga 240 Gly Arg Gln Ile Pro Pro Gln Tyr Thr Glu Tyr Met Val Arg His Gly 65 70 75 80 cgc ggg gca atg cct gca ttc tct gaa gca gaa gtg cct ccg gcg gag 288 Arg Gly Ala Met Pro Ala Phe Ser Glu Ala Glu Val Pro Pro Ala Glu 85 90 95 ctg aaa gtt ctg ggc gat tgg att cag caa agc agt gct ccc aaa gac 336 Leu Lys Val Leu Gly Asp Trp Ile Gln Gln Ser Ser Ala Pro Lys Asp 100 105 110 gct gga gtc gcg cca tga 354 Ala Gly Val Ala Pro 115 12 117 PRT Pseudomonas sp. 12 Met Met Asn Val Asn Tyr Lys Ala Val Gly Ala Ser Leu Leu Leu Ala 1 5 10 15 Phe Ile Ser Gln Gly Ala Trp Ala Glu Ser Pro Ala Ala Ser Gly Asn 20 25 30 Thr Pro Asp Ile Tyr Arg Lys Thr Cys Thr Tyr Cys His Glu Pro Thr 35 40 45 Val Asn Asn Gly Arg Val Ile Ala Arg Ser Leu Gly Pro Thr Leu Arg 50 55 60 Gly Arg Gln Ile Pro Pro Gln Tyr Thr Glu Tyr Met Val Arg His Gly 65 70 75 80 Arg Gly Ala Met Pro Ala Phe Ser Glu Ala Glu Val Pro Pro Ala Glu 85 90 95 Leu Lys Val Leu Gly Asp Trp Ile Gln Gln Ser Ser Ala Pro Lys Asp 100 105 110 Ala Gly Val Ala Pro 115 13 687 DNA Pseudomonas sp. CDS (1)..(684) gene = “ORF5” 13 atg act acc cgt cgc aac ttt cta ata ggc gcg tcg cag gtg ggg gca 48 Met Thr Thr Arg Arg Asn Phe Leu Ile Gly Ala Ser Gln Val Gly Ala 1 5 10 15 ttg gtg atg atg tcg ccg aaa ttg gtc ttc cgt acg ccg ctc aag cag 96 Leu Val Met Met Ser Pro Lys Leu Val Phe Arg Thr Pro Leu Lys Gln 20 25 30 aag ccc gtg cgc atc ctg tcg acc ggg ctg gcc ggt gag caa gag ttt 144 Lys Pro Val Arg Ile Leu Ser Thr Gly Leu Ala Gly Glu Gln Glu Phe 35 40 45 cac tcg atg ctt cgc gcg cga ttg acc cat acg ggt cag gtc gac atc 192 His Ser Met Leu Arg Ala Arg Leu Thr His Thr Gly Gln Val Asp Ile 50 55 60 gcg tcg gta ccg ctg gac gca gct att tgg gct tct ccc gct cga ctt 240 Ala Ser Val Pro Leu Asp Ala Ala Ile Trp Ala Ser Pro Ala Arg Leu 65 70 75 80 gcc cag gca atg gat gcg ttg aat ggt acg cgt ctg atc gct ttt gtt 288 Ala Gln Ala Met Asp Ala Leu Asn Gly Thr Arg Leu Ile Ala Phe Val 85 90 95 gag ccc agg aac gaa ttg ata ctg atg caa ttc ttg atg gat cgc ggg 336 Glu Pro Arg Asn Glu Leu Ile Leu Met Gln Phe Leu Met Asp Arg Gly 100 105 110 gct gcg gtg ctt att caa ggt gag cat gcg gtg gac agc aag ggg gtc 384 Ala Ala Val Leu Ile Gln Gly Glu His Ala Val Asp Ser Lys Gly Val 115 120 125 tct cgg cac gac ttt ctg agt acc cca tcc agt gcg gga att gga ggg 432 Ser Arg His Asp Phe Leu Ser Thr Pro Ser Ser Ala Gly Ile Gly Gly 130 135 140 gcg cta gcc gac agc ctg gca aaa ggg ggc tcg ccg ttc tct att tcc 480 Ala Leu Ala Asp Ser Leu Ala Lys Gly Gly Ser Pro Phe Ser Ile Ser 145 150 155 160 gtc cga gcg ctt ggc tcg gta act gct cag cca aga agt aat cag agt 528 Val Arg Ala Leu Gly Ser Val Thr Ala Gln Pro Arg Ser Asn Gln Ser 165 170 175 gag gtg gcc acc cac tgg acg acc gct ctg ggg acc tat tat gcc gat 576 Glu Val Ala Thr His Trp Thr Thr Ala Leu Gly Thr Tyr Tyr Ala Asp 180 185 190 atc gca gtg ggg cgc tgg gag ccg cag cgc gaa gtg gcc agc tat gga 624 Ile Ala Val Gly Arg Trp Glu Pro Gln Arg Glu Val Ala Ser Tyr Gly 195 200 205 agt gga cta atc atg gcg gaa cgg ctt gat cgt gtt gcc tca acc ttc 672 Ser Gly Leu Ile Met Ala Glu Arg Leu Asp Arg Val Ala Ser Thr Phe 210 215 220 att gca gat ctc tga 687 Ile Ala Asp Leu 225 14 228 PRT Pseudomonas sp. 14 Met Thr Thr Arg Arg Asn Phe Leu Ile Gly Ala Ser Gln Val Gly Ala 1 5 10 15 Leu Val Met Met Ser Pro Lys Leu Val Phe Arg Thr Pro Leu Lys Gln 20 25 30 Lys Pro Val Arg Ile Leu Ser Thr Gly Leu Ala Gly Glu Gln Glu Phe 35 40 45 His Ser Met Leu Arg Ala Arg Leu Thr His Thr Gly Gln Val Asp Ile 50 55 60 Ala Ser Val Pro Leu Asp Ala Ala Ile Trp Ala Ser Pro Ala Arg Leu 65 70 75 80 Ala Gln Ala Met Asp Ala Leu Asn Gly Thr Arg Leu Ile Ala Phe Val 85 90 95 Glu Pro Arg Asn Glu Leu Ile Leu Met Gln Phe Leu Met Asp Arg Gly 100 105 110 Ala Ala Val Leu Ile Gln Gly Glu His Ala Val Asp Ser Lys Gly Val 115 120 125 Ser Arg His Asp Phe Leu Ser Thr Pro Ser Ser Ala Gly Ile Gly Gly 130 135 140 Ala Leu Ala Asp Ser Leu Ala Lys Gly Gly Ser Pro Phe Ser Ile Ser 145 150 155 160 Val Arg Ala Leu Gly Ser Val Thr Ala Gln Pro Arg Ser Asn Gln Ser 165 170 175 Glu Val Ala Thr His Trp Thr Thr Ala Leu Gly Thr Tyr Tyr Ala Asp 180 185 190 Ile Ala Val Gly Arg Trp Glu Pro Gln Arg Glu Val Ala Ser Tyr Gly 195 200 205 Ser Gly Leu Ile Met Ala Glu Arg Leu Asp Arg Val Ala Ser Thr Phe 210 215 220 Ile Ala Asp Leu 225 15 1554 DNA Pseudomonas sp. CDS (1)..(1551) product = “ Flavoprotein UE-Eugenol-Hydroxylase ” /gene = “ehyB” 15 atg gaa agc acc gta gtt ctt ccc gag ggt gtc acc ccg gag cag ttc 48 Met Glu Ser Thr Val Val Leu Pro Glu Gly Val Thr Pro Glu Gln Phe 1 5 10 15 acc aaa gcc atc agc gag ttc cgt cag gta ttg ggt gag gac agt gtt 96 Thr Lys Ala Ile Ser Glu Phe Arg Gln Val Leu Gly Glu Asp Ser Val 20 25 30 ctt gtc act gct gaa cga gtt gtt ccc tat acg aaa ctc ctc att cct 144 Leu Val Thr Ala Glu Arg Val Val Pro Tyr Thr Lys Leu Leu Ile Pro 35 40 45 aca cag gat gat gcc cag tac acc ccg gcc ggt gcc ttg act cct tct 192 Thr Gln Asp Asp Ala Gln Tyr Thr Pro Ala Gly Ala Leu Thr Pro Ser 50 55 60 tcg gtg gag cag gtc cag aaa gtc atg ggg atc tgc aat aag tac aag 240 Ser Val Glu Gln Val Gln Lys Val Met Gly Ile Cys Asn Lys Tyr Lys 65 70 75 80 atc ccg gta tgg cca atc tct acc ggt cgg aac tgg ggg tat ggg tcc 288 Ile Pro Val Trp Pro Ile Ser Thr Gly Arg Asn Trp Gly Tyr Gly Ser 85 90 95 gct tcg cct gca act cct ggg cag atg att ctt gac ctt cgc aag atg 336 Ala Ser Pro Ala Thr Pro Gly Gln Met Ile Leu Asp Leu Arg Lys Met 100 105 110 aac aag atc att gag atc gat gtt gag ggg tgt act gcc ctg ctc gag 384 Asn Lys Ile Ile Glu Ile Asp Val Glu Gly Cys Thr Ala Leu Leu Glu 115 120 125 ccg ggc gtt acc tac cag cag ctt cac gat tac atc aag gag cac aat 432 Pro Gly Val Thr Tyr Gln Gln Leu His Asp Tyr Ile Lys Glu His Asn 130 135 140 ctg ccc ttg atg ctg gat gtg ccg act att ggg cct atg gtt ggc ccg 480 Leu Pro Leu Met Leu Asp Val Pro Thr Ile Gly Pro Met Val Gly Pro 145 150 155 160 gtg ggt aac acg ctg gat cga ggc gtt ggt tat acg ccg tac ggc gag 528 Val Gly Asn Thr Leu Asp Arg Gly Val Gly Tyr Thr Pro Tyr Gly Glu 165 170 175 cac ttc atg atg cag tgt ggt atg gaa gtc gtc atg gcc gat ggc gaa 576 His Phe Met Met Gln Cys Gly Met Glu Val Val Met Ala Asp Gly Glu 180 185 190 atc ctc cgt act ggt atg ggc tcg gtg ccc aaa gcc aag act tgg cag 624 Ile Leu Arg Thr Gly Met Gly Ser Val Pro Lys Ala Lys Thr Trp Gln 195 200 205 gca ttc aaa tgg ggc tat ggt cca tat ctg gac ggt atc ttt acc cag 672 Ala Phe Lys Trp Gly Tyr Gly Pro Tyr Leu Asp Gly Ile Phe Thr Gln 210 215 220 tcc aac ttt ggt gtt gtg aca aag ctc ggg att tgg ttg atg ccc aag 720 Ser Asn Phe Gly Val Val Thr Lys Leu Gly Ile Trp Leu Met Pro Lys 225 230 235 240 ccg cca gtg atc aag tcg ttt atg atc cgt tat ccc aat gaa gct gat 768 Pro Pro Val Ile Lys Ser Phe Met Ile Arg Tyr Pro Asn Glu Ala Asp 245 250 255 gtg gtt aag gca att gat gct ttt cgc ccg ctg cgt att act cag ctg 816 Val Val Lys Ala Ile Asp Ala Phe Arg Pro Leu Arg Ile Thr Gln Leu 260 265 270 att cct aac gtc gtt ttg ttc atg cac ggc atg tac gaa acg gca atc 864 Ile Pro Asn Val Val Leu Phe Met His Gly Met Tyr Glu Thr Ala Ile 275 280 285 tgc cgg acg cgt gct gag gtt act tcg gac cca ggt cct att tct gaa 912 Cys Arg Thr Arg Ala Glu Val Thr Ser Asp Pro Gly Pro Ile Ser Glu 290 295 300 gcg gac gcc cgc aaa gca ttc aaa gag cta ggc gtt ggc tac tgg aac 960 Ala Asp Ala Arg Lys Ala Phe Lys Glu Leu Gly Val Gly Tyr Trp Asn 305 310 315 320 gtt tac ttc gcg ctt tac ggc aca gaa gag cag ata gcc gtc aat gaa 1008 Val Tyr Phe Ala Leu Tyr Gly Thr Glu Glu Gln Ile Ala Val Asn Glu 325 330 335 aag atc gtc cgc ggc atc ctc gaa ccg acg ggg ggt gag atc ctc acc 1056 Lys Ile Val Arg Gly Ile Leu Glu Pro Thr Gly Gly Glu Ile Leu Thr 340 345 350 gaa gag gag gct gga gat aac att ctt ttc cat cac cat aag cag ctc 1104 Glu Glu Glu Ala Gly Asp Asn Ile Leu Phe His His His Lys Gln Leu 355 360 365 atg aac ggc gag atg aca ttg gag gaa atg aat atc tac cag tgg cgc 1152 Met Asn Gly Glu Met Thr Leu Glu Glu Met Asn Ile Tyr Gln Trp Arg 370 375 380 gga gca ggt ggc ggt gct tgc tgg ttt gca ccg gtt gct cag gtc aag 1200 Gly Ala Gly Gly Gly Ala Cys Trp Phe Ala Pro Val Ala Gln Val Lys 385 390 395 400 ggg cat gag gca gag cag cag gtc aag ctt gct cag aag gtg ctt gca 1248 Gly His Glu Ala Glu Gln Gln Val Lys Leu Ala Gln Lys Val Leu Ala 405 410 415 aag cat ggg ttc gat tac acg gcg ggc ttt gcg att ggt tgg cgc gat 1296 Lys His Gly Phe Asp Tyr Thr Ala Gly Phe Ala Ile Gly Trp Arg Asp 420 425 430 ctt cac cat gtg atc gat gtg ctg tac gac cgt agc aat gcc gac gag 1344 Leu His His Val Ile Asp Val Leu Tyr Asp Arg Ser Asn Ala Asp Glu 435 440 445 aaa aag cgc gct tac gct tgc ttt gat gaa ttg atc gac gtc ttt gcg 1392 Lys Lys Arg Ala Tyr Ala Cys Phe Asp Glu Leu Ile Asp Val Phe Ala 450 455 460 gcc gaa ggc ttt gca agt tac agg acc aat att gcc ttt atg gac aaa 1440 Ala Glu Gly Phe Ala Ser Tyr Arg Thr Asn Ile Ala Phe Met Asp Lys 465 470 475 480 gtc gcc tct aag ttc ggc gct gag aat aag agg gtc aat cag aag atc 1488 Val Ala Ser Lys Phe Gly Ala Glu Asn Lys Arg Val Asn Gln Lys Ile 485 490 495 aag gct gcc ctt gat cca aac ggc atc atc gct ccc ggc aag tcg ggc 1536 Lys Ala Ala Leu Asp Pro Asn Gly Ile Ile Ala Pro Gly Lys Ser Gly 500 505 510 att cat ctt ccc aaa taa 1554 Ile His Leu Pro Lys 515 16 517 PRT Pseudomonas sp. 16 Met Glu Ser Thr Val Val Leu Pro Glu Gly Val Thr Pro Glu Gln Phe 1 5 10 15 Thr Lys Ala Ile Ser Glu Phe Arg Gln Val Leu Gly Glu Asp Ser Val 20 25 30 Leu Val Thr Ala Glu Arg Val Val Pro Tyr Thr Lys Leu Leu Ile Pro 35 40 45 Thr Gln Asp Asp Ala Gln Tyr Thr Pro Ala Gly Ala Leu Thr Pro Ser 50 55 60 Ser Val Glu Gln Val Gln Lys Val Met Gly Ile Cys Asn Lys Tyr Lys 65 70 75 80 Ile Pro Val Trp Pro Ile Ser Thr Gly Arg Asn Trp Gly Tyr Gly Ser 85 90 95 Ala Ser Pro Ala Thr Pro Gly Gln Met Ile Leu Asp Leu Arg Lys Met 100 105 110 Asn Lys Ile Ile Glu Ile Asp Val Glu Gly Cys Thr Ala Leu Leu Glu 115 120 125 Pro Gly Val Thr Tyr Gln Gln Leu His Asp Tyr Ile Lys Glu His Asn 130 135 140 Leu Pro Leu Met Leu Asp Val Pro Thr Ile Gly Pro Met Val Gly Pro 145 150 155 160 Val Gly Asn Thr Leu Asp Arg Gly Val Gly Tyr Thr Pro Tyr Gly Glu 165 170 175 His Phe Met Met Gln Cys Gly Met Glu Val Val Met Ala Asp Gly Glu 180 185 190 Ile Leu Arg Thr Gly Met Gly Ser Val Pro Lys Ala Lys Thr Trp Gln 195 200 205 Ala Phe Lys Trp Gly Tyr Gly Pro Tyr Leu Asp Gly Ile Phe Thr Gln 210 215 220 Ser Asn Phe Gly Val Val Thr Lys Leu Gly Ile Trp Leu Met Pro Lys 225 230 235 240 Pro Pro Val Ile Lys Ser Phe Met Ile Arg Tyr Pro Asn Glu Ala Asp 245 250 255 Val Val Lys Ala Ile Asp Ala Phe Arg Pro Leu Arg Ile Thr Gln Leu 260 265 270 Ile Pro Asn Val Val Leu Phe Met His Gly Met Tyr Glu Thr Ala Ile 275 280 285 Cys Arg Thr Arg Ala Glu Val Thr Ser Asp Pro Gly Pro Ile Ser Glu 290 295 300 Ala Asp Ala Arg Lys Ala Phe Lys Glu Leu Gly Val Gly Tyr Trp Asn 305 310 315 320 Val Tyr Phe Ala Leu Tyr Gly Thr Glu Glu Gln Ile Ala Val Asn Glu 325 330 335 Lys Ile Val Arg Gly Ile Leu Glu Pro Thr Gly Gly Glu Ile Leu Thr 340 345 350 Glu Glu Glu Ala Gly Asp Asn Ile Leu Phe His His His Lys Gln Leu 355 360 365 Met Asn Gly Glu Met Thr Leu Glu Glu Met Asn Ile Tyr Gln Trp Arg 370 375 380 Gly Ala Gly Gly Gly Ala Cys Trp Phe Ala Pro Val Ala Gln Val Lys 385 390 395 400 Gly His Glu Ala Glu Gln Gln Val Lys Leu Ala Gln Lys Val Leu Ala 405 410 415 Lys His Gly Phe Asp Tyr Thr Ala Gly Phe Ala Ile Gly Trp Arg Asp 420 425 430 Leu His His Val Ile Asp Val Leu Tyr Asp Arg Ser Asn Ala Asp Glu 435 440 445 Lys Lys Arg Ala Tyr Ala Cys Phe Asp Glu Leu Ile Asp Val Phe Ala 450 455 460 Ala Glu Gly Phe Ala Ser Tyr Arg Thr Asn Ile Ala Phe Met Asp Lys 465 470 475 480 Val Ala Ser Lys Phe Gly Ala Glu Asn Lys Arg Val Asn Gln Lys Ile 485 490 495 Lys Ala Ala Leu Asp Pro Asn Gly Ile Ile Ala Pro Gly Lys Ser Gly 500 505 510 Ile His Leu Pro Lys 515 17 861 DNA Pseudomonas sp. CDS (1)..(858) gene = “ORF2” 17 atg att gca atc act gcg ggc acc gga agt ctt ggt cgg gct atc gtt 48 Met Ile Ala Ile Thr Ala Gly Thr Gly Ser Leu Gly Arg Ala Ile Val 1 5 10 15 gag cga cta ggg gac tgc ggt ctt atc ggt caa gtt cga ttg acg gct 96 Glu Arg Leu Gly Asp Cys Gly Leu Ile Gly Gln Val Arg Leu Thr Ala 20 25 30 cgc gat cct aaa agg ctt cgt gcc gct gcc gag gaa ggg ttt cag gtc 144 Arg Asp Pro Lys Arg Leu Arg Ala Ala Ala Glu Glu Gly Phe Gln Val 35 40 45 gct aag gcg gat tac gcc gat att ggg agt ctt gac cag gca tta cag 192 Ala Lys Ala Asp Tyr Ala Asp Ile Gly Ser Leu Asp Gln Ala Leu Gln 50 55 60 ggg gta gac gta tta ctc ctg att tct ggt act gca ccc aat gaa ata 240 Gly Val Asp Val Leu Leu Leu Ile Ser Gly Thr Ala Pro Asn Glu Ile 65 70 75 80 agg atc caa cag cat aag tcg gtc atc gac gcg gca aaa cga aac ggc 288 Arg Ile Gln Gln His Lys Ser Val Ile Asp Ala Ala Lys Arg Asn Gly 85 90 95 gtg tcg cgt att gtg tat acc agc ttc ata aat cca agt act cgc agc 336 Val Ser Arg Ile Val Tyr Thr Ser Phe Ile Asn Pro Ser Thr Arg Ser 100 105 110 agg tct att tgg gcc tcc att cat cgt gaa act gag act tac ctc agg 384 Arg Ser Ile Trp Ala Ser Ile His Arg Glu Thr Glu Thr Tyr Leu Arg 115 120 125 cag tct ggg gtg aag ttt acg att gtc cga aat aat cag tat gcg tct 432 Gln Ser Gly Val Lys Phe Thr Ile Val Arg Asn Asn Gln Tyr Ala Ser 130 135 140 aac ctg gat ctg ttg ctg ctg agg gct caa gac agc gga ata ttt gcc 480 Asn Leu Asp Leu Leu Leu Leu Arg Ala Gln Asp Ser Gly Ile Phe Ala 145 150 155 160 att ccc ggg gcg aag ggg cgg gtg gcg tac gtc tct cat cgc gac gtt 528 Ile Pro Gly Ala Lys Gly Arg Val Ala Tyr Val Ser His Arg Asp Val 165 170 175 gcc gct gcc atc tgt agt gtc ctg acg acc gcc gga cac gat aac agg 576 Ala Ala Ala Ile Cys Ser Val Leu Thr Thr Ala Gly His Asp Asn Arg 180 185 190 atc tac cag ctc aca ggc tct gag gct ctc aat ggg ctc gag atc gcg 624 Ile Tyr Gln Leu Thr Gly Ser Glu Ala Leu Asn Gly Leu Glu Ile Ala 195 200 205 gag att ctt ggt ggg gtg ctc ggg cgt cca gtg cgc gcg atg gat gcc 672 Glu Ile Leu Gly Gly Val Leu Gly Arg Pro Val Arg Ala Met Asp Ala 210 215 220 tcg cct gac gag ttt gct gcc agc ttt cgc gag gct gga ttc cct gag 720 Ser Pro Asp Glu Phe Ala Ala Ser Phe Arg Glu Ala Gly Phe Pro Glu 225 230 235 240 ttt atg gtt gaa ggc cta cta agc att tat gcc gct tca ggt gct ggg 768 Phe Met Val Glu Gly Leu Leu Ser Ile Tyr Ala Ala Ser Gly Ala Gly 245 250 255 gag tac caa tcc gtc agt cct gat gtt ggg ttg ttg acg gga cga cgt 816 Glu Tyr Gln Ser Val Ser Pro Asp Val Gly Leu Leu Thr Gly Arg Arg 260 265 270 gcc gaa tcg atg cga act tac ata cag cgt cta gtt tgg cct tga 861 Ala Glu Ser Met Arg Thr Tyr Ile Gln Arg Leu Val Trp Pro 275 280 285 18 286 PRT Pseudomonas sp. 18 Met Ile Ala Ile Thr Ala Gly Thr Gly Ser Leu Gly Arg Ala Ile Val 1 5 10 15 Glu Arg Leu Gly Asp Cys Gly Leu Ile Gly Gln Val Arg Leu Thr Ala 20 25 30 Arg Asp Pro Lys Arg Leu Arg Ala Ala Ala Glu Glu Gly Phe Gln Val 35 40 45 Ala Lys Ala Asp Tyr Ala Asp Ile Gly Ser Leu Asp Gln Ala Leu Gln 50 55 60 Gly Val Asp Val Leu Leu Leu Ile Ser Gly Thr Ala Pro Asn Glu Ile 65 70 75 80 Arg Ile Gln Gln His Lys Ser Val Ile Asp Ala Ala Lys Arg Asn Gly 85 90 95 Val Ser Arg Ile Val Tyr Thr Ser Phe Ile Asn Pro Ser Thr Arg Ser 100 105 110 Arg Ser Ile Trp Ala Ser Ile His Arg Glu Thr Glu Thr Tyr Leu Arg 115 120 125 Gln Ser Gly Val Lys Phe Thr Ile Val Arg Asn Asn Gln Tyr Ala Ser 130 135 140 Asn Leu Asp Leu Leu Leu Leu Arg Ala Gln Asp Ser Gly Ile Phe Ala 145 150 155 160 Ile Pro Gly Ala Lys Gly Arg Val Ala Tyr Val Ser His Arg Asp Val 165 170 175 Ala Ala Ala Ile Cys Ser Val Leu Thr Thr Ala Gly His Asp Asn Arg 180 185 190 Ile Tyr Gln Leu Thr Gly Ser Glu Ala Leu Asn Gly Leu Glu Ile Ala 195 200 205 Glu Ile Leu Gly Gly Val Leu Gly Arg Pro Val Arg Ala Met Asp Ala 210 215 220 Ser Pro Asp Glu Phe Ala Ala Ser Phe Arg Glu Ala Gly Phe Pro Glu 225 230 235 240 Phe Met Val Glu Gly Leu Leu Ser Ile Tyr Ala Ala Ser Gly Ala Gly 245 250 255 Glu Tyr Gln Ser Val Ser Pro Asp Val Gly Leu Leu Thr Gly Arg Arg 260 265 270 Ala Glu Ser Met Arg Thr Tyr Ile Gln Arg Leu Val Trp Pro 275 280 285 19 1011 DNA Pseudomonas sp. CDS (1)..(1008) product = “Alkohol-Dehydrogenase” / gene = “adh” 19 atg aag gct tat gag ctt cac aag att tcg gaa cag gta gag gtc agg 48 Met Lys Ala Tyr Glu Leu His Lys Ile Ser Glu Gln Val Glu Val Arg 1 5 10 15 ctc cag cca act cgg ccc cgc ccg cag ttg aat cat ggc gag gtc ctc 96 Leu Gln Pro Thr Arg Pro Arg Pro Gln Leu Asn His Gly Glu Val Leu 20 25 30 atc agg gtc cat gca gcc tcg ctc aac ttt cgc gat ttg atg atc ttg 144 Ile Arg Val His Ala Ala Ser Leu Asn Phe Arg Asp Leu Met Ile Leu 35 40 45 gcc ggt cgc tat ccg ggt caa atg aaa ccc gat gtg atc ccg ctg tcc 192 Ala Gly Arg Tyr Pro Gly Gln Met Lys Pro Asp Val Ile Pro Leu Ser 50 55 60 gat ggt gct ggc gag att gtg gag gtc ggg cct ggc gta tct tcg gag 240 Asp Gly Ala Gly Glu Ile Val Glu Val Gly Pro Gly Val Ser Ser Glu 65 70 75 80 gtg cag ggt cag cgc gta gcc agc acc ttt ttc cct aac tgg cgg gcc 288 Val Gln Gly Gln Arg Val Ala Ser Thr Phe Phe Pro Asn Trp Arg Ala 85 90 95 gga aag att acc gag ccg gct att gag gtg tcg ttg ggc ttc ggt atg 336 Gly Lys Ile Thr Glu Pro Ala Ile Glu Val Ser Leu Gly Phe Gly Met 100 105 110 gac ggg atg ctc gcg gaa tac gtt gct ctg ccc tat gag gca acg ata 384 Asp Gly Met Leu Ala Glu Tyr Val Ala Leu Pro Tyr Glu Ala Thr Ile 115 120 125 ccg ata ccg gag cac ctg tcg tac gag gag gct gca aca ttg cct tgc 432 Pro Ile Pro Glu His Leu Ser Tyr Glu Glu Ala Ala Thr Leu Pro Cys 130 135 140 gcg gcg cta acc gct tgg aat gcg ttg acc gaa gtg ggg cgt gtc aag 480 Ala Ala Leu Thr Ala Trp Asn Ala Leu Thr Glu Val Gly Arg Val Lys 145 150 155 160 gcc ggt gat acg gtc ttg ttg ctt ggc act ggc ggt gtc tcg atg ttc 528 Ala Gly Asp Thr Val Leu Leu Leu Gly Thr Gly Gly Val Ser Met Phe 165 170 175 gcg ttg cag ttc gcc aag ctc ttg ggg gcg acg gtc att cac acc tcg 576 Ala Leu Gln Phe Ala Lys Leu Leu Gly Ala Thr Val Ile His Thr Ser 180 185 190 agc agt gaa caa aag ctg gag agg gtg aaa gcg atg ggg gct gat cat 624 Ser Ser Glu Gln Lys Leu Glu Arg Val Lys Ala Met Gly Ala Asp His 195 200 205 ctg atc aac tac cgc aat tcg cca ggg tgg gac cgt act gtc ctg gat 672 Leu Ile Asn Tyr Arg Asn Ser Pro Gly Trp Asp Arg Thr Val Leu Asp 210 215 220 ctc acc gcg ggg cga ggg gtt gac ctg gta gtc gag gta ggg ggg gcg 720 Leu Thr Ala Gly Arg Gly Val Asp Leu Val Val Glu Val Gly Gly Ala 225 230 235 240 ggg acc ttg gag cgc tca ctt cgt gcg gtc aag gta ggc ggt att gtc 768 Gly Thr Leu Glu Arg Ser Leu Arg Ala Val Lys Val Gly Gly Ile Val 245 250 255 gcc acg att ggg cta gtg gct ggc gtt ggc ccg att gac cca ttg ccg 816 Ala Thr Ile Gly Leu Val Ala Gly Val Gly Pro Ile Asp Pro Leu Pro 260 265 270 ctt atc tcc agg gct att cag ctc tcg ggc gtc tat gtc ggt tcc cgg 864 Leu Ile Ser Arg Ala Ile Gln Leu Ser Gly Val Tyr Val Gly Ser Arg 275 280 285 gaa atg ttt ctc tca atg aac aaa gcc att gca tca gcc gaa atc aag 912 Glu Met Phe Leu Ser Met Asn Lys Ala Ile Ala Ser Ala Glu Ile Lys 290 295 300 cca gtg atc gat tgc tgc ttc ccc atc gac gag gtt gga gat gct tat 960 Pro Val Ile Asp Cys Cys Phe Pro Ile Asp Glu Val Gly Asp Ala Tyr 305 310 315 320 gag tac atg cgt agc ggc aat cac ctt ggc aaa gta gtt atc acg atc 1008 Glu Tyr Met Arg Ser Gly Asn His Leu Gly Lys Val Val Ile Thr Ile 325 330 335 taa 1011 20 336 PRT Pseudomonas sp. 20 Met Lys Ala Tyr Glu Leu His Lys Ile Ser Glu Gln Val Glu Val Arg 1 5 10 15 Leu Gln Pro Thr Arg Pro Arg Pro Gln Leu Asn His Gly Glu Val Leu 20 25 30 Ile Arg Val His Ala Ala Ser Leu Asn Phe Arg Asp Leu Met Ile Leu 35 40 45 Ala Gly Arg Tyr Pro Gly Gln Met Lys Pro Asp Val Ile Pro Leu Ser 50 55 60 Asp Gly Ala Gly Glu Ile Val Glu Val Gly Pro Gly Val Ser Ser Glu 65 70 75 80 Val Gln Gly Gln Arg Val Ala Ser Thr Phe Phe Pro Asn Trp Arg Ala 85 90 95 Gly Lys Ile Thr Glu Pro Ala Ile Glu Val Ser Leu Gly Phe Gly Met 100 105 110 Asp Gly Met Leu Ala Glu Tyr Val Ala Leu Pro Tyr Glu Ala Thr Ile 115 120 125 Pro Ile Pro Glu His Leu Ser Tyr Glu Glu Ala Ala Thr Leu Pro Cys 130 135 140 Ala Ala Leu Thr Ala Trp Asn Ala Leu Thr Glu Val Gly Arg Val Lys 145 150 155 160 Ala Gly Asp Thr Val Leu Leu Leu Gly Thr Gly Gly Val Ser Met Phe 165 170 175 Ala Leu Gln Phe Ala Lys Leu Leu Gly Ala Thr Val Ile His Thr Ser 180 185 190 Ser Ser Glu Gln Lys Leu Glu Arg Val Lys Ala Met Gly Ala Asp His 195 200 205 Leu Ile Asn Tyr Arg Asn Ser Pro Gly Trp Asp Arg Thr Val Leu Asp 210 215 220 Leu Thr Ala Gly Arg Gly Val Asp Leu Val Val Glu Val Gly Gly Ala 225 230 235 240 Gly Thr Leu Glu Arg Ser Leu Arg Ala Val Lys Val Gly Gly Ile Val 245 250 255 Ala Thr Ile Gly Leu Val Ala Gly Val Gly Pro Ile Asp Pro Leu Pro 260 265 270 Leu Ile Ser Arg Ala Ile Gln Leu Ser Gly Val Tyr Val Gly Ser Arg 275 280 285 Glu Met Phe Leu Ser Met Asn Lys Ala Ile Ala Ser Ala Glu Ile Lys 290 295 300 Pro Val Ile Asp Cys Cys Phe Pro Ile Asp Glu Val Gly Asp Ala Tyr 305 310 315 320 Glu Tyr Met Arg Ser Gly Asn His Leu Gly Lys Val Val Ile Thr Ile 325 330 335 21 1518 DNA Pseudomonas sp. 21 tcaccgtcgt gatcgggatt ggaaattcgt gcgaggacag cggccacgta ccggcgccct 60 gaagggctgg aaggttggag tttcgttaag gtctggtacc cagcagccat ggagagcggc 120 ccttagccgg aatggcagct tgatggttgc cacgggacca gactggatgt cttgagtgtc 180 gagaattacc agatcgctgc gattttcatc gaggcgacca accacggtca gcaagtaccc 240 gtcaccttcg gcggcggtcg gacttctagg gacgaaggcc ggctcctggg ccgccgaggc 300 ttcgccggag taccagaggt cgtagtcacc tcggtggttg tcccagatgc cgagtgagtt 360 gtacgcgaat atcttctcgg cctgctgatg cgcaagtggt ttgcgtggat cgtccacccc 420 cataaagcca tagcggttgc attgcagggc gaacgaagaa tccatgattg gcatttccgc 480 aaagaaatcg tgtagccggg ttcgcttgat ctcgtcgctg ctgctatcga ggtcaatttc 540 ccaacgagtc aggcgtggta cggctttctc aggggcgaag ggttggtttt gtgagttggg 600 gaaggggaac ggcaggattt cactttccat aaggtcgata taaatcttgg ttccgacttc 660 ccaagcattc acaacatgaa atacccagag cgccggtgcc ttgagccagc gaatcagact 720 gccctggcgc ggcgcgagta cgccaatgta gctgcccagt tccggctccc acatataaat 780 tggctgtttc gccttgaggc gggacaggct gttggtggcc ggcataattg ggaaaatgga 840 ccaatttcgg gtaatggcaa agtcgtgcat gaatgcgcca tagggctgct caaaccaagt 900 ttcatgtgtc accttgccgt gcttgtcgac aatgtaatag gccatgtctg gagttgcttc 960 gcccttagct gccgaaccga agaacaacaa gtcacccgtt tccgggtcat attttggatg 1020 ggcggtgtgg gtttggctgg taacttggcc gtcgtagtcg aagtgtccgc gagtttcaag 1080 tgtacgagga tccagttcgt acggtaggcc gtcttccttc accgccagca ccttgccgtg 1140 atggctaatg atgcttgtat tggcaacggt gcggtctagt ccttttacac tggtgtcgtc 1200 ggtatagggg tttctgtaca tgccaaatag cgattttcgc gctagtcgtt cggccgtgaa 1260 tcgagcggtt ttaacccagc gactgatgaa gtcgacatga ccatcttcga agtggaaggc 1320 agaggccatt ccatctccat ctatgaaggt gtggaatttt tgtggggtaa cttgaggctc 1380 tggcgtatta cggtagaacg ttccatttat tgattttggg atttcgccgt caacctctag 1440 atcgaacaag tctgcctcta tacgggtggg gagaagtgtt cctactaatt gcgggtcgtt 1500 gcggttgaat ctcgccat 1518 22 505 PRT Pseudomonas sp. 22 Met Ala Arg Phe Asn Arg Asn Asp Pro Gln Leu Val Gly Thr Leu Leu 1 5 10 15 Pro Thr Arg Ile Glu Ala Asp Leu Phe Asp Leu Glu Val Asp Gly Glu 20 25 30 Ile Pro Lys Ser Ile Asn Gly Thr Phe Tyr Arg Asn Thr Pro Glu Pro 35 40 45 Gln Val Thr Pro Gln Lys Phe His Thr Phe Ile Asp Gly Asp Gly Met 50 55 60 Ala Ser Ala Phe His Phe Glu Asp Gly His Val Asp Phe Ile Ser Arg 65 70 75 80 Trp Val Lys Thr Ala Arg Phe Thr Ala Glu Arg Leu Ala Arg Lys Ser 85 90 95 Leu Phe Gly Met Tyr Arg Asn Pro Tyr Thr Asp Asp Thr Ser Val Lys 100 105 110 Gly Leu Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Ser His His Gly 115 120 125 Lys Val Leu Ala Val Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro 130 135 140 Arg Thr Leu Glu Thr Arg Gly His Phe Asp Tyr Asp Gly Gln Val Thr 145 150 155 160 Ser Gln Thr His Thr Ala His Pro Lys Tyr Asp Pro Glu Thr Gly Asp 165 170 175 Leu Leu Phe Phe Gly Ser Ala Ala Lys Gly Glu Ala Thr Pro Asp Met 180 185 190 Ala Tyr Tyr Ile Val Asp Lys His Gly Lys Val Thr His Glu Thr Trp 195 200 205 Phe Glu Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Ile Thr Arg 210 215 220 Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Ser Arg 225 230 235 240 Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Leu Gly Ser 245 250 255 Tyr Ile Gly Val Leu Ala Pro Arg Gln Gly Ser Leu Ile Arg Trp Leu 260 265 270 Lys Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu Val 275 280 285 Gly Thr Lys Ile Tyr Ile Asp Leu Met Glu Ser Glu Ile Leu Pro Phe 290 295 300 Pro Phe Pro Asn Ser Gln Asn Gln Pro Phe Ala Pro Glu Lys Ala Val 305 310 315 320 Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asp Ser Ser Ser Asp Glu 325 330 335 Ile Lys Arg Thr Arg Leu His Asp Phe Phe Ala Glu Met Pro Ile Met 340 345 350 Asp Ser Ser Phe Ala Leu Gln Cys Asn Arg Tyr Gly Phe Met Gly Val 355 360 365 Asp Asp Pro Arg Lys Pro Leu Ala His Gln Gln Ala Glu Lys Ile Phe 370 375 380 Ala Tyr Asn Ser Leu Gly Ile Trp Asp Asn His Arg Gly Asp Tyr Asp 385 390 395 400 Leu Trp Tyr Ser Gly Glu Ala Ser Ala Ala Gln Glu Pro Ala Phe Val 405 410 415 Pro Arg Ser Pro Thr Ala Ala Glu Gly Asp Gly Tyr Leu Leu Thr Val 420 425 430 Val Gly Arg Leu Asp Glu Asn Arg Ser Asp Leu Val Ile Leu Asp Thr 435 440 445 Gln Asp Ile Gln Ser Gly Pro Val Ala Thr Ile Lys Leu Pro Phe Arg 450 455 460 Leu Arg Ala Ala Leu His Gly Cys Trp Val Pro Asp Leu Asn Glu Thr 465 470 475 480 Pro Thr Phe Gln Pro Phe Arg Ala Pro Val Arg Gly Arg Cys Pro Arg 485 490 495 Thr Asn Phe Gln Ser Arg Ser Arg Arg 500 505 23 951 DNA Pseudomonas sp. CDS (1)..(948) gene = “ORF3” 23 atg aca act att cgg tgg cgg cgt atg tcc att cac tct gag ggg atc 48 Met Thr Thr Ile Arg Trp Arg Arg Met Ser Ile His Ser Glu Gly Ile 1 5 10 15 act ctc gcg gat tcg ccg ctg cat tgg gcg cat acc ctg aat gga tca 96 Thr Leu Ala Asp Ser Pro Leu His Trp Ala His Thr Leu Asn Gly Ser 20 25 30 atg cgt act cat ttc gaa gtc cag cgt ctt gag cgg ggt aga ggt gcc 144 Met Arg Thr His Phe Glu Val Gln Arg Leu Glu Arg Gly Arg Gly Ala 35 40 45 tcc ctt gcc cga tct aga ttt ggc gcg ggt gag ctg tac agt gcc att 192 Ser Leu Ala Arg Ser Arg Phe Gly Ala Gly Glu Leu Tyr Ser Ala Ile 50 55 60 gca cca agc cag gta ctt cgc cac ttc aac gac cag cga aat gct gat 240 Ala Pro Ser Gln Val Leu Arg His Phe Asn Asp Gln Arg Asn Ala Asp 65 70 75 80 gag gct gag cac agc tat ttg att cag ata cga agt ggc gct ttg ggc 288 Glu Ala Glu His Ser Tyr Leu Ile Gln Ile Arg Ser Gly Ala Leu Gly 85 90 95 gtt gca tcc ggc gga aga aag gtg atc ttg gca aat ggt gat tgc tcc 336 Val Ala Ser Gly Gly Arg Lys Val Ile Leu Ala Asn Gly Asp Cys Ser 100 105 110 ata gtt gat agt cgc caa gac ttc aca ctt tcc tcg aac tct tcg acc 384 Ile Val Asp Ser Arg Gln Asp Phe Thr Leu Ser Ser Asn Ser Ser Thr 115 120 125 caa ggt gtc gta ata cgc ttt ccg gtg agt tgg ctg gga gcg tgg gtg 432 Gln Gly Val Val Ile Arg Phe Pro Val Ser Trp Leu Gly Ala Trp Val 130 135 140 tcc aat ccg gag gat ctt atc gcc cga cga gtt gat gct gag gta ggg 480 Ser Asn Pro Glu Asp Leu Ile Ala Arg Arg Val Asp Ala Glu Val Gly 145 150 155 160 tgg ggt agg gcg cta agc gca tcg gtt tct aat cta gat cca ttg cgc 528 Trp Gly Arg Ala Leu Ser Ala Ser Val Ser Asn Leu Asp Pro Leu Arg 165 170 175 atc gac gat tta ggt agc aat gta aat ggc att gca gag cat gtt gct 576 Ile Asp Asp Leu Gly Ser Asn Val Asn Gly Ile Ala Glu His Val Ala 180 185 190 atg tta att tca cta gca agt tct gcg gtt agt tct gaa gat ggg ggt 624 Met Leu Ile Ser Leu Ala Ser Ser Ala Val Ser Ser Glu Asp Gly Gly 195 200 205 gtg gct ctt cgg aaa atg agg gaa gtg aag aga gta ctc gag cag agt 672 Val Ala Leu Arg Lys Met Arg Glu Val Lys Arg Val Leu Glu Gln Ser 210 215 220 ttc gca gac gct aat ctc ggg ccg gaa agt gtt tca agt caa tta gga 720 Phe Ala Asp Ala Asn Leu Gly Pro Glu Ser Val Ser Ser Gln Leu Gly 225 230 235 240 att tcg aaa cgc tat ttg cat tat gtc ttt gct gcg tgc ggt acg acc 768 Ile Ser Lys Arg Tyr Leu His Tyr Val Phe Ala Ala Cys Gly Thr Thr 245 250 255 ttt ggt cgc gag ctg ttg gaa ata cgc ctg ggc aaa gct tat cga atg 816 Phe Gly Arg Glu Leu Leu Glu Ile Arg Leu Gly Lys Ala Tyr Arg Met 260 265 270 ctc tgt gcg gcg agt gac tcg ggt gct gtg ctg aag gtg gcc atg tcc 864 Leu Cys Ala Ala Ser Asp Ser Gly Ala Val Leu Lys Val Ala Met Ser 275 280 285 tca ggt ttt tcg gat tca agc cat ttc agc aag aaa ttt aag gaa aga 912 Ser Gly Phe Ser Asp Ser Ser His Phe Ser Lys Lys Phe Lys Glu Arg 290 295 300 tac ggt gtt tcg cct gtc tcc ttg gtg agg cag gct tga 951 Tyr Gly Val Ser Pro Val Ser Leu Val Arg Gln Ala 305 310 315 24 316 PRT Pseudomonas sp. 24 Met Thr Thr Ile Arg Trp Arg Arg Met Ser Ile His Ser Glu Gly Ile 1 5 10 15 Thr Leu Ala Asp Ser Pro Leu His Trp Ala His Thr Leu Asn Gly Ser 20 25 30 Met Arg Thr His Phe Glu Val Gln Arg Leu Glu Arg Gly Arg Gly Ala 35 40 45 Ser Leu Ala Arg Ser Arg Phe Gly Ala Gly Glu Leu Tyr Ser Ala Ile 50 55 60 Ala Pro Ser Gln Val Leu Arg His Phe Asn Asp Gln Arg Asn Ala Asp 65 70 75 80 Glu Ala Glu His Ser Tyr Leu Ile Gln Ile Arg Ser Gly Ala Leu Gly 85 90 95 Val Ala Ser Gly Gly Arg Lys Val Ile Leu Ala Asn Gly Asp Cys Ser 100 105 110 Ile Val Asp Ser Arg Gln Asp Phe Thr Leu Ser Ser Asn Ser Ser Thr 115 120 125 Gln Gly Val Val Ile Arg Phe Pro Val Ser Trp Leu Gly Ala Trp Val 130 135 140 Ser Asn Pro Glu Asp Leu Ile Ala Arg Arg Val Asp Ala Glu Val Gly 145 150 155 160 Trp Gly Arg Ala Leu Ser Ala Ser Val Ser Asn Leu Asp Pro Leu Arg 165 170 175 Ile Asp Asp Leu Gly Ser Asn Val Asn Gly Ile Ala Glu His Val Ala 180 185 190 Met Leu Ile Ser Leu Ala Ser Ser Ala Val Ser Ser Glu Asp Gly Gly 195 200 205 Val Ala Leu Arg Lys Met Arg Glu Val Lys Arg Val Leu Glu Gln Ser 210 215 220 Phe Ala Asp Ala Asn Leu Gly Pro Glu Ser Val Ser Ser Gln Leu Gly 225 230 235 240 Ile Ser Lys Arg Tyr Leu His Tyr Val Phe Ala Ala Cys Gly Thr Thr 245 250 255 Phe Gly Arg Glu Leu Leu Glu Ile Arg Leu Gly Lys Ala Tyr Arg Met 260 265 270 Leu Cys Ala Ala Ser Asp Ser Gly Ala Val Leu Lys Val Ala Met Ser 275 280 285 Ser Gly Phe Ser Asp Ser Ser His Phe Ser Lys Lys Phe Lys Glu Arg 290 295 300 Tyr Gly Val Ser Pro Val Ser Leu Val Arg Gln Ala 305 310 315 25 735 DNA Pseudomonas sp. CDS (1)..(732) product = “Enoyl-CoA-Hydratase” / gene = “ech” 25 atg agc cca act ctc aat cga gag atg gtc gag gtt ctg gag gtg ctg 48 Met Ser Pro Thr Leu Asn Arg Glu Met Val Glu Val Leu Glu Val Leu 1 5 10 15 gag cag gac gca gat gct cgc gtg ctt gtt ctg act ggt gca ggc gaa 96 Glu Gln Asp Ala Asp Ala Arg Val Leu Val Leu Thr Gly Ala Gly Glu 20 25 30 tcc tgg acc gcg ggc atg gac ctg aag gag tat ttc cgc gag acc gat 144 Ser Trp Thr Ala Gly Met Asp Leu Lys Glu Tyr Phe Arg Glu Thr Asp 35 40 45 gct ggc ccc gaa att ctg caa gag aag att cgt cgc gaa gcg tcg acc 192 Ala Gly Pro Glu Ile Leu Gln Glu Lys Ile Arg Arg Glu Ala Ser Thr 50 55 60 tgg cag tgg aag ctc ctg cgg atg tac acc aag ccg acc atc gcg atg 240 Trp Gln Trp Lys Leu Leu Arg Met Tyr Thr Lys Pro Thr Ile Ala Met 65 70 75 80 gtc aat ggc tgg tgc ttc ggc ggc ggc ttc agc ccg ctg gtg gcc tgt 288 Val Asn Gly Trp Cys Phe Gly Gly Gly Phe Ser Pro Leu Val Ala Cys 85 90 95 gat ctg gcc atc tgt gcc gac gag gcc acc ttt ggc ctg tcc gag atc 336 Asp Leu Ala Ile Cys Ala Asp Glu Ala Thr Phe Gly Leu Ser Glu Ile 100 105 110 aac tgg ggc atc ccg ccg ggc aac ctg gtg agt aag gct atg gcc gac 384 Asn Trp Gly Ile Pro Pro Gly Asn Leu Val Ser Lys Ala Met Ala Asp 115 120 125 acc gtg ggt cac cgc gag tcc ctt tac tac atc atg act ggc aag aca 432 Thr Val Gly His Arg Glu Ser Leu Tyr Tyr Ile Met Thr Gly Lys Thr 130 135 140 ttt ggc ggt cag cag gcc gcc aag atg ggg ctt gtg aac cag agt gtt 480 Phe Gly Gly Gln Gln Ala Ala Lys Met Gly Leu Val Asn Gln Ser Val 145 150 155 160 ccg ctg gcc gag ctg cgc agt gtc act gta gag ctg gct cag aac ctg 528 Pro Leu Ala Glu Leu Arg Ser Val Thr Val Glu Leu Ala Gln Asn Leu 165 170 175 ctg gac aag aac ccc gta gtg ctg cgt gcc gcc aaa ata ggc ttc aag 576 Leu Asp Lys Asn Pro Val Val Leu Arg Ala Ala Lys Ile Gly Phe Lys 180 185 190 cgt tgc cgc gag ctg act tgg gag cag aac gag gac tac ctg tac gcc 624 Arg Cys Arg Glu Leu Thr Trp Glu Gln Asn Glu Asp Tyr Leu Tyr Ala 195 200 205 aag ctc gac caa tcc cgt ttg ctc gat ccg gaa ggc ggt cgc gag cag 672 Lys Leu Asp Gln Ser Arg Leu Leu Asp Pro Glu Gly Gly Arg Glu Gln 210 215 220 ggc atg aag cag ttc ctt gac gag aaa agc atc aag ccg ggc ttg cag 720 Gly Met Lys Gln Phe Leu Asp Glu Lys Ser Ile Lys Pro Gly Leu Gln 225 230 235 240 acc tac aag cgc tga 735 Thr Tyr Lys Arg 26 244 PRT Pseudomonas sp. 26 Met Ser Pro Thr Leu Asn Arg Glu Met Val Glu Val Leu Glu Val Leu 1 5 10 15 Glu Gln Asp Ala Asp Ala Arg Val Leu Val Leu Thr Gly Ala Gly Glu 20 25 30 Ser Trp Thr Ala Gly Met Asp Leu Lys Glu Tyr Phe Arg Glu Thr Asp 35 40 45 Ala Gly Pro Glu Ile Leu Gln Glu Lys Ile Arg Arg Glu Ala Ser Thr 50 55 60 Trp Gln Trp Lys Leu Leu Arg Met Tyr Thr Lys Pro Thr Ile Ala Met 65 70 75 80 Val Asn Gly Trp Cys Phe Gly Gly Gly Phe Ser Pro Leu Val Ala Cys 85 90 95 Asp Leu Ala Ile Cys Ala Asp Glu Ala Thr Phe Gly Leu Ser Glu Ile 100 105 110 Asn Trp Gly Ile Pro Pro Gly Asn Leu Val Ser Lys Ala Met Ala Asp 115 120 125 Thr Val Gly His Arg Glu Ser Leu Tyr Tyr Ile Met Thr Gly Lys Thr 130 135 140 Phe Gly Gly Gln Gln Ala Ala Lys Met Gly Leu Val Asn Gln Ser Val 145 150 155 160 Pro Leu Ala Glu Leu Arg Ser Val Thr Val Glu Leu Ala Gln Asn Leu 165 170 175 Leu Asp Lys Asn Pro Val Val Leu Arg Ala Ala Lys Ile Gly Phe Lys 180 185 190 Arg Cys Arg Glu Leu Thr Trp Glu Gln Asn Glu Asp Tyr Leu Tyr Ala 195 200 205 Lys Leu Asp Gln Ser Arg Leu Leu Asp Pro Glu Gly Gly Arg Glu Gln 210 215 220 Gly Met Lys Gln Phe Leu Asp Glu Lys Ser Ile Lys Pro Gly Leu Gln 225 230 235 240 Thr Tyr Lys Arg 27 1446 DNA Pseudomonas sp. CDS (1)..(1443) product = Vanillin-Dehydrogenase“ / gene = ”vdh“ 27 atg ttt cac gtg ccc ctg ctt att ggt ggt aag cct tgt tca gca tct 48 Met Phe His Val Pro Leu Leu Ile Gly Gly Lys Pro Cys Ser Ala Ser 1 5 10 15 gat gag cgc acc ttc gag cgt cgt agc ccg ctg acc gga gaa gtg gta 96 Asp Glu Arg Thr Phe Glu Arg Arg Ser Pro Leu Thr Gly Glu Val Val 20 25 30 tcg cgc gtc gct gct gcc agt ttg gaa gat gcg gac gcc gca gtg gcc 144 Ser Arg Val Ala Ala Ala Ser Leu Glu Asp Ala Asp Ala Ala Val Ala 35 40 45 gct gca cag gct gcg ttt cct gaa tgg gcg gcg ctt gct ccg agc gaa 192 Ala Ala Gln Ala Ala Phe Pro Glu Trp Ala Ala Leu Ala Pro Ser Glu 50 55 60 cgc cgt gcc cga ctg ctg cga gcg gcg gat ctt cta gag gac cgt tct 240 Arg Arg Ala Arg Leu Leu Arg Ala Ala Asp Leu Leu Glu Asp Arg Ser 65 70 75 80 tcc gag ttc acc gcc gca gcg agt gaa act ggc gca gcg gga aac tgg 288 Ser Glu Phe Thr Ala Ala Ala Ser Glu Thr Gly Ala Ala Gly Asn Trp 85 90 95 tat ggg ttt aac gtt tac ctg gcg gcg ggc atg ttg cgg gaa gcc gcg 336 Tyr Gly Phe Asn Val Tyr Leu Ala Ala Gly Met Leu Arg Glu Ala Ala 100 105 110 gcc atg acc aca cag att cag ggc gat gtc att ccg tcc aat gtg ccc 384 Ala Met Thr Thr Gln Ile Gln Gly Asp Val Ile Pro Ser Asn Val Pro 115 120 125 ggt agc ttt gcc atg gcg gtt cga cag cca tgt ggc gtg gtg ctc ggt 432 Gly Ser Phe Ala Met Ala Val Arg Gln Pro Cys Gly Val Val Leu Gly 130 135 140 att gcg cct tgg aat gct ccg gta atc ctt ggc gta cgg gct gtt gcg 480 Ile Ala Pro Trp Asn Ala Pro Val Ile Leu Gly Val Arg Ala Val Ala 145 150 155 160 atg ccg ttg gca tgc ggc aat acc gtg gtg ttg aaa agc tct gag ctg 528 Met Pro Leu Ala Cys Gly Asn Thr Val Val Leu Lys Ser Ser Glu Leu 165 170 175 agt ccc ttt acc cat cgc ctg att ggt cag gtg ttg cat gat gct ggt 576 Ser Pro Phe Thr His Arg Leu Ile Gly Gln Val Leu His Asp Ala Gly 180 185 190 ctg ggg gat ggc gtg gtg aat gtc atc agc aat gcc ccg caa gac gct 624 Leu Gly Asp Gly Val Val Asn Val Ile Ser Asn Ala Pro Gln Asp Ala 195 200 205 cct gcg gtg gtg gag cga ctg att gca aat cct gcg gta cgt cga gtg 672 Pro Ala Val Val Glu Arg Leu Ile Ala Asn Pro Ala Val Arg Arg Val 210 215 220 aac ttc acc ggt tcg acc cac gtt gga cgg atc att ggt gag ctg tct 720 Asn Phe Thr Gly Ser Thr His Val Gly Arg Ile Ile Gly Glu Leu Ser 225 230 235 240 gcg cgt cat ctg aag cct gct gtg ctg gaa tta ggt ggt aag gct ccg 768 Ala Arg His Leu Lys Pro Ala Val Leu Glu Leu Gly Gly Lys Ala Pro 245 250 255 ttc ttg gtc ttg gac gat gcc gac ctc gat gcg gcg gtc gaa gcg gcg 816 Phe Leu Val Leu Asp Asp Ala Asp Leu Asp Ala Ala Val Glu Ala Ala 260 265 270 gcc ttt ggt gcc tac ttc aat cag ggt caa atc tgc atg tcc act gag 864 Ala Phe Gly Ala Tyr Phe Asn Gln Gly Gln Ile Cys Met Ser Thr Glu 275 280 285 cgt ctg att gtg aca gca gtc gca gac gcc ttt gtt gaa aag ctg gcg 912 Arg Leu Ile Val Thr Ala Val Ala Asp Ala Phe Val Glu Lys Leu Ala 290 295 300 agg aag gtc gcc aca ctg cgt gct ggc gat cct aat gat ccg caa tcg 960 Arg Lys Val Ala Thr Leu Arg Ala Gly Asp Pro Asn Asp Pro Gln Ser 305 310 315 320 gtc ttg ggt tcg ttg att gat gcc aat gca ggt caa cgc atc cag gtt 1008 Val Leu Gly Ser Leu Ile Asp Ala Asn Ala Gly Gln Arg Ile Gln Val 325 330 335 ctg gtc gat gat gcg ctc gca aaa ggc gcg cgg cag gtc gtc ggt ggt 1056 Leu Val Asp Asp Ala Leu Ala Lys Gly Ala Arg Gln Val Val Gly Gly 340 345 350 ggc tta gat ggc agc atc atg cag ccg atg ctg ctt gat cag gtc act 1104 Gly Leu Asp Gly Ser Ile Met Gln Pro Met Leu Leu Asp Gln Val Thr 355 360 365 gaa gag atg cgg ctc tac cgt gag gag tcc ttt ggc cct gtt gcc gtt 1152 Glu Glu Met Arg Leu Tyr Arg Glu Glu Ser Phe Gly Pro Val Ala Val 370 375 380 gtc ttg cgc ggc gat ggt gat gaa gaa ctg ctg cgt ctt gcc aac gat 1200 Val Leu Arg Gly Asp Gly Asp Glu Glu Leu Leu Arg Leu Ala Asn Asp 385 390 395 400 tcg gag ttt ggt ctt tcg gcc gcc att ttc agc cgt gac gtc tcg cgc 1248 Ser Glu Phe Gly Leu Ser Ala Ala Ile Phe Ser Arg Asp Val Ser Arg 405 410 415 gca atg gaa ttg gcc cag cgc gtc gat tcg ggc att tgc cat atc aat 1296 Ala Met Glu Leu Ala Gln Arg Val Asp Ser Gly Ile Cys His Ile Asn 420 425 430 gga ccg act gtg cat gac gag gct cag atg cca ttc ggt ggg gtg aag 1344 Gly Pro Thr Val His Asp Glu Ala Gln Met Pro Phe Gly Gly Val Lys 435 440 445 tcc agc ggc tac ggc agc ttc ggc agt cga gca tcg att gag cac ttt 1392 Ser Ser Gly Tyr Gly Ser Phe Gly Ser Arg Ala Ser Ile Glu His Phe 450 455 460 acc cag ctg cgc tgg ctg acc att cag aat ggc ccg cgg cac tat cca 1440 Thr Gln Leu Arg Trp Leu Thr Ile Gln Asn Gly Pro Arg His Tyr Pro 465 470 475 480 atc taa 1446 Ile 28 481 PRT Pseudomonas sp. 28 Met Phe His Val Pro Leu Leu Ile Gly Gly Lys Pro Cys Ser Ala Ser 1 5 10 15 Asp Glu Arg Thr Phe Glu Arg Arg Ser Pro Leu Thr Gly Glu Val Val 20 25 30 Ser Arg Val Ala Ala Ala Ser Leu Glu Asp Ala Asp Ala Ala Val Ala 35 40 45 Ala Ala Gln Ala Ala Phe Pro Glu Trp Ala Ala Leu Ala Pro Ser Glu 50 55 60 Arg Arg Ala Arg Leu Leu Arg Ala Ala Asp Leu Leu Glu Asp Arg Ser 65 70 75 80 Ser Glu Phe Thr Ala Ala Ala Ser Glu Thr Gly Ala Ala Gly Asn Trp 85 90 95 Tyr Gly Phe Asn Val Tyr Leu Ala Ala Gly Met Leu Arg Glu Ala Ala 100 105 110 Ala Met Thr Thr Gln Ile Gln Gly Asp Val Ile Pro Ser Asn Val Pro 115 120 125 Gly Ser Phe Ala Met Ala Val Arg Gln Pro Cys Gly Val Val Leu Gly 130 135 140 Ile Ala Pro Trp Asn Ala Pro Val Ile Leu Gly Val Arg Ala Val Ala 145 150 155 160 Met Pro Leu Ala Cys Gly Asn Thr Val Val Leu Lys Ser Ser Glu Leu 165 170 175 Ser Pro Phe Thr His Arg Leu Ile Gly Gln Val Leu His Asp Ala Gly 180 185 190 Leu Gly Asp Gly Val Val Asn Val Ile Ser Asn Ala Pro Gln Asp Ala 195 200 205 Pro Ala Val Val Glu Arg Leu Ile Ala Asn Pro Ala Val Arg Arg Val 210 215 220 Asn Phe Thr Gly Ser Thr His Val Gly Arg Ile Ile Gly Glu Leu Ser 225 230 235 240 Ala Arg His Leu Lys Pro Ala Val Leu Glu Leu Gly Gly Lys Ala Pro 245 250 255 Phe Leu Val Leu Asp Asp Ala Asp Leu Asp Ala Ala Val Glu Ala Ala 260 265 270 Ala Phe Gly Ala Tyr Phe Asn Gln Gly Gln Ile Cys Met Ser Thr Glu 275 280 285 Arg Leu Ile Val Thr Ala Val Ala Asp Ala Phe Val Glu Lys Leu Ala 290 295 300 Arg Lys Val Ala Thr Leu Arg Ala Gly Asp Pro Asn Asp Pro Gln Ser 305 310 315 320 Val Leu Gly Ser Leu Ile Asp Ala Asn Ala Gly Gln Arg Ile Gln Val 325 330 335 Leu Val Asp Asp Ala Leu Ala Lys Gly Ala Arg Gln Val Val Gly Gly 340 345 350 Gly Leu Asp Gly Ser Ile Met Gln Pro Met Leu Leu Asp Gln Val Thr 355 360 365 Glu Glu Met Arg Leu Tyr Arg Glu Glu Ser Phe Gly Pro Val Ala Val 370 375 380 Val Leu Arg Gly Asp Gly Asp Glu Glu Leu Leu Arg Leu Ala Asn Asp 385 390 395 400 Ser Glu Phe Gly Leu Ser Ala Ala Ile Phe Ser Arg Asp Val Ser Arg 405 410 415 Ala Met Glu Leu Ala Gln Arg Val Asp Ser Gly Ile Cys His Ile Asn 420 425 430 Gly Pro Thr Val His Asp Glu Ala Gln Met Pro Phe Gly Gly Val Lys 435 440 445 Ser Ser Gly Tyr Gly Ser Phe Gly Ser Arg Ala Ser Ile Glu His Phe 450 455 460 Thr Gln Leu Arg Trp Leu Thr Ile Gln Asn Gly Pro Arg His Tyr Pro 465 470 475 480 Ile 29 1770 DNA Pseudomonas sp. CDS (1)..(1767) product = ”Ferulasaeure-CoA-Synthetase“ / gene =”fcs“ 29 atg cgt tct ctc gag gcg ctt ctt ccc ttc ccg ggt cga att ctt gag 48 Met Arg Ser Leu Glu Ala Leu Leu Pro Phe Pro Gly Arg Ile Leu Glu 1 5 10 15 cgt ctc gag cat tgg gct aag acc cgt cca gaa caa acc tgc gtt gct 96 Arg Leu Glu His Trp Ala Lys Thr Arg Pro Glu Gln Thr Cys Val Ala 20 25 30 gcc agg gcg gca aat ggg gaa tgg cgt cgt atc agc tac gcg gaa atg 144 Ala Arg Ala Ala Asn Gly Glu Trp Arg Arg Ile Ser Tyr Ala Glu Met 35 40 45 ttc cac aac gtc cgc gcc atc gca cag agc ttg ctt cct tac gga cta 192 Phe His Asn Val Arg Ala Ile Ala Gln Ser Leu Leu Pro Tyr Gly Leu 50 55 60 tcg gca gag cgt ccg ctg ctt atc gtc tct gga aat gac ctg gaa cat 240 Ser Ala Glu Arg Pro Leu Leu Ile Val Ser Gly Asn Asp Leu Glu His 65 70 75 80 ctt cag ctg gca ttt ggg gct atg tat gcg ggc att ccc tat tgc ccg 288 Leu Gln Leu Ala Phe Gly Ala Met Tyr Ala Gly Ile Pro Tyr Cys Pro 85 90 95 gtg tct cct gct tat tca ctg ctg tcg caa gat ttg gcg aag ctg cgt 336 Val Ser Pro Ala Tyr Ser Leu Leu Ser Gln Asp Leu Ala Lys Leu Arg 100 105 110 cac atc gta ggt ctt ctg caa ccg gga ctg gtc ttt gct gcc gat gca 384 His Ile Val Gly Leu Leu Gln Pro Gly Leu Val Phe Ala Ala Asp Ala 115 120 125 gca cct ttc cag cgc gca att gag acc att ctg ccg gac gac gtg ccc 432 Ala Pro Phe Gln Arg Ala Ile Glu Thr Ile Leu Pro Asp Asp Val Pro 130 135 140 gca atc ttc act cga ggc gaa ttg gcc ggg cgg cgc acg gtg agt ttt 480 Ala Ile Phe Thr Arg Gly Glu Leu Ala Gly Arg Arg Thr Val Ser Phe 145 150 155 160 gac agc ctg ctg gag cag cct ggt ggg att gag gca gat aat gcc ttt 528 Asp Ser Leu Leu Glu Gln Pro Gly Gly Ile Glu Ala Asp Asn Ala Phe 165 170 175 gcg gca act ggc ccc gat acg att gcc aag ttc ttg ttc act tct ggc 576 Ala Ala Thr Gly Pro Asp Thr Ile Ala Lys Phe Leu Phe Thr Ser Gly 180 185 190 tct acc aaa ctg cct aag gcg gtg ccg act act cag cga atg ctc tgc 624 Ser Thr Lys Leu Pro Lys Ala Val Pro Thr Thr Gln Arg Met Leu Cys 195 200 205 gcc aat cag cag atg ctt ctg caa act ttc ccg gtt ttt ggt gaa gag 672 Ala Asn Gln Gln Met Leu Leu Gln Thr Phe Pro Val Phe Gly Glu Glu 210 215 220 ccg ccg gtg ctg gtg gac tgg ttg ccg tgg aac cac acc ttc ggc ggc 720 Pro Pro Val Leu Val Asp Trp Leu Pro Trp Asn His Thr Phe Gly Gly 225 230 235 240 agc cac aac atc ggc atc gtg ttg tac aac ggc ggc acg tac tac ctt 768 Ser His Asn Ile Gly Ile Val Leu Tyr Asn Gly Gly Thr Tyr Tyr Leu 245 250 255 gac gac ggt aaa cca acc gcc caa ggg ttc gcc gag acg ctt cgc aac 816 Asp Asp Gly Lys Pro Thr Ala Gln Gly Phe Ala Glu Thr Leu Arg Asn 260 265 270 ttg agc gaa atc tct ccc act gcg tac ctc act gtg ccg aaa ggc tgg 864 Leu Ser Glu Ile Ser Pro Thr Ala Tyr Leu Thr Val Pro Lys Gly Trp 275 280 285 gag gaa tta gtg ggt gcc ctt gag cga gac agt acc ctg cgc gaa cgc 912 Glu Glu Leu Val Gly Ala Leu Glu Arg Asp Ser Thr Leu Arg Glu Arg 290 295 300 ttc ttc gct cgc atg aag ctg ttc ttc ttc gcg gcg gct ggg ttg tcg 960 Phe Phe Ala Arg Met Lys Leu Phe Phe Phe Ala Ala Ala Gly Leu Ser 305 310 315 320 caa ggg atc tgg gat cgt ttg gac cgg gtc gct gaa cag cac tgt ggt 1008 Gln Gly Ile Trp Asp Arg Leu Asp Arg Val Ala Glu Gln His Cys Gly 325 330 335 gag cgc att cgc atg atg gcg ggt ctg ggc atg acg gag act gct cct 1056 Glu Arg Ile Arg Met Met Ala Gly Leu Gly Met Thr Glu Thr Ala Pro 340 345 350 tcc tgc act ttt acc acc gga ccg ctg tcg atg gct ggt tac att ggg 1104 Ser Cys Thr Phe Thr Thr Gly Pro Leu Ser Met Ala Gly Tyr Ile Gly 355 360 365 ctg cca gcg cct ggc tgc gag gtc aag ctc gtt ccg gtc gat ggg aaa 1152 Leu Pro Ala Pro Gly Cys Glu Val Lys Leu Val Pro Val Asp Gly Lys 370 375 380 ttg gaa ggg cgt ttc cat ggt ccg cac gtc atg agc ggc tac tgg cgt 1200 Leu Glu Gly Arg Phe His Gly Pro His Val Met Ser Gly Tyr Trp Arg 385 390 395 400 gct cct gaa caa aat gcc caa gcg ttc gac gag gaa ggc tat tac tgc 1248 Ala Pro Glu Gln Asn Ala Gln Ala Phe Asp Glu Glu Gly Tyr Tyr Cys 405 410 415 tcc ggt gat gcc atc aaa ttg gca gat cct gcc gat cct cag aaa ggt 1296 Ser Gly Asp Ala Ile Lys Leu Ala Asp Pro Ala Asp Pro Gln Lys Gly 420 425 430 ctg atg ttt gac ggt cga att gct gaa gac ttc aag ctg tcc tca ggg 1344 Leu Met Phe Asp Gly Arg Ile Ala Glu Asp Phe Lys Leu Ser Ser Gly 435 440 445 gta ttt gtc agc gtt ggg cca ttg cgc acg cgg gcg gtt ctg gaa ggc 1392 Val Phe Val Ser Val Gly Pro Leu Arg Thr Arg Ala Val Leu Glu Gly 450 455 460 ggc tct tac gtc ctg gac gta gtg gtt gct gct cct gat cgt gaa tgc 1440 Gly Ser Tyr Val Leu Asp Val Val Val Ala Ala Pro Asp Arg Glu Cys 465 470 475 480 ctt gga ttg ctc gtg ttt ccg cgt ctt ctc gac tgc cgt gcc ttg tcg 1488 Leu Gly Leu Leu Val Phe Pro Arg Leu Leu Asp Cys Arg Ala Leu Ser 485 490 495 ggg cta gga aaa gag gcg tcg gac gcc gag gtg ctt gcc agt gag ccg 1536 Gly Leu Gly Lys Glu Ala Ser Asp Ala Glu Val Leu Ala Ser Glu Pro 500 505 510 gtt cgg gcc tgg ttt gct gac tgg ctc aaa cga ctc aat cga gaa gca 1584 Val Arg Ala Trp Phe Ala Asp Trp Leu Lys Arg Leu Asn Arg Glu Ala 515 520 525 act ggc aat gcc agt cgc atc atg tgg gta ggg ctc ctc gat acg ccg 1632 Thr Gly Asn Ala Ser Arg Ile Met Trp Val Gly Leu Leu Asp Thr Pro 530 535 540 ccg tcg att gat aag ggc gag gtc act gac aag ggc tcg atc aac cag 1680 Pro Ser Ile Asp Lys Gly Glu Val Thr Asp Lys Gly Ser Ile Asn Gln 545 550 555 560 cgc gct gtt ttg caa tgg cgg tcg gcg aaa gtt gat gcg ctg tat cgt 1728 Arg Ala Val Leu Gln Trp Arg Ser Ala Lys Val Asp Ala Leu Tyr Arg 565 570 575 ggt gaa gat caa tcc atg ctg cgt gac gag gcc aca ctg tga 1770 Gly Glu Asp Gln Ser Met Leu Arg Asp Glu Ala Thr Leu 580 585 30 589 PRT Pseudomonas sp. 30 Met Arg Ser Leu Glu Ala Leu Leu Pro Phe Pro Gly Arg Ile Leu Glu 1 5 10 15 Arg Leu Glu His Trp Ala Lys Thr Arg Pro Glu Gln Thr Cys Val Ala 20 25 30 Ala Arg Ala Ala Asn Gly Glu Trp Arg Arg Ile Ser Tyr Ala Glu Met 35 40 45 Phe His Asn Val Arg Ala Ile Ala Gln Ser Leu Leu Pro Tyr Gly Leu 50 55 60 Ser Ala Glu Arg Pro Leu Leu Ile Val Ser Gly Asn Asp Leu Glu His 65 70 75 80 Leu Gln Leu Ala Phe Gly Ala Met Tyr Ala Gly Ile Pro Tyr Cys Pro 85 90 95 Val Ser Pro Ala Tyr Ser Leu Leu Ser Gln Asp Leu Ala Lys Leu Arg 100 105 110 His Ile Val Gly Leu Leu Gln Pro Gly Leu Val Phe Ala Ala Asp Ala 115 120 125 Ala Pro Phe Gln Arg Ala Ile Glu Thr Ile Leu Pro Asp Asp Val Pro 130 135 140 Ala Ile Phe Thr Arg Gly Glu Leu Ala Gly Arg Arg Thr Val Ser Phe 145 150 155 160 Asp Ser Leu Leu Glu Gln Pro Gly Gly Ile Glu Ala Asp Asn Ala Phe 165 170 175 Ala Ala Thr Gly Pro Asp Thr Ile Ala Lys Phe Leu Phe Thr Ser Gly 180 185 190 Ser Thr Lys Leu Pro Lys Ala Val Pro Thr Thr Gln Arg Met Leu Cys 195 200 205 Ala Asn Gln Gln Met Leu Leu Gln Thr Phe Pro Val Phe Gly Glu Glu 210 215 220 Pro Pro Val Leu Val Asp Trp Leu Pro Trp Asn His Thr Phe Gly Gly 225 230 235 240 Ser His Asn Ile Gly Ile Val Leu Tyr Asn Gly Gly Thr Tyr Tyr Leu 245 250 255 Asp Asp Gly Lys Pro Thr Ala Gln Gly Phe Ala Glu Thr Leu Arg Asn 260 265 270 Leu Ser Glu Ile Ser Pro Thr Ala Tyr Leu Thr Val Pro Lys Gly Trp 275 280 285 Glu Glu Leu Val Gly Ala Leu Glu Arg Asp Ser Thr Leu Arg Glu Arg 290 295 300 Phe Phe Ala Arg Met Lys Leu Phe Phe Phe Ala Ala Ala Gly Leu Ser 305 310 315 320 Gln Gly Ile Trp Asp Arg Leu Asp Arg Val Ala Glu Gln His Cys Gly 325 330 335 Glu Arg Ile Arg Met Met Ala Gly Leu Gly Met Thr Glu Thr Ala Pro 340 345 350 Ser Cys Thr Phe Thr Thr Gly Pro Leu Ser Met Ala Gly Tyr Ile Gly 355 360 365 Leu Pro Ala Pro Gly Cys Glu Val Lys Leu Val Pro Val Asp Gly Lys 370 375 380 Leu Glu Gly Arg Phe His Gly Pro His Val Met Ser Gly Tyr Trp Arg 385 390 395 400 Ala Pro Glu Gln Asn Ala Gln Ala Phe Asp Glu Glu Gly Tyr Tyr Cys 405 410 415 Ser Gly Asp Ala Ile Lys Leu Ala Asp Pro Ala Asp Pro Gln Lys Gly 420 425 430 Leu Met Phe Asp Gly Arg Ile Ala Glu Asp Phe Lys Leu Ser Ser Gly 435 440 445 Val Phe Val Ser Val Gly Pro Leu Arg Thr Arg Ala Val Leu Glu Gly 450 455 460 Gly Ser Tyr Val Leu Asp Val Val Val Ala Ala Pro Asp Arg Glu Cys 465 470 475 480 Leu Gly Leu Leu Val Phe Pro Arg Leu Leu Asp Cys Arg Ala Leu Ser 485 490 495 Gly Leu Gly Lys Glu Ala Ser Asp Ala Glu Val Leu Ala Ser Glu Pro 500 505 510 Val Arg Ala Trp Phe Ala Asp Trp Leu Lys Arg Leu Asn Arg Glu Ala 515 520 525 Thr Gly Asn Ala Ser Arg Ile Met Trp Val Gly Leu Leu Asp Thr Pro 530 535 540 Pro Ser Ile Asp Lys Gly Glu Val Thr Asp Lys Gly Ser Ile Asn Gln 545 550 555 560 Arg Ala Val Leu Gln Trp Arg Ser Ala Lys Val Asp Ala Leu Tyr Arg 565 570 575 Gly Glu Asp Gln Ser Met Leu Arg Asp Glu Ala Thr Leu 580 585 31 1296 DNA Pseudomonas sp. CDS (1)..(1293) product = ”beta-Ketothiolase“ / gene = ”aat“ 31 atg agt tgg tca ggg ggg gct tac tcg gcg ttt tcc gac act gcg ttg 48 Met Ser Trp Ser Gly Gly Ala Tyr Ser Ala Phe Ser Asp Thr Ala Leu 1 5 10 15 gtt gcg gca gtg cgc acc ccc tgg att gat tgc ggg ggt gcc ctg tcg 96 Val Ala Ala Val Arg Thr Pro Trp Ile Asp Cys Gly Gly Ala Leu Ser 20 25 30 ctg gtg tcg cct atc gac tta ggg gta aag gtc gct cgc gaa gtt ctg 144 Leu Val Ser Pro Ile Asp Leu Gly Val Lys Val Ala Arg Glu Val Leu 35 40 45 atg cgt gcg tcg ctt gaa cca caa atg gtc gat agc gta ctc gca ggc 192 Met Arg Ala Ser Leu Glu Pro Gln Met Val Asp Ser Val Leu Ala Gly 50 55 60 tct atg gct caa gca agc ttt gat gct tac ctg ctc ccg cgg cac att 240 Ser Met Ala Gln Ala Ser Phe Asp Ala Tyr Leu Leu Pro Arg His Ile 65 70 75 80 ggc ttg tac agc ggt gtt ccc aag tcg gtt ccg gcc ttg ggg gtg cag 288 Gly Leu Tyr Ser Gly Val Pro Lys Ser Val Pro Ala Leu Gly Val Gln 85 90 95 cgc att tgc ggc aca ggc ttc gaa ctg ctt cgg cag gcc ggc gag cag 336 Arg Ile Cys Gly Thr Gly Phe Glu Leu Leu Arg Gln Ala Gly Glu Gln 100 105 110 att tcc caa ggc gct gat cac gtg ctg tgt gtc gcg gca gag tcc atg 384 Ile Ser Gln Gly Ala Asp His Val Leu Cys Val Ala Ala Glu Ser Met 115 120 125 tcg cgt aac ccc atc gcg tcg tat aca cac cgg ggc ggg ttc cgc ctc 432 Ser Arg Asn Pro Ile Ala Ser Tyr Thr His Arg Gly Gly Phe Arg Leu 130 135 140 ggt gcg ccc gtt gag ttc aag gat ttt ttg tgg gag gca ttg ttt gat 480 Gly Ala Pro Val Glu Phe Lys Asp Phe Leu Trp Glu Ala Leu Phe Asp 145 150 155 160 cct gct cca gga ctc gac atg atc gct acc gca gaa aac ctg gcg cgc 528 Pro Ala Pro Gly Leu Asp Met Ile Ala Thr Ala Glu Asn Leu Ala Arg 165 170 175 ctg tac gga atc acc agg gga gaa gct aat tcc tac gcg gta agc agc 576 Leu Tyr Gly Ile Thr Arg Gly Glu Ala Asn Ser Tyr Ala Val Ser Ser 180 185 190 ttc gag cgc gca ttg agg gcg caa gag gag aaa tgg att gac caa gag 624 Phe Glu Arg Ala Leu Arg Ala Gln Glu Glu Lys Trp Ile Asp Gln Glu 195 200 205 atc gtg gct gtt acg gat gaa cag ttc gat tta gag ggc tac aac agt 672 Ile Val Ala Val Thr Asp Glu Gln Phe Asp Leu Glu Gly Tyr Asn Ser 210 215 220 cga gca att gaa ctg cct cgg aag gca aaa ttg ttg atc gtg aca gtc 720 Arg Ala Ile Glu Leu Pro Arg Lys Ala Lys Leu Leu Ile Val Thr Val 225 230 235 240 atc cgc ggc cta gca gtc ttt gaa gcc ctt tcc cga ttg aag cct gtt 768 Ile Arg Gly Leu Ala Val Phe Glu Ala Leu Ser Arg Leu Lys Pro Val 245 250 255 cat tct ggc ggg gtg cag act gcg ggc aac agc tgt gcc gta gtg gac 816 His Ser Gly Gly Val Gln Thr Ala Gly Asn Ser Cys Ala Val Val Asp 260 265 270 ggc gcc gcg gcg gct ttg gtg gct cga gag tcg tct gcg aca cag ccg 864 Gly Ala Ala Ala Ala Leu Val Ala Arg Glu Ser Ser Ala Thr Gln Pro 275 280 285 gtc ttg gct agg ata ctg gct acc tcc gta gtc ggg atc gag ccc gag 912 Val Leu Ala Arg Ile Leu Ala Thr Ser Val Val Gly Ile Glu Pro Glu 290 295 300 cat atg ggg ctc ggc cct gcg ccc gcg att cgc ctg ctg ctt gcg cgt 960 His Met Gly Leu Gly Pro Ala Pro Ala Ile Arg Leu Leu Leu Ala Arg 305 310 315 320 agt gat ctt agt ttg agg gat atc gac ctc ttt gag ata aac gag gcg 1008 Ser Asp Leu Ser Leu Arg Asp Ile Asp Leu Phe Glu Ile Asn Glu Ala 325 330 335 cag gcc gcc caa gtt cta gcg gta cag cat gaa ttg ggt att gag cac 1056 Gln Ala Ala Gln Val Leu Ala Val Gln His Glu Leu Gly Ile Glu His 340 345 350 tca aaa ctt aat att tgg ggc ggg gcc att gca ctt gga cac ccg ctt 1104 Ser Lys Leu Asn Ile Trp Gly Gly Ala Ile Ala Leu Gly His Pro Leu 355 360 365 gcc gcg acc gga ttg cgt ctc tgc atg acc ctc gct cac caa ttg caa 1152 Ala Ala Thr Gly Leu Arg Leu Cys Met Thr Leu Ala His Gln Leu Gln 370 375 380 gct aat aac ttt cga tat gga att gcc tcg gca tgc att ggt ggg gga 1200 Ala Asn Asn Phe Arg Tyr Gly Ile Ala Ser Ala Cys Ile Gly Gly Gly 385 390 395 400 cag ggg atg gcg gtt ctt tta gag aat ccc cac ttc ggt tcg tcc tct 1248 Gln Gly Met Ala Val Leu Leu Glu Asn Pro His Phe Gly Ser Ser Ser 405 410 415 gca cga agt tcg atg att aac aga gtt gac cac tat cca ctg agc taa 1296 Ala Arg Ser Ser Met Ile Asn Arg Val Asp His Tyr Pro Leu Ser 420 425 430 32 431 PRT Pseudomonas sp. 32 Met Ser Trp Ser Gly Gly Ala Tyr Ser Ala Phe Ser Asp Thr Ala Leu 1 5 10 15 Val Ala Ala Val Arg Thr Pro Trp Ile Asp Cys Gly Gly Ala Leu Ser 20 25 30 Leu Val Ser Pro Ile Asp Leu Gly Val Lys Val Ala Arg Glu Val Leu 35 40 45 Met Arg Ala Ser Leu Glu Pro Gln Met Val Asp Ser Val Leu Ala Gly 50 55 60 Ser Met Ala Gln Ala Ser Phe Asp Ala Tyr Leu Leu Pro Arg His Ile 65 70 75 80 Gly Leu Tyr Ser Gly Val Pro Lys Ser Val Pro Ala Leu Gly Val Gln 85 90 95 Arg Ile Cys Gly Thr Gly Phe Glu Leu Leu Arg Gln Ala Gly Glu Gln 100 105 110 Ile Ser Gln Gly Ala Asp His Val Leu Cys Val Ala Ala Glu Ser Met 115 120 125 Ser Arg Asn Pro Ile Ala Ser Tyr Thr His Arg Gly Gly Phe Arg Leu 130 135 140 Gly Ala Pro Val Glu Phe Lys Asp Phe Leu Trp Glu Ala Leu Phe Asp 145 150 155 160 Pro Ala Pro Gly Leu Asp Met Ile Ala Thr Ala Glu Asn Leu Ala Arg 165 170 175 Leu Tyr Gly Ile Thr Arg Gly Glu Ala Asn Ser Tyr Ala Val Ser Ser 180 185 190 Phe Glu Arg Ala Leu Arg Ala Gln Glu Glu Lys Trp Ile Asp Gln Glu 195 200 205 Ile Val Ala Val Thr Asp Glu Gln Phe Asp Leu Glu Gly Tyr Asn Ser 210 215 220 Arg Ala Ile Glu Leu Pro Arg Lys Ala Lys Leu Leu Ile Val Thr Val 225 230 235 240 Ile Arg Gly Leu Ala Val Phe Glu Ala Leu Ser Arg Leu Lys Pro Val 245 250 255 His Ser Gly Gly Val Gln Thr Ala Gly Asn Ser Cys Ala Val Val Asp 260 265 270 Gly Ala Ala Ala Ala Leu Val Ala Arg Glu Ser Ser Ala Thr Gln Pro 275 280 285 Val Leu Ala Arg Ile Leu Ala Thr Ser Val Val Gly Ile Glu Pro Glu 290 295 300 His Met Gly Leu Gly Pro Ala Pro Ala Ile Arg Leu Leu Leu Ala Arg 305 310 315 320 Ser Asp Leu Ser Leu Arg Asp Ile Asp Leu Phe Glu Ile Asn Glu Ala 325 330 335 Gln Ala Ala Gln Val Leu Ala Val Gln His Glu Leu Gly Ile Glu His 340 345 350 Ser Lys Leu Asn Ile Trp Gly Gly Ala Ile Ala Leu Gly His Pro Leu 355 360 365 Ala Ala Thr Gly Leu Arg Leu Cys Met Thr Leu Ala His Gln Leu Gln 370 375 380 Ala Asn Asn Phe Arg Tyr Gly Ile Ala Ser Ala Cys Ile Gly Gly Gly 385 390 395 400 Gln Gly Met Ala Val Leu Leu Glu Asn Pro His Phe Gly Ser Ser Ser 405 410 415 Ala Arg Ser Ser Met Ile Asn Arg Val Asp His Tyr Pro Leu Ser 420 425 430 33 1596 DNA Pseudomonas sp. CDS (1)..(1593) product = ”Chemotaxis-Protein“ / gene = ”mac“ 33 atg att agt ttc gct cgt atg gca gaa agt tta gga gtc cag gct aaa 48 Met Ile Ser Phe Ala Arg Met Ala Glu Ser Leu Gly Val Gln Ala Lys 1 5 10 15 ctt gcc ctt gcc ttc gca ctc gta tta tgt gtc ggg ctg att gtt acc 96 Leu Ala Leu Ala Phe Ala Leu Val Leu Cys Val Gly Leu Ile Val Thr 20 25 30 ggc acg ggt ttc tac agt gta cat acc ttg tca ggg ttg gtg gaa aag 144 Gly Thr Gly Phe Tyr Ser Val His Thr Leu Ser Gly Leu Val Glu Lys 35 40 45 agc gcg ata gct ggt gag ttg cgg gcg aaa att cag gaa ctg aag gtt 192 Ser Ala Ile Ala Gly Glu Leu Arg Ala Lys Ile Gln Glu Leu Lys Val 50 55 60 ctg gag cag cgc gcc tta ttc atc gcc gat gaa ggg tcg ctg aag cag 240 Leu Glu Gln Arg Ala Leu Phe Ile Ala Asp Glu Gly Ser Leu Lys Gln 65 70 75 80 cgc tcg atc ctc cta agt cag gtg ata gct gaa gtt aat gat gct ata 288 Arg Ser Ile Leu Leu Ser Gln Val Ile Ala Glu Val Asn Asp Ala Ile 85 90 95 gat att ttt gac ttt cag cgc gga cga tct gag tta ctt aaa ttc gct 336 Asp Ile Phe Asp Phe Gln Arg Gly Arg Ser Glu Leu Leu Lys Phe Ala 100 105 110 gct tct tcg cgc gaa gca agt tac tcc att gag gtc ggt agt aac gct 384 Ala Ser Ser Arg Glu Ala Ser Tyr Ser Ile Glu Val Gly Ser Asn Ala 115 120 125 gcg gcc gat aag ttg cag tcg ggc gaa cca agt gac gca ttg atg gtt 432 Ala Ala Asp Lys Leu Gln Ser Gly Glu Pro Ser Asp Ala Leu Met Val 130 135 140 gcc gat aaa aag ctg aat gtt gag tat gag caa ttg agt tct gct gtg 480 Ala Asp Lys Lys Leu Asn Val Glu Tyr Glu Gln Leu Ser Ser Ala Val 145 150 155 160 aat gca ctg atg ggg cat tta att gag gat cag aat gaa aaa gtt cca 528 Asn Ala Leu Met Gly His Leu Ile Glu Asp Gln Asn Glu Lys Val Pro 165 170 175 cta atc tac tat atg ctt ggc ggc gta act ttg ttt acg atg ctc atg 576 Leu Ile Tyr Tyr Met Leu Gly Gly Val Thr Leu Phe Thr Met Leu Met 180 185 190 agt gct tat tcg gtc tgg ttc att tcg cgt cag tta gtt ccg cca tta 624 Ser Ala Tyr Ser Val Trp Phe Ile Ser Arg Gln Leu Val Pro Pro Leu 195 200 205 aag tcg acg gtg cag ctt gcc gag cgg att gca tca ggc gac ttg gct 672 Lys Ser Thr Val Gln Leu Ala Glu Arg Ile Ala Ser Gly Asp Leu Ala 210 215 220 gat gtc ggg gac agc agg cgc aag gat gaa atc ggt cag ttg caa agt 720 Asp Val Gly Asp Ser Arg Arg Lys Asp Glu Ile Gly Gln Leu Gln Ser 225 230 235 240 gca act agg cgg atg gcg att gga ctg cgt aat ctg gtc ggt gat att 768 Ala Thr Arg Arg Met Ala Ile Gly Leu Arg Asn Leu Val Gly Asp Ile 245 250 255 ggt caa agt cgt gcg caa ctg gtt tca tcg tcc agc gac ctt tcg gcc 816 Gly Gln Ser Arg Ala Gln Leu Val Ser Ser Ser Ser Asp Leu Ser Ala 260 265 270 atc tgt gct cag gct cag att gat gtc gag tgc cag aag ctt tcg gtc 864 Ile Cys Ala Gln Ala Gln Ile Asp Val Glu Cys Gln Lys Leu Ser Val 275 280 285 gcc cag gtc tct acc gcc gtg aac gag ttg gtt gaa acc gtc cag gca 912 Ala Gln Val Ser Thr Ala Val Asn Glu Leu Val Glu Thr Val Gln Ala 290 295 300 ata gca aaa agc acc gaa gag gca gca aca gtc gcc gtc ttg gcc gat 960 Ile Ala Lys Ser Thr Glu Glu Ala Ala Thr Val Ala Val Leu Ala Asp 305 310 315 320 gaa aag gca cgc ggt ggt gaa agt gtc gtt aac aag gcc gtt gat ttc 1008 Glu Lys Ala Arg Gly Gly Glu Ser Val Val Asn Lys Ala Val Asp Phe 325 330 335 att gag cac ctc tcc gga gat atg gcg gaa ctg gga gac gca atg gag 1056 Ile Glu His Leu Ser Gly Asp Met Ala Glu Leu Gly Asp Ala Met Glu 340 345 350 cgg ctt cag aac gac agt gcg cag atc aat aag gta gta gac gtc att 1104 Arg Leu Gln Asn Asp Ser Ala Gln Ile Asn Lys Val Val Asp Val Ile 355 360 365 aag gct gtg gcg gag cag acc aat ctg cta gcc ctg aat gcg gcg ata 1152 Lys Ala Val Ala Glu Gln Thr Asn Leu Leu Ala Leu Asn Ala Ala Ile 370 375 380 gag gcg gcc cgt gca gga gag cag ggc agg ggc ttt gcg gtc gtg gcg 1200 Glu Ala Ala Arg Ala Gly Glu Gln Gly Arg Gly Phe Ala Val Val Ala 385 390 395 400 gat gag gtt cgt gct ttg gcg atg cgc acc caa caa tcg acc aaa gaa 1248 Asp Glu Val Arg Ala Leu Ala Met Arg Thr Gln Gln Ser Thr Lys Glu 405 410 415 att gag agg cta gtg gtt tca ttg cag cag gga agt gaa gct gcg ggc 1296 Ile Glu Arg Leu Val Val Ser Leu Gln Gln Gly Ser Glu Ala Ala Gly 420 425 430 gag ttg atg cgg cgt ggc aag gtc cgg acg cat gac gtc gtt gga ttg 1344 Glu Leu Met Arg Arg Gly Lys Val Arg Thr His Asp Val Val Gly Leu 435 440 445 gcc cag caa gcc gcg cgc cgc gct act cga aat tac cca gct gtc gcc 1392 Ala Gln Gln Ala Ala Arg Arg Ala Thr Arg Asn Tyr Pro Ala Val Ala 450 455 460 ggc atc caa gcg atg aac tat cag atc gcc gct gga gca gag cag caa 1440 Gly Ile Gln Ala Met Asn Tyr Gln Ile Ala Ala Gly Ala Glu Gln Gln 465 470 475 480 ggg gct gct gtg gtt caa atc aac cag aat atg ctt gaa gtg cat aag 1488 Gly Ala Ala Val Val Gln Ile Asn Gln Asn Met Leu Glu Val His Lys 485 490 495 atg gct gac gag tcc gcc att aaa gcg gga cag acc atg aag tca tcg 1536 Met Ala Asp Glu Ser Ala Ile Lys Ala Gly Gln Thr Met Lys Ser Ser 500 505 510 aag gag ctt gct cac ctc ggc agt gcg cta caa aaa tcc gtt gat cga 1584 Lys Glu Leu Ala His Leu Gly Ser Ala Leu Gln Lys Ser Val Asp Arg 515 520 525 ttc cag ctg tag 1596 Phe Gln Leu 530 34 531 PRT Pseudomonas sp. 34 Met Ile Ser Phe Ala Arg Met Ala Glu Ser Leu Gly Val Gln Ala Lys 1 5 10 15 Leu Ala Leu Ala Phe Ala Leu Val Leu Cys Val Gly Leu Ile Val Thr 20 25 30 Gly Thr Gly Phe Tyr Ser Val His Thr Leu Ser Gly Leu Val Glu Lys 35 40 45 Ser Ala Ile Ala Gly Glu Leu Arg Ala Lys Ile Gln Glu Leu Lys Val 50 55 60 Leu Glu Gln Arg Ala Leu Phe Ile Ala Asp Glu Gly Ser Leu Lys Gln 65 70 75 80 Arg Ser Ile Leu Leu Ser Gln Val Ile Ala Glu Val Asn Asp Ala Ile 85 90 95 Asp Ile Phe Asp Phe Gln Arg Gly Arg Ser Glu Leu Leu Lys Phe Ala 100 105 110 Ala Ser Ser Arg Glu Ala Ser Tyr Ser Ile Glu Val Gly Ser Asn Ala 115 120 125 Ala Ala Asp Lys Leu Gln Ser Gly Glu Pro Ser Asp Ala Leu Met Val 130 135 140 Ala Asp Lys Lys Leu Asn Val Glu Tyr Glu Gln Leu Ser Ser Ala Val 145 150 155 160 Asn Ala Leu Met Gly His Leu Ile Glu Asp Gln Asn Glu Lys Val Pro 165 170 175 Leu Ile Tyr Tyr Met Leu Gly Gly Val Thr Leu Phe Thr Met Leu Met 180 185 190 Ser Ala Tyr Ser Val Trp Phe Ile Ser Arg Gln Leu Val Pro Pro Leu 195 200 205 Lys Ser Thr Val Gln Leu Ala Glu Arg Ile Ala Ser Gly Asp Leu Ala 210 215 220 Asp Val Gly Asp Ser Arg Arg Lys Asp Glu Ile Gly Gln Leu Gln Ser 225 230 235 240 Ala Thr Arg Arg Met Ala Ile Gly Leu Arg Asn Leu Val Gly Asp Ile 245 250 255 Gly Gln Ser Arg Ala Gln Leu Val Ser Ser Ser Ser Asp Leu Ser Ala 260 265 270 Ile Cys Ala Gln Ala Gln Ile Asp Val Glu Cys Gln Lys Leu Ser Val 275 280 285 Ala Gln Val Ser Thr Ala Val Asn Glu Leu Val Glu Thr Val Gln Ala 290 295 300 Ile Ala Lys Ser Thr Glu Glu Ala Ala Thr Val Ala Val Leu Ala Asp 305 310 315 320 Glu Lys Ala Arg Gly Gly Glu Ser Val Val Asn Lys Ala Val Asp Phe 325 330 335 Ile Glu His Leu Ser Gly Asp Met Ala Glu Leu Gly Asp Ala Met Glu 340 345 350 Arg Leu Gln Asn Asp Ser Ala Gln Ile Asn Lys Val Val Asp Val Ile 355 360 365 Lys Ala Val Ala Glu Gln Thr Asn Leu Leu Ala Leu Asn Ala Ala Ile 370 375 380 Glu Ala Ala Arg Ala Gly Glu Gln Gly Arg Gly Phe Ala Val Val Ala 385 390 395 400 Asp Glu Val Arg Ala Leu Ala Met Arg Thr Gln Gln Ser Thr Lys Glu 405 410 415 Ile Glu Arg Leu Val Val Ser Leu Gln Gln Gly Ser Glu Ala Ala Gly 420 425 430 Glu Leu Met Arg Arg Gly Lys Val Arg Thr His Asp Val Val Gly Leu 435 440 445 Ala Gln Gln Ala Ala Arg Arg Ala Thr Arg Asn Tyr Pro Ala Val Ala 450 455 460 Gly Ile Gln Ala Met Asn Tyr Gln Ile Ala Ala Gly Ala Glu Gln Gln 465 470 475 480 Gly Ala Ala Val Val Gln Ile Asn Gln Asn Met Leu Glu Val His Lys 485 490 495 Met Ala Asp Glu Ser Ala Ile Lys Ala Gly Gln Thr Met Lys Ser Ser 500 505 510 Lys Glu Leu Ala His Leu Gly Ser Ala Leu Gln Lys Ser Val Asp Arg 515 520 525 Phe Gln Leu 530 35 411 DNA Pseudomonas sp. 35 ctagcctaac tgttgcgctt caggctccgc atggatcttg tgcagcagca atagcaattg 60 ttcacgttcg tcatcactca gcatcgacgt cgcgtcttgg tcgctctgta ccacgatctt 120 cttcagctct ttgagctgcg tctccccagc tttgctgaga aatatcccat aggaacgctt 180 gtccggcttg cagcgcacgc gcacagcaag gccgagcttc tcgagcttgt tcagcaaggg 240 aaccagttgt ggtggttcga ttgcgagcat ccgcgctagg tcagcctgca taagcccagg 300 gctcgcttcg atgattagaa gtgccgacag ctgcgccggg cgtaggtcat atggcgtcag 360 ggcttcaatc aggccctgag cgagcttcag ctgtgagccg gcgtaaggca t 411 36 136 PRT Pseudomonas sp. 36 Met Pro Tyr Ala Gly Ser Gln Leu Lys Leu Ala Gln Gly Leu Ile Glu 1 5 10 15 Ala Leu Thr Pro Tyr Asp Leu Arg Pro Ala Gln Leu Ser Ala Leu Leu 20 25 30 Ile Ile Glu Ala Ser Pro Gly Leu Met Gln Ala Asp Leu Ala Arg Met 35 40 45 Leu Ala Ile Glu Pro Pro Gln Leu Val Pro Leu Leu Asn Lys Leu Glu 50 55 60 Lys Leu Gly Leu Ala Val Arg Val Arg Cys Lys Pro Asp Lys Arg Ser 65 70 75 80 Tyr Gly Ile Phe Leu Ser Lys Ala Gly Glu Thr Gln Leu Lys Glu Leu 85 90 95 Lys Lys Ile Val Val Gln Ser Asp Gln Asp Ala Thr Ser Met Leu Ser 100 105 110 Asp Asp Glu Arg Glu Gln Leu Leu Leu Leu Leu His Lys Ile His Ala 115 120 125 Glu Pro Glu Ala Gln Gln Leu Gly 130 135 37 1446 DNA Pseudomonas sp. CDS (1)..(1443) prodict = ”Coniferylaldehyd-Dehydrogenase“ / gene = ”caldh“ 37 atg agc att ctt ggt ttg aat ggt gcc ccg gtc gga gct gag cag ctg 48 Met Ser Ile Leu Gly Leu Asn Gly Ala Pro Val Gly Ala Glu Gln Leu 1 5 10 15 ggc tcg gct ctt gat cgc atg aag aag gcg cac ctg gag cag ggg cct 96 Gly Ser Ala Leu Asp Arg Met Lys Lys Ala His Leu Glu Gln Gly Pro 20 25 30 gca aac ttg gag ctg cgt ctg agt agg ctg gat cgt gcg att gca atg 144 Ala Asn Leu Glu Leu Arg Leu Ser Arg Leu Asp Arg Ala Ile Ala Met 35 40 45 ctt ctg gaa aat cgt gaa gca att gcc gac gcg gtt tct gct gac ttt 192 Leu Leu Glu Asn Arg Glu Ala Ile Ala Asp Ala Val Ser Ala Asp Phe 50 55 60 ggc aat cgc agc cgt gag caa aca ctg ctt tgc gac att gct ggc tcg 240 Gly Asn Arg Ser Arg Glu Gln Thr Leu Leu Cys Asp Ile Ala Gly Ser 65 70 75 80 gtg gca agc ctg aag gat agc cgc gag cac gtg gcc aaa tgg atg gag 288 Val Ala Ser Leu Lys Asp Ser Arg Glu His Val Ala Lys Trp Met Glu 85 90 95 ccc gaa cat cac aag gcg atg ttt cca ggg gcg gag gca cgc gtt gag 336 Pro Glu His His Lys Ala Met Phe Pro Gly Ala Glu Ala Arg Val Glu 100 105 110 ttt cag ccg ctg ggt gtc gtt ggg gtc att agt ccc tgg aac ttc cct 384 Phe Gln Pro Leu Gly Val Val Gly Val Ile Ser Pro Trp Asn Phe Pro 115 120 125 atc gta ctg gcc ttt ggg ccg ctg gcc ggc ata ttc gca gca ggt aat 432 Ile Val Leu Ala Phe Gly Pro Leu Ala Gly Ile Phe Ala Ala Gly Asn 130 135 140 cgc gcc atg ctc aag ccg tcc gag ctt acc ccg cgg act tct gcc ctg 480 Arg Ala Met Leu Lys Pro Ser Glu Leu Thr Pro Arg Thr Ser Ala Leu 145 150 155 160 ctt gcg gag cta att gct cgt tac ttc gat gaa act gag ctg act aca 528 Leu Ala Glu Leu Ile Ala Arg Tyr Phe Asp Glu Thr Glu Leu Thr Thr 165 170 175 gtg ctg ggc gac gct gaa gtc ggt gcg ctg ttc agt gct cag cct ttc 576 Val Leu Gly Asp Ala Glu Val Gly Ala Leu Phe Ser Ala Gln Pro Phe 180 185 190 gat cat ctg atc ttc acc ggc ggc act gcc gtg gcc aag cac atc atg 624 Asp His Leu Ile Phe Thr Gly Gly Thr Ala Val Ala Lys His Ile Met 195 200 205 cgt gcc gcg gcg gat aac cta gtg ccc gtt acc ctg gaa ttg ggt ggc 672 Arg Ala Ala Ala Asp Asn Leu Val Pro Val Thr Leu Glu Leu Gly Gly 210 215 220 aaa tcg ccg gtg atc gtt tcc cgc agt gca gat atg gcg gac gtt gca 720 Lys Ser Pro Val Ile Val Ser Arg Ser Ala Asp Met Ala Asp Val Ala 225 230 235 240 caa cgg gtg ttg acg gtg aaa acc ttc aat gcc ggg caa atc tgt ctg 768 Gln Arg Val Leu Thr Val Lys Thr Phe Asn Ala Gly Gln Ile Cys Leu 245 250 255 gca ccg gac tat gtg ctg ctg ccg gaa gaa tcg ctg gat agc ttt gtc 816 Ala Pro Asp Tyr Val Leu Leu Pro Glu Glu Ser Leu Asp Ser Phe Val 260 265 270 gcc gag gcg acg cgc ttc gtg gcc gca atg tat ccc tcg ctt cta gat 864 Ala Glu Ala Thr Arg Phe Val Ala Ala Met Tyr Pro Ser Leu Leu Asp 275 280 285 aat ccg gat tac acg tcg atc atc aat gcc cga aat ttc gac cgt ctg 912 Asn Pro Asp Tyr Thr Ser Ile Ile Asn Ala Arg Asn Phe Asp Arg Leu 290 295 300 cat cgc tac ctg act gat gcg cag gca aag gga ggg cgc gtc att gaa 960 His Arg Tyr Leu Thr Asp Ala Gln Ala Lys Gly Gly Arg Val Ile Glu 305 310 315 320 atc aat cct gcg gcc gaa gag ttg ggg gat agt ggt atc agg aag atc 1008 Ile Asn Pro Ala Ala Glu Glu Leu Gly Asp Ser Gly Ile Arg Lys Ile 325 330 335 gcg ccc act ttg atc gtg aat gtg tcg gat gaa atg ctg gtc ttg aac 1056 Ala Pro Thr Leu Ile Val Asn Val Ser Asp Glu Met Leu Val Leu Asn 340 345 350 gag gag atc ttt ggt ccg ctg ctc ccg atc aag act tat cgt gat ttc 1104 Glu Glu Ile Phe Gly Pro Leu Leu Pro Ile Lys Thr Tyr Arg Asp Phe 355 360 365 gac tcg gct atc gac tac gtc aac agc aag cag cga cca ctt gcc tcg 1152 Asp Ser Ala Ile Asp Tyr Val Asn Ser Lys Gln Arg Pro Leu Ala Ser 370 375 380 tac ttc ttc ggc gaa gat gcg gtt gag cgt gag caa gtg ctt aag cgt 1200 Tyr Phe Phe Gly Glu Asp Ala Val Glu Arg Glu Gln Val Leu Lys Arg 385 390 395 400 acg gtt tcg ggc gcc gtg gtc gtg aac gat gtc atg agc cat gtg atg 1248 Thr Val Ser Gly Ala Val Val Val Asn Asp Val Met Ser His Val Met 405 410 415 atg gat acg ctt cca ttt ggt ggt gtg ggg cac tcg ggg atg ggg gca 1296 Met Asp Thr Leu Pro Phe Gly Gly Val Gly His Ser Gly Met Gly Ala 420 425 430 tat cac ggc att tat ggt ttc cga acc ttc agc cat gcc aag cct gtt 1344 Tyr His Gly Ile Tyr Gly Phe Arg Thr Phe Ser His Ala Lys Pro Val 435 440 445 ctc gtg caa agt cct gtg ggt gag tcg aac ttg gcg atg cgc gca ccc 1392 Leu Val Gln Ser Pro Val Gly Glu Ser Asn Leu Ala Met Arg Ala Pro 450 455 460 tac gga gaa gcg atc cac gga ctg ctc tct gtc ctc ctt tca acg gag 1440 Tyr Gly Glu Ala Ile His Gly Leu Leu Ser Val Leu Leu Ser Thr Glu 465 470 475 480 tgt tag 1446 Cys 38 481 PRT Pseudomonas sp. 38 Met Ser Ile Leu Gly Leu Asn Gly Ala Pro Val Gly Ala Glu Gln Leu 1 5 10 15 Gly Ser Ala Leu Asp Arg Met Lys Lys Ala His Leu Glu Gln Gly Pro 20 25 30 Ala Asn Leu Glu Leu Arg Leu Ser Arg Leu Asp Arg Ala Ile Ala Met 35 40 45 Leu Leu Glu Asn Arg Glu Ala Ile Ala Asp Ala Val Ser Ala Asp Phe 50 55 60 Gly Asn Arg Ser Arg Glu Gln Thr Leu Leu Cys Asp Ile Ala Gly Ser 65 70 75 80 Val Ala Ser Leu Lys Asp Ser Arg Glu His Val Ala Lys Trp Met Glu 85 90 95 Pro Glu His His Lys Ala Met Phe Pro Gly Ala Glu Ala Arg Val Glu 100 105 110 Phe Gln Pro Leu Gly Val Val Gly Val Ile Ser Pro Trp Asn Phe Pro 115 120 125 Ile Val Leu Ala Phe Gly Pro Leu Ala Gly Ile Phe Ala Ala Gly Asn 130 135 140 Arg Ala Met Leu Lys Pro Ser Glu Leu Thr Pro Arg Thr Ser Ala Leu 145 150 155 160 Leu Ala Glu Leu Ile Ala Arg Tyr Phe Asp Glu Thr Glu Leu Thr Thr 165 170 175 Val Leu Gly Asp Ala Glu Val Gly Ala Leu Phe Ser Ala Gln Pro Phe 180 185 190 Asp His Leu Ile Phe Thr Gly Gly Thr Ala Val Ala Lys His Ile Met 195 200 205 Arg Ala Ala Ala Asp Asn Leu Val Pro Val Thr Leu Glu Leu Gly Gly 210 215 220 Lys Ser Pro Val Ile Val Ser Arg Ser Ala Asp Met Ala Asp Val Ala 225 230 235 240 Gln Arg Val Leu Thr Val Lys Thr Phe Asn Ala Gly Gln Ile Cys Leu 245 250 255 Ala Pro Asp Tyr Val Leu Leu Pro Glu Glu Ser Leu Asp Ser Phe Val 260 265 270 Ala Glu Ala Thr Arg Phe Val Ala Ala Met Tyr Pro Ser Leu Leu Asp 275 280 285 Asn Pro Asp Tyr Thr Ser Ile Ile Asn Ala Arg Asn Phe Asp Arg Leu 290 295 300 His Arg Tyr Leu Thr Asp Ala Gln Ala Lys Gly Gly Arg Val Ile Glu 305 310 315 320 Ile Asn Pro Ala Ala Glu Glu Leu Gly Asp Ser Gly Ile Arg Lys Ile 325 330 335 Ala Pro Thr Leu Ile Val Asn Val Ser Asp Glu Met Leu Val Leu Asn 340 345 350 Glu Glu Ile Phe Gly Pro Leu Leu Pro Ile Lys Thr Tyr Arg Asp Phe 355 360 365 Asp Ser Ala Ile Asp Tyr Val Asn Ser Lys Gln Arg Pro Leu Ala Ser 370 375 380 Tyr Phe Phe Gly Glu Asp Ala Val Glu Arg Glu Gln Val Leu Lys Arg 385 390 395 400 Thr Val Ser Gly Ala Val Val Val Asn Asp Val Met Ser His Val Met 405 410 415 Met Asp Thr Leu Pro Phe Gly Gly Val Gly His Ser Gly Met Gly Ala 420 425 430 Tyr His Gly Ile Tyr Gly Phe Arg Thr Phe Ser His Ala Lys Pro Val 435 440 445 Leu Val Gln Ser Pro Val Gly Glu Ser Asn Leu Ala Met Arg Ala Pro 450 455 460 Tyr Gly Glu Ala Ile His Gly Leu Leu Ser Val Leu Leu Ser Thr Glu 465 470 475 480 Cys 39 1827 DNA Pseudomonas sp. 39 ctatttgtct agtggtcggc gcgaaattcg ataagaaagc tgggcgcgag tgaggccgag 60 ccggcgggca gcttccgaga cattgccttt cacctggccc agagcatggc taatcatcgc 120 gtcctccact tcttgcagcg tcatcgcgct caggtccttt gagtcaagcg gcgagtcgat 180 tgtgctggtc ggtttggaga aggaagtact tgggctgcca gtttcctgtg gctgattatc 240 ttgagcggtg gccaggatgc cgctggcccc aatggagaac atcggttgag tcagtcgttc 300 accgctagtg aagaggtggc tcacgtcaat ggctccatcc tccggagcgc tgatgactcc 360 gcgctccacc aaattttgaa gctcccggat gtttcctgga aagtcgtagc caagcagggc 420 attggctgca cgtggagtga atccgctgac cacccggcta tgacgctgat tgaagcggtg 480 caggaaatag gtcatcagga ggggaatgtc ttccttcctc tctcgaagcg gcgggaggtg 540 gatcgggtaa acattgaggc ggaaaaaaag gtcctcgcgg aactcgccgc gctggacgcc 600 tgcgcgaaga tcgacattgg ttgcggctac cacacggacg tcaaccttga gtgtcctgct 660 tccgccaacc cgttcgacct ccgactcttg cagggcgcga agtaacttcc cttgggccac 720 gaggcttagc gtccctatct cgtcaaggaa tagtgtgccg cccgaagcgc gctcgaaccg 780 tcctgctcga gattgggtgg cgccggtaaa cgccccccgt tcgacgccga acaactcgga 840 ctccatcagg gtttcgggaa tacgtgcgca attgaccgca acaaacgggc cgtcgtgtct 900 ggggctgatg cggtgaagca tgcgggcgaa catctccttg cccacacctg attcacccgt 960 aaacagtacc gtcgcctccg tgggtgctac gcgcttcagc atgtggcagg cagcattgaa 1020 tgccgaggaa attcccacca tgtcgtgttc cgatgcagtg cttgagtctg cggcggagtg 1080 atggggagtg ttcctttgtc cctgctgcgt tcttcgtctc tgcggcgtgc ttggttgccg 1140 acaaatggtt gcgctaagcg ccgccaagtc ctcttcggcg tcttcccatt cttccgctgg 1200 cttgccgatc atgcggcaga tctgcgaacc cgtggagcgg cattccacct ctcggtaaag 1260 gatgaggcga ccaaccagcg cggacgtata gccaatggca taacccgtct gcgtccagca 1320 cgcgggctcg gtgccgatgc cgtagtgcgc aatatgttca tcatcttcgc tcgaatggtg 1380 ccagaggaat tcgccgtagt aggtccccaa atccatgtcg aagtcgaagt ggatcggctc 1440 cacgcgtact gcgccttcca gagagtgcaa gttcgggccg gcggcaaata gggagagcgg 1500 atcggcgttg ctgaagcgct ccttcagaag ggcggcatct ttggcgccgc agtggtaacc 1560 ggttcgcagc atgattccgc gggcgcgggc gaagcccacg ctttcaatta attcgcgtcg 1620 caatgcaccc agtccgctgc tgtggaggag cagcattcgc gcgccgttca accagatgcg 1680 tccatcgcca gggctgaaaa ggagggattc agtgaggtca tgaagggagg ggacggcgcc 1740 tggctccaat tgctcgatgg cgccgcgatt gagtgtcttg ggcgcggtct tggagagttc 1800 ggctagggag ataaatttgc tggccat 1827 40 608 PRT Pseudomonas sp. 40 Met Ala Ser Lys Phe Ile Ser Leu Ala Glu Leu Ser Lys Thr Ala Pro 1 5 10 15 Lys Thr Leu Asn Arg Gly Ala Ile Glu Gln Leu Glu Pro Gly Ala Val 20 25 30 Pro Ser Leu His Asp Leu Thr Glu Ser Leu Leu Phe Ser Pro Gly Asp 35 40 45 Gly Arg Ile Trp Leu Asn Gly Ala Arg Met Leu Leu Leu His Ser Ser 50 55 60 Gly Leu Gly Ala Leu Arg Arg Glu Leu Ile Glu Ser Val Gly Phe Ala 65 70 75 80 Arg Ala Arg Gly Ile Met Leu Arg Thr Gly Tyr His Cys Gly Ala Lys 85 90 95 Asp Ala Ala Leu Leu Lys Glu Arg Phe Ser Asn Ala Asp Pro Leu Ser 100 105 110 Leu Phe Ala Ala Gly Pro Asn Leu His Ser Leu Glu Gly Ala Val Arg 115 120 125 Val Glu Pro Ile His Phe Asp Phe Asp Met Asp Leu Gly Thr Tyr Tyr 130 135 140 Gly Glu Phe Leu Trp His His Ser Ser Glu Asp Asp Glu His Ile Ala 145 150 155 160 His Tyr Gly Ile Gly Thr Glu Pro Ala Cys Trp Thr Gln Thr Gly Tyr 165 170 175 Ala Ile Gly Tyr Thr Ser Ala Leu Val Gly Arg Leu Ile Leu Tyr Arg 180 185 190 Glu Val Glu Cys Arg Ser Thr Gly Ser Gln Ile Cys Arg Met Ile Gly 195 200 205 Lys Pro Ala Glu Glu Trp Glu Asp Ala Glu Glu Asp Leu Ala Ala Leu 210 215 220 Ser Ala Thr Ile Cys Arg Gln Pro Ser Thr Pro Gln Arg Arg Arg Thr 225 230 235 240 Gln Gln Gly Gln Arg Asn Thr Pro His His Ser Ala Ala Asp Ser Ser 245 250 255 Thr Ala Ser Glu His Asp Met Val Gly Ile Ser Ser Ala Phe Asn Ala 260 265 270 Ala Cys His Met Leu Lys Arg Val Ala Pro Thr Glu Ala Thr Val Leu 275 280 285 Phe Thr Gly Glu Ser Gly Val Gly Lys Glu Met Phe Ala Arg Met Leu 290 295 300 His Arg Ile Ser Pro Arg His Asp Gly Pro Phe Val Ala Val Asn Cys 305 310 315 320 Ala Arg Ile Pro Glu Thr Leu Met Glu Ser Glu Leu Phe Gly Val Glu 325 330 335 Arg Gly Ala Phe Thr Gly Ala Thr Gln Ser Arg Ala Gly Arg Phe Glu 340 345 350 Arg Ala Ser Gly Gly Thr Leu Phe Leu Asp Glu Ile Gly Thr Leu Ser 355 360 365 Leu Val Ala Gln Gly Lys Leu Leu Arg Ala Leu Gln Glu Ser Glu Val 370 375 380 Glu Arg Val Gly Gly Ser Arg Thr Leu Lys Val Asp Val Arg Val Val 385 390 395 400 Ala Ala Thr Asn Val Asp Leu Arg Ala Gly Val Gln Arg Gly Glu Phe 405 410 415 Arg Glu Asp Leu Phe Phe Arg Leu Asn Val Tyr Pro Ile His Leu Pro 420 425 430 Pro Leu Arg Glu Arg Lys Glu Asp Ile Pro Leu Leu Met Thr Tyr Phe 435 440 445 Leu His Arg Phe Asn Gln Arg His Ser Arg Val Val Ser Gly Phe Thr 450 455 460 Pro Arg Ala Ala Asn Ala Leu Leu Gly Tyr Asp Phe Pro Gly Asn Ile 465 470 475 480 Arg Glu Leu Gln Asn Leu Val Glu Arg Gly Val Ile Ser Ala Pro Glu 485 490 495 Asp Gly Ala Ile Asp Val Ser His Leu Phe Thr Ser Gly Glu Arg Leu 500 505 510 Thr Gln Pro Met Phe Ser Ile Gly Ala Ser Gly Ile Leu Ala Thr Ala 515 520 525 Gln Asp Asn Gln Pro Gln Glu Thr Gly Ser Pro Ser Thr Ser Phe Ser 530 535 540 Lys Pro Thr Ser Thr Ile Asp Ser Pro Leu Asp Ser Lys Asp Leu Ser 545 550 555 560 Ala Met Thr Leu Gln Glu Val Glu Asp Ala Met Ile Ser His Ala Leu 565 570 575 Gly Gln Val Lys Gly Asn Val Ser Glu Ala Ala Arg Arg Leu Gly Leu 580 585 590 Thr Arg Ala Gln Leu Ser Tyr Arg Ile Ser Arg Arg Pro Leu Asp Lys 595 600 605 41 768 DNA Pseudomonas sp. CDS (1)..(765) product = ”Coniferylalkohol-Dehydrogenase“ / gene = ”cadh“ 41 atg caa ctg acc aac aag aaa atc gtc gtc acc gga gtg tcc tcc ggt 48 Met Gln Leu Thr Asn Lys Lys Ile Val Val Thr Gly Val Ser Ser Gly 1 5 10 15 atc ggt gcc gaa act gcc cgc gtt ctg cgc tct cac ggc gcc aca gtg 96 Ile Gly Ala Glu Thr Ala Arg Val Leu Arg Ser His Gly Ala Thr Val 20 25 30 att ggc gta gat cgc aac atg ccg agc ctg act ctg gat gct ttc gtt 144 Ile Gly Val Asp Arg Asn Met Pro Ser Leu Thr Leu Asp Ala Phe Val 35 40 45 cag gct gac ctg agc cat cct gaa ggc atc gat aag gcc atc tct cag 192 Gln Ala Asp Leu Ser His Pro Glu Gly Ile Asp Lys Ala Ile Ser Gln 50 55 60 ctg ccg gag aaa att gac gga ctc tgc aat atc gcc ggg gtg ccc ggc 240 Leu Pro Glu Lys Ile Asp Gly Leu Cys Asn Ile Ala Gly Val Pro Gly 65 70 75 80 act gcc gat cct cag ctc gtc gca aac gtg aac tac ctg ggt cta aag 288 Thr Ala Asp Pro Gln Leu Val Ala Asn Val Asn Tyr Leu Gly Leu Lys 85 90 95 tat ctg acc gag gca gtc ctg tcg cgc att caa ccc ggt ggt tcg att 336 Tyr Leu Thr Glu Ala Val Leu Ser Arg Ile Gln Pro Gly Gly Ser Ile 100 105 110 gtc aac gtg tcc tct gtg ctt ggc gcc gag tgg ccg gcc cgc ctt cag 384 Val Asn Val Ser Ser Val Leu Gly Ala Glu Trp Pro Ala Arg Leu Gln 115 120 125 ttg cat aag gag ctg ggg agt gtt gtt gga ttc tcc gaa ggc cag gca 432 Leu His Lys Glu Leu Gly Ser Val Val Gly Phe Ser Glu Gly Gln Ala 130 135 140 tgg ctt aag cag aat cca gtg gcc ccc gaa ttc tgc tac cag tat ttc 480 Trp Leu Lys Gln Asn Pro Val Ala Pro Glu Phe Cys Tyr Gln Tyr Phe 145 150 155 160 aaa gaa gca ctg atc gtt tgg tct caa gtt cag gcg cag gaa tgg ttc 528 Lys Glu Ala Leu Ile Val Trp Ser Gln Val Gln Ala Gln Glu Trp Phe 165 170 175 atg agg acg tct gta cgc atg aac tgc atc gcc ccc ggc cct gta ttc 576 Met Arg Thr Ser Val Arg Met Asn Cys Ile Ala Pro Gly Pro Val Phe 180 185 190 act ccc att ctc aat gag ttc gtc acc atg ctg ggt caa gag cgg act 624 Thr Pro Ile Leu Asn Glu Phe Val Thr Met Leu Gly Gln Glu Arg Thr 195 200 205 cag gcg gac gct cat cgt att aag cgc cca gca tat gcc gat gaa gtg 672 Gln Ala Asp Ala His Arg Ile Lys Arg Pro Ala Tyr Ala Asp Glu Val 210 215 220 gcc gcg gtg att gca ttc atg tgt gct gag gag tca cgt tgg atc aac 720 Ala Ala Val Ile Ala Phe Met Cys Ala Glu Glu Ser Arg Trp Ile Asn 225 230 235 240 ggc ata aat att cca gtg gac gga ggt ttg gca tcg acc tac gtg taa 768 Gly Ile Asn Ile Pro Val Asp Gly Gly Leu Ala Ser Thr Tyr Val 245 250 255 42 255 PRT Pseudomonas sp. 42 Met Gln Leu Thr Asn Lys Lys Ile Val Val Thr Gly Val Ser Ser Gly 1 5 10 15 Ile Gly Ala Glu Thr Ala Arg Val Leu Arg Ser His Gly Ala Thr Val 20 25 30 Ile Gly Val Asp Arg Asn Met Pro Ser Leu Thr Leu Asp Ala Phe Val 35 40 45 Gln Ala Asp Leu Ser His Pro Glu Gly Ile Asp Lys Ala Ile Ser Gln 50 55 60 Leu Pro Glu Lys Ile Asp Gly Leu Cys Asn Ile Ala Gly Val Pro Gly 65 70 75 80 Thr Ala Asp Pro Gln Leu Val Ala Asn Val Asn Tyr Leu Gly Leu Lys 85 90 95 Tyr Leu Thr Glu Ala Val Leu Ser Arg Ile Gln Pro Gly Gly Ser Ile 100 105 110 Val Asn Val Ser Ser Val Leu Gly Ala Glu Trp Pro Ala Arg Leu Gln 115 120 125 Leu His Lys Glu Leu Gly Ser Val Val Gly Phe Ser Glu Gly Gln Ala 130 135 140 Trp Leu Lys Gln Asn Pro Val Ala Pro Glu Phe Cys Tyr Gln Tyr Phe 145 150 155 160 Lys Glu Ala Leu Ile Val Trp Ser Gln Val Gln Ala Gln Glu Trp Phe 165 170 175 Met Arg Thr Ser Val Arg Met Asn Cys Ile Ala Pro Gly Pro Val Phe 180 185 190 Thr Pro Ile Leu Asn Glu Phe Val Thr Met Leu Gly Gln Glu Arg Thr 195 200 205 Gln Ala Asp Ala His Arg Ile Lys Arg Pro Ala Tyr Ala Asp Glu Val 210 215 220 Ala Ala Val Ile Ala Phe Met Cys Ala Glu Glu Ser Arg Trp Ile Asn 225 230 235 240 Gly Ile Asn Ile Pro Val Asp Gly Gly Leu Ala Ser Thr Tyr Val 245 250 255 43 26 DNA Pseudomonas sp. 43 atgcarctba cbaayaaraa ratygt 26 44 20 PRT Pseudomonas sp. UNSURE (12) UNSURE (13)..(19) 44 Met Gln Leu Thr Asn Lys Lys Ile Val Val Val Xaa Val Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Xaa 20 45 20 PRT Pseudomonas sp. UNSURE (20) 45 Ser Ile Leu Gly Leu Asn Gly Ala Pro Val Gly Ala Glu Gln Leu Gly 1 5 10 15 Ser Ala Leu Xaa 20 

1. Synthetic enzymes for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid from eugenol.
 2. Synthetic enzymes according to claim 1 selected from the group comprising: a) eugenol hydroxylase, b) coniferyl alcohol dehydrogenase, c) coniferylaldehyde dehydrogenase, d) ferulic acid deacylase and e) vanillin dehydrogenase.
 3. DNA coding for the enzymes according to claims 1 and 2 as well as partial sequences and functional equivalents thereof.
 4. Cosmid clones containing the DNA according to claim
 3. 5. Vectors containing the DNA according to claim
 3. 6. Microorganisms transformed with the DNA according to claim
 3. 7. The use of the DNA according to claim 3 for the transformation of microorganisms.
 8. The use of microorganisms according to claim 6 for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid.
 9. A process for the production of coniferyl alcohol from eugenol, characterised in that the reaction is carried out in the presence of eugenol hydroxylase.
 10. A process for the production of coniferylaldehyde from coniferyl alcohol, characterised in that the reaction is carried out in the presence of coniferyl alcohol dehydrogenase.
 11. A process for the production of ferulic acid from coniferylaldehyde, characterised in that the reaction is carried out in the presence of coniferylaldehyde dehydrogenase.
 12. A process for the production of vanillin from ferulic acid, characterised in that the reaction is carried out in the presence of ferulic acid deacylase.
 13. A process for the production of vanillic acid from vanillin, characterised in that the reaction is carried out in the presence of vanillin dehydrogenase. 